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Detection of Myocardial Metabolic Abnormalities by 18F-FDG PET/CT and Corresponding Pathological Changes in Beagles with Local Heart Irradiation.

Yan R, Song J, Wu Z, Guo M, Liu J, Li J, Hao X, Li S - Korean J Radiol (2015)

Bottom Line: High FDG uptake in irradiation field was detected in 33.3% (4/12) of 12H-F protocol and 83.3% (10/12) of F-HFD protocol in visual analysis, respectively (p = 0.031).The pathology of the irradiated myocardium showed obvious perivascular fibrosis and changes in mitochondrial vacuoles.An F-HFD preparation protocol used before obtaining PET/CT can improve the sensitivity of the detection of cardiotoxicity associated with radiotherapy.

View Article: PubMed Central - PubMed

Affiliation: Nursing College of Shanxi Medical University, Taiyuan 030001, China. ; Department of Nuclear Medicine, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT

Objective: To determine the efficacy of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) in the detection of radiation-induced myocardial damage in beagles by comparing two pre-scan preparation protocols as well as to determine the correlation between abnormal myocardial FDG uptake and pathological findings.

Materials and methods: The anterior myocardium of 12 beagles received radiotherapy locally with a single X-ray dose of 20 Gy. 18F-FDG cardiac PET/CT was performed at baseline and 3 months after radiation. Twelve beagles underwent two protocols before PET/CT: 12 hours of fasting (12H-F), 12H-F followed by a high-fat diet (F-HFD). Regions of interest were drawn on the irradiation and the non-irradiation fields to obtain their maximal standardized uptake values (SUVmax). Then the ratio of the SUV of the irradiation to the non-irradiation fields (INR) was computed. Histopathological changes were identified by light and electron microscopy.

Results: Using the 12H-F protocol, the average INRs were 1.18 ± 0.10 and 1.41 ± 0.18 before and after irradiation, respectively (p = 0.021). Using the F-HFD protocol, the average INRs were 0.99 ± 0.15 and 2.54 ± 0.43, respectively (p < 0.001). High FDG uptake in irradiation field was detected in 33.3% (4/12) of 12H-F protocol and 83.3% (10/12) of F-HFD protocol in visual analysis, respectively (p = 0.031). The pathology of the irradiated myocardium showed obvious perivascular fibrosis and changes in mitochondrial vacuoles.

Conclusion: High FDG uptake in an irradiated field may be related with radiation-induced myocardial damage resulting from microvascular damage and mitochondrial injury. An F-HFD preparation protocol used before obtaining PET/CT can improve the sensitivity of the detection of cardiotoxicity associated with radiotherapy.

No MeSH data available.


Related in: MedlinePlus

Electron transmission micrographs of myocardium.A. Abundant mitochondria packed around normal nuclei are shown in non-irradiated field (× 10000). B. Slightly dilated cristae in some mitochondria scattered around nucleus (black arrows), as well as enlarged nuclei (green arrows) were observed in irradiated myocardium (× 10000).
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Figure 6: Electron transmission micrographs of myocardium.A. Abundant mitochondria packed around normal nuclei are shown in non-irradiated field (× 10000). B. Slightly dilated cristae in some mitochondria scattered around nucleus (black arrows), as well as enlarged nuclei (green arrows) were observed in irradiated myocardium (× 10000).

Mentions: Electron transmission micrographs showed a normal myocardium with abundant mitochondria packed around normal nuclei in the non-irradiated field. However, slightly dilated cristae in some mitochondria scattered around nuclei, as well as enlarged nuclei, were observed in the irradiated myocardium (Fig. 6).


Detection of Myocardial Metabolic Abnormalities by 18F-FDG PET/CT and Corresponding Pathological Changes in Beagles with Local Heart Irradiation.

Yan R, Song J, Wu Z, Guo M, Liu J, Li J, Hao X, Li S - Korean J Radiol (2015)

Electron transmission micrographs of myocardium.A. Abundant mitochondria packed around normal nuclei are shown in non-irradiated field (× 10000). B. Slightly dilated cristae in some mitochondria scattered around nucleus (black arrows), as well as enlarged nuclei (green arrows) were observed in irradiated myocardium (× 10000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499559&req=5

Figure 6: Electron transmission micrographs of myocardium.A. Abundant mitochondria packed around normal nuclei are shown in non-irradiated field (× 10000). B. Slightly dilated cristae in some mitochondria scattered around nucleus (black arrows), as well as enlarged nuclei (green arrows) were observed in irradiated myocardium (× 10000).
Mentions: Electron transmission micrographs showed a normal myocardium with abundant mitochondria packed around normal nuclei in the non-irradiated field. However, slightly dilated cristae in some mitochondria scattered around nuclei, as well as enlarged nuclei, were observed in the irradiated myocardium (Fig. 6).

Bottom Line: High FDG uptake in irradiation field was detected in 33.3% (4/12) of 12H-F protocol and 83.3% (10/12) of F-HFD protocol in visual analysis, respectively (p = 0.031).The pathology of the irradiated myocardium showed obvious perivascular fibrosis and changes in mitochondrial vacuoles.An F-HFD preparation protocol used before obtaining PET/CT can improve the sensitivity of the detection of cardiotoxicity associated with radiotherapy.

View Article: PubMed Central - PubMed

Affiliation: Nursing College of Shanxi Medical University, Taiyuan 030001, China. ; Department of Nuclear Medicine, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT

Objective: To determine the efficacy of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) in the detection of radiation-induced myocardial damage in beagles by comparing two pre-scan preparation protocols as well as to determine the correlation between abnormal myocardial FDG uptake and pathological findings.

Materials and methods: The anterior myocardium of 12 beagles received radiotherapy locally with a single X-ray dose of 20 Gy. 18F-FDG cardiac PET/CT was performed at baseline and 3 months after radiation. Twelve beagles underwent two protocols before PET/CT: 12 hours of fasting (12H-F), 12H-F followed by a high-fat diet (F-HFD). Regions of interest were drawn on the irradiation and the non-irradiation fields to obtain their maximal standardized uptake values (SUVmax). Then the ratio of the SUV of the irradiation to the non-irradiation fields (INR) was computed. Histopathological changes were identified by light and electron microscopy.

Results: Using the 12H-F protocol, the average INRs were 1.18 ± 0.10 and 1.41 ± 0.18 before and after irradiation, respectively (p = 0.021). Using the F-HFD protocol, the average INRs were 0.99 ± 0.15 and 2.54 ± 0.43, respectively (p < 0.001). High FDG uptake in irradiation field was detected in 33.3% (4/12) of 12H-F protocol and 83.3% (10/12) of F-HFD protocol in visual analysis, respectively (p = 0.031). The pathology of the irradiated myocardium showed obvious perivascular fibrosis and changes in mitochondrial vacuoles.

Conclusion: High FDG uptake in an irradiated field may be related with radiation-induced myocardial damage resulting from microvascular damage and mitochondrial injury. An F-HFD preparation protocol used before obtaining PET/CT can improve the sensitivity of the detection of cardiotoxicity associated with radiotherapy.

No MeSH data available.


Related in: MedlinePlus