Limits...
Permeability Parameters Measured with Dynamic Contrast-Enhanced MRI: Correlation with the Extravasation of Evans Blue in a Rat Model of Transient Cerebral Ischemia.

Choi HS, Ahn SS, Shin NY, Kim J, Kim JH, Lee JE, Lee HY, Heo JH, Lee SK - Korean J Radiol (2015)

Bottom Line: Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil.All permeability parameters (K(trans), ve, and vp) showed a linear correlation with extravasation of Evans blue.Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.

ABSTRACT

Objective: The purpose of this study was to correlate permeability parameters measured with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) using a clinical 3-tesla scanner with extravasation of Evans blue in a rat model with transient cerebral ischemia.

Materials and methods: Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil. DCE-MRI was performed 12 hours, 18 hours, and 36 hours after reperfusion. Permeability parameters (K(trans), ve, and vp) from DCE-MRI were calculated. Evans blue was injected after DCE-MRI and extravasation of Evans blue was correlated as a reference with the integrity of the blood-brain barrier. Correlation analysis was performed between permeability parameters and the extravasation of Evans blue.

Results: All permeability parameters (K(trans), ve, and vp) showed a linear correlation with extravasation of Evans blue. Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001).

Conclusion: Permeability parameters obtained by DCE-MRI at 3-T are well-correlated with Evans blue extravasation, and K(trans) shows the strongest correlation among the tested parameters.

No MeSH data available.


Related in: MedlinePlus

Analysis of optical density.A. Posterior surface of sliced specimen (Bregma-1.60) was prepared for analysis of extravasation of Evans blue. ROIs were placed in right cortex (B) and basal ganglia (C) for measurement of optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4499543&req=5

Figure 2: Analysis of optical density.A. Posterior surface of sliced specimen (Bregma-1.60) was prepared for analysis of extravasation of Evans blue. ROIs were placed in right cortex (B) and basal ganglia (C) for measurement of optical density.

Mentions: The posterior surface of each section was photographed (Fig. 2A). Photographs of the posterior surface of Bregma-1.60 mm were loaded on Image J (32). The photography of the specimen was split into three color channels: red, green, and blue. Because red-channel images showed good contrast between the normal appearing brain and leakage of Evans blue, red-channel images were used for the analysis of optical density. Mean optical density was measured by ROIs in the ipsilateral cortex (Fig. 2B) and basal ganglia (Fig. 2C) along with contralateral cortex and basal ganglia. Optical density of the corpus callosum was used for normalization of those ROIs. Normalized optical densities were calculated with the following equation: (optical density of corpus callosum - optical density of ROI) / optical density of corpus callosum.


Permeability Parameters Measured with Dynamic Contrast-Enhanced MRI: Correlation with the Extravasation of Evans Blue in a Rat Model of Transient Cerebral Ischemia.

Choi HS, Ahn SS, Shin NY, Kim J, Kim JH, Lee JE, Lee HY, Heo JH, Lee SK - Korean J Radiol (2015)

Analysis of optical density.A. Posterior surface of sliced specimen (Bregma-1.60) was prepared for analysis of extravasation of Evans blue. ROIs were placed in right cortex (B) and basal ganglia (C) for measurement of optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499543&req=5

Figure 2: Analysis of optical density.A. Posterior surface of sliced specimen (Bregma-1.60) was prepared for analysis of extravasation of Evans blue. ROIs were placed in right cortex (B) and basal ganglia (C) for measurement of optical density.
Mentions: The posterior surface of each section was photographed (Fig. 2A). Photographs of the posterior surface of Bregma-1.60 mm were loaded on Image J (32). The photography of the specimen was split into three color channels: red, green, and blue. Because red-channel images showed good contrast between the normal appearing brain and leakage of Evans blue, red-channel images were used for the analysis of optical density. Mean optical density was measured by ROIs in the ipsilateral cortex (Fig. 2B) and basal ganglia (Fig. 2C) along with contralateral cortex and basal ganglia. Optical density of the corpus callosum was used for normalization of those ROIs. Normalized optical densities were calculated with the following equation: (optical density of corpus callosum - optical density of ROI) / optical density of corpus callosum.

Bottom Line: Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil.All permeability parameters (K(trans), ve, and vp) showed a linear correlation with extravasation of Evans blue.Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.

ABSTRACT

Objective: The purpose of this study was to correlate permeability parameters measured with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) using a clinical 3-tesla scanner with extravasation of Evans blue in a rat model with transient cerebral ischemia.

Materials and methods: Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil. DCE-MRI was performed 12 hours, 18 hours, and 36 hours after reperfusion. Permeability parameters (K(trans), ve, and vp) from DCE-MRI were calculated. Evans blue was injected after DCE-MRI and extravasation of Evans blue was correlated as a reference with the integrity of the blood-brain barrier. Correlation analysis was performed between permeability parameters and the extravasation of Evans blue.

Results: All permeability parameters (K(trans), ve, and vp) showed a linear correlation with extravasation of Evans blue. Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001).

Conclusion: Permeability parameters obtained by DCE-MRI at 3-T are well-correlated with Evans blue extravasation, and K(trans) shows the strongest correlation among the tested parameters.

No MeSH data available.


Related in: MedlinePlus