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Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of flavonoids found in FFBJ and FFOJ. aRutinose (Ru). bNeohesperidose (Nh). Methyl group (Me). Glucoside (Glu).
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fig1: Chemical structures of flavonoids found in FFBJ and FFOJ. aRutinose (Ru). bNeohesperidose (Nh). Methyl group (Me). Glucoside (Glu).

Mentions: The chemical composition of the FFBJ and FFOJ extracts used in this study are shown in Table 1 and expressed in mg/g of dried extract. Figure 1 shows the chemical structures of the flavonoids detected. As expected, neohesperidin, naringin, hesperetin, and melitidin were the main compounds present in FFBJ, although neoeriocitrin and naringenin were also present in quite large quantities. In FFOJ the highest amounts of flavonoids present were hesperidin, narirutin, and vicenin-2. Although both extracts belong to the Citrus family, FFBJ and FFOJ differed regarding their abundance in flavonoids and the relative percentages (Table 1). For instance, some compounds present in FFBJ were not present or undetectable in FFOJ, while hesperidin and narirutin were abundant in FFOJ and scarce in FFBJ. A slight difference in chemical composition may reflect significant differences in both physicochemical properties and biological activities.


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Chemical structures of flavonoids found in FFBJ and FFOJ. aRutinose (Ru). bNeohesperidose (Nh). Methyl group (Me). Glucoside (Glu).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499611&req=5

fig1: Chemical structures of flavonoids found in FFBJ and FFOJ. aRutinose (Ru). bNeohesperidose (Nh). Methyl group (Me). Glucoside (Glu).
Mentions: The chemical composition of the FFBJ and FFOJ extracts used in this study are shown in Table 1 and expressed in mg/g of dried extract. Figure 1 shows the chemical structures of the flavonoids detected. As expected, neohesperidin, naringin, hesperetin, and melitidin were the main compounds present in FFBJ, although neoeriocitrin and naringenin were also present in quite large quantities. In FFOJ the highest amounts of flavonoids present were hesperidin, narirutin, and vicenin-2. Although both extracts belong to the Citrus family, FFBJ and FFOJ differed regarding their abundance in flavonoids and the relative percentages (Table 1). For instance, some compounds present in FFBJ were not present or undetectable in FFOJ, while hesperidin and narirutin were abundant in FFOJ and scarce in FFBJ. A slight difference in chemical composition may reflect significant differences in both physicochemical properties and biological activities.

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus