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Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

Confocal laser scanning microscope images of DCF-DA-stained cells. A549 cells grown on cell slides were preincubated with FFBJ or FFOJ and after 18 h exposed to H2O2 200 μM. Green fluorescence represented the amounts of ROS. Images shown are representative of three independent experiments. 400x magnification.
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fig4: Confocal laser scanning microscope images of DCF-DA-stained cells. A549 cells grown on cell slides were preincubated with FFBJ or FFOJ and after 18 h exposed to H2O2 200 μM. Green fluorescence represented the amounts of ROS. Images shown are representative of three independent experiments. 400x magnification.

Mentions: The data from FACS analyses were confirmed by CLSM observations. Figure 4 shows that, in comparison to oxidatively stressed A549 cells, the presence of Citrus extracts dampened the fluorescence emission proportionally to the concentration employed.


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Confocal laser scanning microscope images of DCF-DA-stained cells. A549 cells grown on cell slides were preincubated with FFBJ or FFOJ and after 18 h exposed to H2O2 200 μM. Green fluorescence represented the amounts of ROS. Images shown are representative of three independent experiments. 400x magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499611&req=5

fig4: Confocal laser scanning microscope images of DCF-DA-stained cells. A549 cells grown on cell slides were preincubated with FFBJ or FFOJ and after 18 h exposed to H2O2 200 μM. Green fluorescence represented the amounts of ROS. Images shown are representative of three independent experiments. 400x magnification.
Mentions: The data from FACS analyses were confirmed by CLSM observations. Figure 4 shows that, in comparison to oxidatively stressed A549 cells, the presence of Citrus extracts dampened the fluorescence emission proportionally to the concentration employed.

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus