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Expression Pattern of Alternative Splicing Variants of Human Telomerase Reverse Transcriptase (hTERT) in Cancer Cell Lines Was not Associated with the Origin of the Cells.

Khosravi-Maharlooei M, Jaberipour M, Hosseini Tashnizi A, Attar A, Amirmoezi F, Habibagahi M - Int J Mol Cell Med (2015)

Bottom Line: Relative assessment of hTERT expression showed greater expression of the α-deleted variant in the telomerase negative cells (P= 0.04).Those cells possessed the α/β-deleted variant to a smaller extent when compared to the cells with telomerase activity.In spite that, the regulatory roles of hTERT on telomerase activity is still a potential to be utilized as targets for cancer therapies.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Cell and Molecular Medicine Research Group, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
Telomerase and systems controlling their activity have been of great attention. There are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. Therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of hTERT and doubling time of a series of cancer cell lines originated from hematopoietic, breast, colorectal, neural, ovarian, lung, kidney, bladder, prostate and head and neck cancers were investigated. Expression levels of four different variants of hTERT (the full length, α-deletion, β-deletion and α/β-deletion) were quantitatively measured by real time PCR. Telomerase activity was determined by the telomerase repeat amplification protocol (TRAP) while doubling time of the cells measured by plotting growth curves. Results showed high diversity in the relative proportions of hTERT transcripts while the majority of the cells expressed the full length variant as the main transcript. Telomerase activity could not be detected in all cells. Relative assessment of hTERT expression showed greater expression of the α-deleted variant in the telomerase negative cells (P= 0.04). Those cells possessed the α/β-deleted variant to a smaller extent when compared to the cells with telomerase activity. Greater association between full length spliced variant and β-variant expression was observed in cells presenting telomerase activity (P= 0.0007, r= 0.74). High degrees of variation among the studied cells regarding the pattern of hTERT expression were present. In spite that, the regulatory roles of hTERT on telomerase activity is still a potential to be utilized as targets for cancer therapies.

No MeSH data available.


Related in: MedlinePlus

Doubling time of cancer cell lines Doubling time of different cancer cell lines were estimated by plotting the corresponding growth curves. The calculated time was used as index of proliferative capacity of the cell lines
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Figure 2: Doubling time of cancer cell lines Doubling time of different cancer cell lines were estimated by plotting the corresponding growth curves. The calculated time was used as index of proliferative capacity of the cell lines

Mentions: Doubling times of the studied cell lines were measured by counting cells in the cultures and plotting growth curves. Figure 2 summarizes the doubling times of the 20 studied cell lines. As it is shown, KG-1 cells with myeloid origin had the longest doubling time (70 hours) which corresponds to slow proliferation kinetics of this cell line. On the other hand, LNCaP the prostate carcinoma cell showed the most rapid proliferation with doubling time of 28 hours followed by CACO2 and LS180 with doubling time of 30 h.


Expression Pattern of Alternative Splicing Variants of Human Telomerase Reverse Transcriptase (hTERT) in Cancer Cell Lines Was not Associated with the Origin of the Cells.

Khosravi-Maharlooei M, Jaberipour M, Hosseini Tashnizi A, Attar A, Amirmoezi F, Habibagahi M - Int J Mol Cell Med (2015)

Doubling time of cancer cell lines Doubling time of different cancer cell lines were estimated by plotting the corresponding growth curves. The calculated time was used as index of proliferative capacity of the cell lines
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499573&req=5

Figure 2: Doubling time of cancer cell lines Doubling time of different cancer cell lines were estimated by plotting the corresponding growth curves. The calculated time was used as index of proliferative capacity of the cell lines
Mentions: Doubling times of the studied cell lines were measured by counting cells in the cultures and plotting growth curves. Figure 2 summarizes the doubling times of the 20 studied cell lines. As it is shown, KG-1 cells with myeloid origin had the longest doubling time (70 hours) which corresponds to slow proliferation kinetics of this cell line. On the other hand, LNCaP the prostate carcinoma cell showed the most rapid proliferation with doubling time of 28 hours followed by CACO2 and LS180 with doubling time of 30 h.

Bottom Line: Relative assessment of hTERT expression showed greater expression of the α-deleted variant in the telomerase negative cells (P= 0.04).Those cells possessed the α/β-deleted variant to a smaller extent when compared to the cells with telomerase activity.In spite that, the regulatory roles of hTERT on telomerase activity is still a potential to be utilized as targets for cancer therapies.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Cell and Molecular Medicine Research Group, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
Telomerase and systems controlling their activity have been of great attention. There are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. Therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of hTERT and doubling time of a series of cancer cell lines originated from hematopoietic, breast, colorectal, neural, ovarian, lung, kidney, bladder, prostate and head and neck cancers were investigated. Expression levels of four different variants of hTERT (the full length, α-deletion, β-deletion and α/β-deletion) were quantitatively measured by real time PCR. Telomerase activity was determined by the telomerase repeat amplification protocol (TRAP) while doubling time of the cells measured by plotting growth curves. Results showed high diversity in the relative proportions of hTERT transcripts while the majority of the cells expressed the full length variant as the main transcript. Telomerase activity could not be detected in all cells. Relative assessment of hTERT expression showed greater expression of the α-deleted variant in the telomerase negative cells (P= 0.04). Those cells possessed the α/β-deleted variant to a smaller extent when compared to the cells with telomerase activity. Greater association between full length spliced variant and β-variant expression was observed in cells presenting telomerase activity (P= 0.0007, r= 0.74). High degrees of variation among the studied cells regarding the pattern of hTERT expression were present. In spite that, the regulatory roles of hTERT on telomerase activity is still a potential to be utilized as targets for cancer therapies.

No MeSH data available.


Related in: MedlinePlus