Limits...
APOBEC3 Deletion is Associated with Breast Cancer Risk in a Sample of Southeast Iranian Population.

Rezaei M, Hashemi M, Hashemi SM, Mashhadi MA, Taheri M - Int J Mol Cell Med (2015)

Bottom Line: Previous studies showed that apolipoprotein B mRNA- editing catalytic polypeptide-like 3 (APOBEC3) gene deletion significantly increased the risk of BC risk in Chinese and European women.The findings of this study showed that I/D as well as I/D+D/D genotype increased the risk of BC (OR= 1.57, 95% CI= 1.07- 2.31, p= 0.025 and OR= 1.50, 95% CI= 1.03- 2.19, p= 0.037, respectively) in comparison with I/I genotype.In conclusion, our findings suggest that APOBEC3 deletion polymorphism increased the risk of BC in an Iranian population in the southeast of Iran.

View Article: PubMed Central - PubMed

Affiliation: 1.Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. ; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

ABSTRACT
Breast Cancer (BC) is considered as one of the most important causes of death worldwide. Previous studies showed that apolipoprotein B mRNA- editing catalytic polypeptide-like 3 (APOBEC3) gene deletion significantly increased the risk of BC risk in Chinese and European women. The present study aimed to assess the possible impact of APOBEC3 deletion and the risk of BC in a sample of Iranian population. The APOBEC3 insertion/deletion (I/D) was analyzed in a case- control study including 262 BC patients and 217 healthy women. Polymerase chain reaction (PCR) was used to genotype the variant in APOBEC3 gene. The findings of this study showed that I/D as well as I/D+D/D genotype increased the risk of BC (OR= 1.57, 95% CI= 1.07- 2.31, p= 0.025 and OR= 1.50, 95% CI= 1.03- 2.19, p= 0.037, respectively) in comparison with I/I genotype. In conclusion, our findings suggest that APOBEC3 deletion polymorphism increased the risk of BC in an Iranian population in the southeast of Iran.

No MeSH data available.


Related in: MedlinePlus

Photograph of DNA electophoresis for detection of APOBEC3 I/D variant. M: DNA marker; lanes 1, and 3: I/D; lane 2, and  5: D/D; lanes 4 and 6, I/I genotypes
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4499572&req=5

Figure 1: Photograph of DNA electophoresis for detection of APOBEC3 I/D variant. M: DNA marker; lanes 1, and 3: I/D; lane 2, and  5: D/D; lanes 4 and 6, I/I genotypes

Mentions: PCR was performed using 2X Prime Taq Premix (Genet Bio, Korea). The amplification procedure consisted of an initial denaturing step for 5 min at 95 °C followed by 34 cycles: for 30 s at 95 °C, 24 s at 61 °C, and 17 s at 72 °C, as well as a final extension step for 10 min at 72 °C. The PCR products were visualized on a 3% agarose gel containing 0.5 µg/ ml of ethidium bromide (Figure 1).


APOBEC3 Deletion is Associated with Breast Cancer Risk in a Sample of Southeast Iranian Population.

Rezaei M, Hashemi M, Hashemi SM, Mashhadi MA, Taheri M - Int J Mol Cell Med (2015)

Photograph of DNA electophoresis for detection of APOBEC3 I/D variant. M: DNA marker; lanes 1, and 3: I/D; lane 2, and  5: D/D; lanes 4 and 6, I/I genotypes
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499572&req=5

Figure 1: Photograph of DNA electophoresis for detection of APOBEC3 I/D variant. M: DNA marker; lanes 1, and 3: I/D; lane 2, and  5: D/D; lanes 4 and 6, I/I genotypes
Mentions: PCR was performed using 2X Prime Taq Premix (Genet Bio, Korea). The amplification procedure consisted of an initial denaturing step for 5 min at 95 °C followed by 34 cycles: for 30 s at 95 °C, 24 s at 61 °C, and 17 s at 72 °C, as well as a final extension step for 10 min at 72 °C. The PCR products were visualized on a 3% agarose gel containing 0.5 µg/ ml of ethidium bromide (Figure 1).

Bottom Line: Previous studies showed that apolipoprotein B mRNA- editing catalytic polypeptide-like 3 (APOBEC3) gene deletion significantly increased the risk of BC risk in Chinese and European women.The findings of this study showed that I/D as well as I/D+D/D genotype increased the risk of BC (OR= 1.57, 95% CI= 1.07- 2.31, p= 0.025 and OR= 1.50, 95% CI= 1.03- 2.19, p= 0.037, respectively) in comparison with I/I genotype.In conclusion, our findings suggest that APOBEC3 deletion polymorphism increased the risk of BC in an Iranian population in the southeast of Iran.

View Article: PubMed Central - PubMed

Affiliation: 1.Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. ; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

ABSTRACT
Breast Cancer (BC) is considered as one of the most important causes of death worldwide. Previous studies showed that apolipoprotein B mRNA- editing catalytic polypeptide-like 3 (APOBEC3) gene deletion significantly increased the risk of BC risk in Chinese and European women. The present study aimed to assess the possible impact of APOBEC3 deletion and the risk of BC in a sample of Iranian population. The APOBEC3 insertion/deletion (I/D) was analyzed in a case- control study including 262 BC patients and 217 healthy women. Polymerase chain reaction (PCR) was used to genotype the variant in APOBEC3 gene. The findings of this study showed that I/D as well as I/D+D/D genotype increased the risk of BC (OR= 1.57, 95% CI= 1.07- 2.31, p= 0.025 and OR= 1.50, 95% CI= 1.03- 2.19, p= 0.037, respectively) in comparison with I/I genotype. In conclusion, our findings suggest that APOBEC3 deletion polymorphism increased the risk of BC in an Iranian population in the southeast of Iran.

No MeSH data available.


Related in: MedlinePlus