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Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

Effect of FFBJ or FFOJ on cell death induced by H2O2. The cells were treated for 18 h with FFBJ or FFOJ followed by incubation with 200 μM H2O2 for additional 2 h. PI-positive cells were determined by flow cytometry collecting the emission signal in the FL-3 channel. Data represent means ± SEM of three separate experiments. ∗∗∗P < 0.001 versus control cells and #P < 0.05 versus H2O2-treated cells.
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fig3: Effect of FFBJ or FFOJ on cell death induced by H2O2. The cells were treated for 18 h with FFBJ or FFOJ followed by incubation with 200 μM H2O2 for additional 2 h. PI-positive cells were determined by flow cytometry collecting the emission signal in the FL-3 channel. Data represent means ± SEM of three separate experiments. ∗∗∗P < 0.001 versus control cells and #P < 0.05 versus H2O2-treated cells.

Mentions: To study the potential protective effects of both FFBJ and FFOJ in different compartments of oxidatively injured A549 lung epithelial cells, we first measured the intracellular content of ROS and checked cell viability in H2O2-stressed or unstressed cells, which had either been pretreated or not with Citrus extracts. As expected, DCF emission values in cells treated with FFBJ and FFOJ for 18 h did not differ significantly from the background values recorded in untreated cells (Figure 2). Similarly, no differences between FFBJ- or FFOJ-treated and untreated cells were recorded in PI emission values (Figure 3). These results indicate that the extracts at both 25 and 50 μg/mL concentrations did not trigger ROS generation or affect cell viability (always >90%). Instead, DCF emission values in H2O2-stressed cells were up to 6.7-fold higher than those detected in untreated cultures, suggesting that there had been an increase in ROS generation (Figure 2). Consequently, H2O2 caused 25% cell death, as detected by FACS analysis (Figure 3). Interestingly, as shown in Figures 2(a) and 2(b), the presence of Citrus extracts reduced H2O2-induced oxidative stress, thus preventing the increase in ROS. Indeed, the fluorescence emission curve for untreated culture shows a consistent peak (proportional to the number of cells with low emission values) on the left of the graph. In contrast, in A549 cells incubated with 200 μM H2O2 for 2 h a larger number of cells with high emission values were detected (the gray peak on the right), indicating an increased ROS production.


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Effect of FFBJ or FFOJ on cell death induced by H2O2. The cells were treated for 18 h with FFBJ or FFOJ followed by incubation with 200 μM H2O2 for additional 2 h. PI-positive cells were determined by flow cytometry collecting the emission signal in the FL-3 channel. Data represent means ± SEM of three separate experiments. ∗∗∗P < 0.001 versus control cells and #P < 0.05 versus H2O2-treated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Effect of FFBJ or FFOJ on cell death induced by H2O2. The cells were treated for 18 h with FFBJ or FFOJ followed by incubation with 200 μM H2O2 for additional 2 h. PI-positive cells were determined by flow cytometry collecting the emission signal in the FL-3 channel. Data represent means ± SEM of three separate experiments. ∗∗∗P < 0.001 versus control cells and #P < 0.05 versus H2O2-treated cells.
Mentions: To study the potential protective effects of both FFBJ and FFOJ in different compartments of oxidatively injured A549 lung epithelial cells, we first measured the intracellular content of ROS and checked cell viability in H2O2-stressed or unstressed cells, which had either been pretreated or not with Citrus extracts. As expected, DCF emission values in cells treated with FFBJ and FFOJ for 18 h did not differ significantly from the background values recorded in untreated cells (Figure 2). Similarly, no differences between FFBJ- or FFOJ-treated and untreated cells were recorded in PI emission values (Figure 3). These results indicate that the extracts at both 25 and 50 μg/mL concentrations did not trigger ROS generation or affect cell viability (always >90%). Instead, DCF emission values in H2O2-stressed cells were up to 6.7-fold higher than those detected in untreated cultures, suggesting that there had been an increase in ROS generation (Figure 2). Consequently, H2O2 caused 25% cell death, as detected by FACS analysis (Figure 3). Interestingly, as shown in Figures 2(a) and 2(b), the presence of Citrus extracts reduced H2O2-induced oxidative stress, thus preventing the increase in ROS. Indeed, the fluorescence emission curve for untreated culture shows a consistent peak (proportional to the number of cells with low emission values) on the left of the graph. In contrast, in A549 cells incubated with 200 μM H2O2 for 2 h a larger number of cells with high emission values were detected (the gray peak on the right), indicating an increased ROS production.

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus