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Combined Radiofrequency Ablation and Double Anti-Angiogenic Protein Therapy to Increase Coagulation Efficacy: An Experimental Study in a Murine Renal Carcinoma Model.

Jun HY, Ryu JH, Byun SJ, Jeong CW, Kim TH, Lee YH, Yoon KH - Korean J Radiol (2015)

Bottom Line: Immediately after RFA, the size of coagulation necrosis and mitochondrial enzyme activity were compared between the groups using analysis of variance (ANOVA) and post hoc test.The contrast enhancement ratio for tumor vascularization on CEUS was significantly lower in the DAAP treated group than in DAAP-untreated group (30.2 ± 9.9% vs. 77.4 ± 17.3%; p = 0.021).Our results suggest the potential value of combining RFA with anti-angiogenic therapy.

View Article: PubMed Central - PubMed

Affiliation: Imaging Science Research Center, Wonkwang University School of Medicine, Iksan 570-711, Korea.

ABSTRACT

Objective: To evaluate whether suppression of tumor microvasculature by double anti-angiogenic protein (DAAP) treatment could increase the extent of radiofrequency ablation (RFA)-induced coagulation in a murine renal cell carcinoma model.

Materials and methods: Renal cell carcinoma cell lines were implanted subcutaneously into 10 nude mice. Four mice received adenoviral DAAP treatment and 6 mice received sterile 0.9% saline solution as DAAP-untreated group. The effect of DAAP was evaluated according to the vascularity by contrast-enhanced ultrasound (CEUS) using microbubbles. Four DAAP-treated mice and 4 DAAP-untreated mice were then treated with RFA, resulting in 3 groups: no-therapy (n = 2), RFA only (n = 4), and RFA combined with DAAP treatment (n = 4). Immediately after RFA, the size of coagulation necrosis and mitochondrial enzyme activity were compared between the groups using analysis of variance (ANOVA) and post hoc test.

Results: The contrast enhancement ratio for tumor vascularization on CEUS was significantly lower in the DAAP treated group than in DAAP-untreated group (30.2 ± 9.9% vs. 77.4 ± 17.3%; p = 0.021). After RFA, the mean coagulation diameter was 0 mm for no-therapy group, 6.7 ± 0.7 mm for the RFA only group and 8.5 ± 0.4 mm for the RFA with DAAP group (ANOVA, p < 0.001). The area of viable mitochondria within the tumor was 27.9 ± 3.9% in no-therapy group, 10.3 ± 4.5% in the RFA only group, and 2.1 ± 0.7% in the RFA with DAAP group (ANOVA, p < 0.001).

Conclusion: Our results suggest the potential value of combining RFA with anti-angiogenic therapy.

No MeSH data available.


Related in: MedlinePlus

Graph demonstrating contrast enhancement ratios (CER).CER after injection of microbubbles was significantly different between untreated tumor and double anti-angiogenic protein (DAAP)-treated tumor. *p < 0.05.
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Figure 2: Graph demonstrating contrast enhancement ratios (CER).CER after injection of microbubbles was significantly different between untreated tumor and double anti-angiogenic protein (DAAP)-treated tumor. *p < 0.05.

Mentions: The mean tumor area measured at the largest cross-section by ultrasound before RFA was 77.1 ± 20.2 mm2 and 69.5 ± 28.3 mm2 in DAAP-untreated group and DAAP-treated group, respectively, at 7 days after administration DAAP, with no significant difference between groups (Table 1). CEUS images captured at 1 minute after intravenous injection of microbubbles showed lower signal intensity in DAAP-treated mice than in DAAP-untreated mice (Fig. 1). The CER for tumor vascularization on CEUS images were significantly lower in DAAP-treated group (30.2 ± 9.9%) than in DAAP-untreated group (77.4 ± 17.3%; p = 0.021) (Fig. 2).


Combined Radiofrequency Ablation and Double Anti-Angiogenic Protein Therapy to Increase Coagulation Efficacy: An Experimental Study in a Murine Renal Carcinoma Model.

Jun HY, Ryu JH, Byun SJ, Jeong CW, Kim TH, Lee YH, Yoon KH - Korean J Radiol (2015)

Graph demonstrating contrast enhancement ratios (CER).CER after injection of microbubbles was significantly different between untreated tumor and double anti-angiogenic protein (DAAP)-treated tumor. *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499541&req=5

Figure 2: Graph demonstrating contrast enhancement ratios (CER).CER after injection of microbubbles was significantly different between untreated tumor and double anti-angiogenic protein (DAAP)-treated tumor. *p < 0.05.
Mentions: The mean tumor area measured at the largest cross-section by ultrasound before RFA was 77.1 ± 20.2 mm2 and 69.5 ± 28.3 mm2 in DAAP-untreated group and DAAP-treated group, respectively, at 7 days after administration DAAP, with no significant difference between groups (Table 1). CEUS images captured at 1 minute after intravenous injection of microbubbles showed lower signal intensity in DAAP-treated mice than in DAAP-untreated mice (Fig. 1). The CER for tumor vascularization on CEUS images were significantly lower in DAAP-treated group (30.2 ± 9.9%) than in DAAP-untreated group (77.4 ± 17.3%; p = 0.021) (Fig. 2).

Bottom Line: Immediately after RFA, the size of coagulation necrosis and mitochondrial enzyme activity were compared between the groups using analysis of variance (ANOVA) and post hoc test.The contrast enhancement ratio for tumor vascularization on CEUS was significantly lower in the DAAP treated group than in DAAP-untreated group (30.2 ± 9.9% vs. 77.4 ± 17.3%; p = 0.021).Our results suggest the potential value of combining RFA with anti-angiogenic therapy.

View Article: PubMed Central - PubMed

Affiliation: Imaging Science Research Center, Wonkwang University School of Medicine, Iksan 570-711, Korea.

ABSTRACT

Objective: To evaluate whether suppression of tumor microvasculature by double anti-angiogenic protein (DAAP) treatment could increase the extent of radiofrequency ablation (RFA)-induced coagulation in a murine renal cell carcinoma model.

Materials and methods: Renal cell carcinoma cell lines were implanted subcutaneously into 10 nude mice. Four mice received adenoviral DAAP treatment and 6 mice received sterile 0.9% saline solution as DAAP-untreated group. The effect of DAAP was evaluated according to the vascularity by contrast-enhanced ultrasound (CEUS) using microbubbles. Four DAAP-treated mice and 4 DAAP-untreated mice were then treated with RFA, resulting in 3 groups: no-therapy (n = 2), RFA only (n = 4), and RFA combined with DAAP treatment (n = 4). Immediately after RFA, the size of coagulation necrosis and mitochondrial enzyme activity were compared between the groups using analysis of variance (ANOVA) and post hoc test.

Results: The contrast enhancement ratio for tumor vascularization on CEUS was significantly lower in the DAAP treated group than in DAAP-untreated group (30.2 ± 9.9% vs. 77.4 ± 17.3%; p = 0.021). After RFA, the mean coagulation diameter was 0 mm for no-therapy group, 6.7 ± 0.7 mm for the RFA only group and 8.5 ± 0.4 mm for the RFA with DAAP group (ANOVA, p < 0.001). The area of viable mitochondria within the tumor was 27.9 ± 3.9% in no-therapy group, 10.3 ± 4.5% in the RFA only group, and 2.1 ± 0.7% in the RFA with DAAP group (ANOVA, p < 0.001).

Conclusion: Our results suggest the potential value of combining RFA with anti-angiogenic therapy.

No MeSH data available.


Related in: MedlinePlus