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Effects of Lupenone, Lupeol, and Taraxerol Derived from Adenophora triphylla on the Gene Expression and Production of Airway MUC5AC Mucin.

Yoon YP, Lee HJ, Lee DU, Lee SK, Hong JH, Lee CJ - Tuberc Respir Dis (Seoul) (2015)

Bottom Line: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively.The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Background: Adenophora triphylla var. japonica is empirically used for controlling airway inflammatory diseases in folk medicine. We evaluated the gene expression and production of mucin from airway epithelial cells in response to lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica.

Methods: Confluent NCI-H292 cells were pretreated with lupenone, lupeol or taraxerol for 30 minutes and then stimulated with tumor necrosis factor α (TNF-α) for 24 hours. The MUC5AC mucin gene expression and production were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Additionally, we examined whether lupenone, lupeol or taraxerol affects MUC5AC mucin production induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA), the other 2 stimulators of airway mucin production.

Results: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively. The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.

Conclusion: These results indicated that lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica regulates the production and gene expression of mucin, by directly acting on airway epithelial cells. In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of lupenone (A), lupeol (B), or taraxerol (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC mucin production in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by enzyme-linked immunosorbent assay. Each bar represents a mean±SEM of three culture wells in comparison with that of control set at 100% (A-C). Three independent experiments were performed and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05).
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Figure 5: Effect of lupenone (A), lupeol (B), or taraxerol (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC mucin production in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by enzyme-linked immunosorbent assay. Each bar represents a mean±SEM of three culture wells in comparison with that of control set at 100% (A-C). Three independent experiments were performed and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05).

Mentions: Lupenone significantly inhibited PMA-induced MUC5AC production from NCI-H292 cells. The amounts of mucin in the cells of lupenone-treated cultures were 100±14%, 247±2%, 261±1%, 147±8%, and 55±3% for control, 10 ng/mL of PMA alone, PMA plus lupenone 10-6 M, PMA plus lupenone 10-5 M, and PMA plus lupenone 10-4 M, respectively (Figure 5A).


Effects of Lupenone, Lupeol, and Taraxerol Derived from Adenophora triphylla on the Gene Expression and Production of Airway MUC5AC Mucin.

Yoon YP, Lee HJ, Lee DU, Lee SK, Hong JH, Lee CJ - Tuberc Respir Dis (Seoul) (2015)

Effect of lupenone (A), lupeol (B), or taraxerol (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC mucin production in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by enzyme-linked immunosorbent assay. Each bar represents a mean±SEM of three culture wells in comparison with that of control set at 100% (A-C). Three independent experiments were performed and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499588&req=5

Figure 5: Effect of lupenone (A), lupeol (B), or taraxerol (C) on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC mucin production in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by enzyme-linked immunosorbent assay. Each bar represents a mean±SEM of three culture wells in comparison with that of control set at 100% (A-C). Three independent experiments were performed and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05).
Mentions: Lupenone significantly inhibited PMA-induced MUC5AC production from NCI-H292 cells. The amounts of mucin in the cells of lupenone-treated cultures were 100±14%, 247±2%, 261±1%, 147±8%, and 55±3% for control, 10 ng/mL of PMA alone, PMA plus lupenone 10-6 M, PMA plus lupenone 10-5 M, and PMA plus lupenone 10-4 M, respectively (Figure 5A).

Bottom Line: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively.The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Background: Adenophora triphylla var. japonica is empirically used for controlling airway inflammatory diseases in folk medicine. We evaluated the gene expression and production of mucin from airway epithelial cells in response to lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica.

Methods: Confluent NCI-H292 cells were pretreated with lupenone, lupeol or taraxerol for 30 minutes and then stimulated with tumor necrosis factor α (TNF-α) for 24 hours. The MUC5AC mucin gene expression and production were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Additionally, we examined whether lupenone, lupeol or taraxerol affects MUC5AC mucin production induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA), the other 2 stimulators of airway mucin production.

Results: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively. The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.

Conclusion: These results indicated that lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica regulates the production and gene expression of mucin, by directly acting on airway epithelial cells. In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

No MeSH data available.


Related in: MedlinePlus