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Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

Cytofluorimetric evaluation of lipid hydroperoxides. The A549 cells incubated for 18 h with FFBJ or FFOJ were oxidatively stressed with H2O2 200 μM for 2 h and then loaded by DPPP probe. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. Data represent the mean ± SEM of at least three separate experiments. ∗∗∗P < 0.001 versus control cells and ##P < 0.01 versus H2O2-treated cells.
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fig5: Cytofluorimetric evaluation of lipid hydroperoxides. The A549 cells incubated for 18 h with FFBJ or FFOJ were oxidatively stressed with H2O2 200 μM for 2 h and then loaded by DPPP probe. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. Data represent the mean ± SEM of at least three separate experiments. ∗∗∗P < 0.001 versus control cells and ##P < 0.01 versus H2O2-treated cells.

Mentions: The high lipid content of cell membranes makes them particularly susceptible to oxidative damage. We therefore measured the lipid hydroperoxides to investigate the consequences of the oxidative damage caused by H2O2 on the membrane lipids and also to examine the effect of our extracts. The level of lipid peroxidation in A549 cells increased up to 6-fold after 2 h incubation with 200 μM of H2O2, an effect counteracted by preincubation with either extract (Figure 5). Interestingly, the DPPP emission value detected in H2O2-stressed cells were lowered by up to 40 and 55% by pretreatment with FFBJ at concentrations of 25 and 50 μg/mL, respectively (P < 0.01). Similar results were obtained using FFOJ (45 and 60% emission DPPP reduction by 25 and 50 μg/mL concentrations, resp.; P < 0.01).


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Cytofluorimetric evaluation of lipid hydroperoxides. The A549 cells incubated for 18 h with FFBJ or FFOJ were oxidatively stressed with H2O2 200 μM for 2 h and then loaded by DPPP probe. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. Data represent the mean ± SEM of at least three separate experiments. ∗∗∗P < 0.001 versus control cells and ##P < 0.01 versus H2O2-treated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499611&req=5

fig5: Cytofluorimetric evaluation of lipid hydroperoxides. The A549 cells incubated for 18 h with FFBJ or FFOJ were oxidatively stressed with H2O2 200 μM for 2 h and then loaded by DPPP probe. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. Data represent the mean ± SEM of at least three separate experiments. ∗∗∗P < 0.001 versus control cells and ##P < 0.01 versus H2O2-treated cells.
Mentions: The high lipid content of cell membranes makes them particularly susceptible to oxidative damage. We therefore measured the lipid hydroperoxides to investigate the consequences of the oxidative damage caused by H2O2 on the membrane lipids and also to examine the effect of our extracts. The level of lipid peroxidation in A549 cells increased up to 6-fold after 2 h incubation with 200 μM of H2O2, an effect counteracted by preincubation with either extract (Figure 5). Interestingly, the DPPP emission value detected in H2O2-stressed cells were lowered by up to 40 and 55% by pretreatment with FFBJ at concentrations of 25 and 50 μg/mL, respectively (P < 0.01). Similar results were obtained using FFOJ (45 and 60% emission DPPP reduction by 25 and 50 μg/mL concentrations, resp.; P < 0.01).

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus