Limits...
Effects of Lupenone, Lupeol, and Taraxerol Derived from Adenophora triphylla on the Gene Expression and Production of Airway MUC5AC Mucin.

Yoon YP, Lee HJ, Lee DU, Lee SK, Hong JH, Lee CJ - Tuberc Respir Dis (Seoul) (2015)

Bottom Line: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively.The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Background: Adenophora triphylla var. japonica is empirically used for controlling airway inflammatory diseases in folk medicine. We evaluated the gene expression and production of mucin from airway epithelial cells in response to lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica.

Methods: Confluent NCI-H292 cells were pretreated with lupenone, lupeol or taraxerol for 30 minutes and then stimulated with tumor necrosis factor α (TNF-α) for 24 hours. The MUC5AC mucin gene expression and production were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Additionally, we examined whether lupenone, lupeol or taraxerol affects MUC5AC mucin production induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA), the other 2 stimulators of airway mucin production.

Results: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively. The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.

Conclusion: These results indicated that lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica regulates the production and gene expression of mucin, by directly acting on airway epithelial cells. In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of lupenone (A), lupeol (B), or taraxerol (C) on tumor necrosis factor α (TNF-α)-induced MUC5AC gene expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with TNF-α (0.2 nM) for 24 hours. MUC5AC gene expression was measured by reverse transcription-polymerase chain reaction. Three independent experiments were performed and the representative images were shown. Polymerase chain reaction images were quantified using densitometry and the graphic data were also shown. Each bar represents a mean±SEM of 3 densitograms in comparison with that of control set at 100% (A-C). *Significantly different from control (p<0.05). †Significantly different from TNF-α alone (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4499588&req=5

Figure 2: Effect of lupenone (A), lupeol (B), or taraxerol (C) on tumor necrosis factor α (TNF-α)-induced MUC5AC gene expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with TNF-α (0.2 nM) for 24 hours. MUC5AC gene expression was measured by reverse transcription-polymerase chain reaction. Three independent experiments were performed and the representative images were shown. Polymerase chain reaction images were quantified using densitometry and the graphic data were also shown. Each bar represents a mean±SEM of 3 densitograms in comparison with that of control set at 100% (A-C). *Significantly different from control (p<0.05). †Significantly different from TNF-α alone (p<0.05).

Mentions: As can be seen in Figure 2, MUC5AC gene expression induced by TNF-α from NCI-H292 cells was inhibited by pretreatment with lupenone, lupeol, and taraxerol, respectively (Figure 2). Cytotoxicity was checked by lactate dehydrogenase assay and there was no remarkable cytotoxic effect of lupenone, lupeol, or taraxerol, at the treatment concentrations (data not shown).


Effects of Lupenone, Lupeol, and Taraxerol Derived from Adenophora triphylla on the Gene Expression and Production of Airway MUC5AC Mucin.

Yoon YP, Lee HJ, Lee DU, Lee SK, Hong JH, Lee CJ - Tuberc Respir Dis (Seoul) (2015)

Effect of lupenone (A), lupeol (B), or taraxerol (C) on tumor necrosis factor α (TNF-α)-induced MUC5AC gene expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with TNF-α (0.2 nM) for 24 hours. MUC5AC gene expression was measured by reverse transcription-polymerase chain reaction. Three independent experiments were performed and the representative images were shown. Polymerase chain reaction images were quantified using densitometry and the graphic data were also shown. Each bar represents a mean±SEM of 3 densitograms in comparison with that of control set at 100% (A-C). *Significantly different from control (p<0.05). †Significantly different from TNF-α alone (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499588&req=5

Figure 2: Effect of lupenone (A), lupeol (B), or taraxerol (C) on tumor necrosis factor α (TNF-α)-induced MUC5AC gene expression in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of lupenone, lupeol, or taraxerol for 30 minutes and then stimulated with TNF-α (0.2 nM) for 24 hours. MUC5AC gene expression was measured by reverse transcription-polymerase chain reaction. Three independent experiments were performed and the representative images were shown. Polymerase chain reaction images were quantified using densitometry and the graphic data were also shown. Each bar represents a mean±SEM of 3 densitograms in comparison with that of control set at 100% (A-C). *Significantly different from control (p<0.05). †Significantly different from TNF-α alone (p<0.05).
Mentions: As can be seen in Figure 2, MUC5AC gene expression induced by TNF-α from NCI-H292 cells was inhibited by pretreatment with lupenone, lupeol, and taraxerol, respectively (Figure 2). Cytotoxicity was checked by lactate dehydrogenase assay and there was no remarkable cytotoxic effect of lupenone, lupeol, or taraxerol, at the treatment concentrations (data not shown).

Bottom Line: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively.The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Background: Adenophora triphylla var. japonica is empirically used for controlling airway inflammatory diseases in folk medicine. We evaluated the gene expression and production of mucin from airway epithelial cells in response to lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica.

Methods: Confluent NCI-H292 cells were pretreated with lupenone, lupeol or taraxerol for 30 minutes and then stimulated with tumor necrosis factor α (TNF-α) for 24 hours. The MUC5AC mucin gene expression and production were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Additionally, we examined whether lupenone, lupeol or taraxerol affects MUC5AC mucin production induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA), the other 2 stimulators of airway mucin production.

Results: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-α from NCI-H292 cells, respectively. The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells.

Conclusion: These results indicated that lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica regulates the production and gene expression of mucin, by directly acting on airway epithelial cells. In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

No MeSH data available.


Related in: MedlinePlus