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Integrated Treatment of Aqueous Extract of Solanum nigrum-Potentiated Cisplatin- and Doxorubicin-Induced Cytotoxicity in Human Hepatocellular Carcinoma Cells.

Wang CK, Lin YF, Tai CJ, Wang CW, Chang YJ, Choong CY, Lin CS, Tai CJ, Chang CC - Evid Based Complement Alternat Med (2015)

Bottom Line: Therefore, this study examined the tumor-suppression efficiency of AE-SN integrated with a standard chemotherapeutic drug, namely, cisplatin or doxorubicin, in human HCC cells, namely, Hep3B and HepJ5.The results suggested that the integrated treatment with AE-SN-potentiated cisplatin and doxorubicin induced cytotoxicity through the cleavage of caspase-7 and accumulation of microtubule-associated protein-1 light chain-3 A/1B II (LC-3 A/B II), which were associated with apoptotic and autophagic cell death, respectively, in both the Hep3B and HepJ5 cells.In conclusion, AE-SN can potentially be used in novel integrated chemotherapy with cisplatin or doxorubicin to treat HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan ; Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan ; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan ; Department of Chinese Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan.

ABSTRACT
Chemotherapy is the main approach for treating advanced and recurrent hepatocellular carcinoma (HCC), but the clinical performance of chemotherapy is limited by a relatively low response rate, drug resistance, and adverse effects that severely affect the quality of life of patients. The aqueous extract of Solanum nigrum (AE-SN) is a crucial ingredient in some traditional Chinese medicine (TCM) formulas for treating cancer patients and exhibits antitumor effects in human HCC cells. Therefore, this study examined the tumor-suppression efficiency of AE-SN integrated with a standard chemotherapeutic drug, namely, cisplatin or doxorubicin, in human HCC cells, namely, Hep3B and HepJ5. The results suggested that the integrated treatment with AE-SN-potentiated cisplatin and doxorubicin induced cytotoxicity through the cleavage of caspase-7 and accumulation of microtubule-associated protein-1 light chain-3 A/1B II (LC-3 A/B II), which were associated with apoptotic and autophagic cell death, respectively, in both the Hep3B and HepJ5 cells. In conclusion, AE-SN can potentially be used in novel integrated chemotherapy with cisplatin or doxorubicin to treat HCC patients.

No MeSH data available.


Related in: MedlinePlus

Extracted ion chromatography of solamargine. (a) The parent ion of purified solamargine was fragmented into two daughter ions (blue and red peaks). (b) Fragmented ions presented in AE-SN.
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fig1: Extracted ion chromatography of solamargine. (a) The parent ion of purified solamargine was fragmented into two daughter ions (blue and red peaks). (b) Fragmented ions presented in AE-SN.

Mentions: Solamargine is considered as a crucial antitumor component that existed in Solanum nigrum and can be a marker component for AE-SN [17]. The concentration of solamargine was therefore determined in the AE-SN stock solution by using liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Before analysis, 100 μL AE-SN stock solution was mixed with 900 μL mixture of water : methanol (30 : 70, v/v). LC-MS/MS analysis was performed in the mobile phase of HPLC-grade acetonitrile (ACN) and deionized water with a Syncronis C18 column (Thermo Scientific, MA, USA). The flow rate is 0.5 mL/min with splitted 0.25 mL/min to mass. The ionization mode of the mass spectrometry condition was set as electrospray/positive ionization, and the mass scanning mode was multiple reaction monitor (MRM). The parent ion of purified solamargine is 867.5 m/z and fragmented into two daughter ions: 722.5 and 396.4 m/z, respectively (Figure 1(a)). These daughter ions were used to determine the solamargine concentration in AE-SN (Figure 1(b)). The concentration of solamargine in AE-SN stock solution was 77 μg/mL by using MRM analysis.


Integrated Treatment of Aqueous Extract of Solanum nigrum-Potentiated Cisplatin- and Doxorubicin-Induced Cytotoxicity in Human Hepatocellular Carcinoma Cells.

Wang CK, Lin YF, Tai CJ, Wang CW, Chang YJ, Choong CY, Lin CS, Tai CJ, Chang CC - Evid Based Complement Alternat Med (2015)

Extracted ion chromatography of solamargine. (a) The parent ion of purified solamargine was fragmented into two daughter ions (blue and red peaks). (b) Fragmented ions presented in AE-SN.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499398&req=5

fig1: Extracted ion chromatography of solamargine. (a) The parent ion of purified solamargine was fragmented into two daughter ions (blue and red peaks). (b) Fragmented ions presented in AE-SN.
Mentions: Solamargine is considered as a crucial antitumor component that existed in Solanum nigrum and can be a marker component for AE-SN [17]. The concentration of solamargine was therefore determined in the AE-SN stock solution by using liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Before analysis, 100 μL AE-SN stock solution was mixed with 900 μL mixture of water : methanol (30 : 70, v/v). LC-MS/MS analysis was performed in the mobile phase of HPLC-grade acetonitrile (ACN) and deionized water with a Syncronis C18 column (Thermo Scientific, MA, USA). The flow rate is 0.5 mL/min with splitted 0.25 mL/min to mass. The ionization mode of the mass spectrometry condition was set as electrospray/positive ionization, and the mass scanning mode was multiple reaction monitor (MRM). The parent ion of purified solamargine is 867.5 m/z and fragmented into two daughter ions: 722.5 and 396.4 m/z, respectively (Figure 1(a)). These daughter ions were used to determine the solamargine concentration in AE-SN (Figure 1(b)). The concentration of solamargine in AE-SN stock solution was 77 μg/mL by using MRM analysis.

Bottom Line: Therefore, this study examined the tumor-suppression efficiency of AE-SN integrated with a standard chemotherapeutic drug, namely, cisplatin or doxorubicin, in human HCC cells, namely, Hep3B and HepJ5.The results suggested that the integrated treatment with AE-SN-potentiated cisplatin and doxorubicin induced cytotoxicity through the cleavage of caspase-7 and accumulation of microtubule-associated protein-1 light chain-3 A/1B II (LC-3 A/B II), which were associated with apoptotic and autophagic cell death, respectively, in both the Hep3B and HepJ5 cells.In conclusion, AE-SN can potentially be used in novel integrated chemotherapy with cisplatin or doxorubicin to treat HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan ; Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan ; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan ; Department of Chinese Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan.

ABSTRACT
Chemotherapy is the main approach for treating advanced and recurrent hepatocellular carcinoma (HCC), but the clinical performance of chemotherapy is limited by a relatively low response rate, drug resistance, and adverse effects that severely affect the quality of life of patients. The aqueous extract of Solanum nigrum (AE-SN) is a crucial ingredient in some traditional Chinese medicine (TCM) formulas for treating cancer patients and exhibits antitumor effects in human HCC cells. Therefore, this study examined the tumor-suppression efficiency of AE-SN integrated with a standard chemotherapeutic drug, namely, cisplatin or doxorubicin, in human HCC cells, namely, Hep3B and HepJ5. The results suggested that the integrated treatment with AE-SN-potentiated cisplatin and doxorubicin induced cytotoxicity through the cleavage of caspase-7 and accumulation of microtubule-associated protein-1 light chain-3 A/1B II (LC-3 A/B II), which were associated with apoptotic and autophagic cell death, respectively, in both the Hep3B and HepJ5 cells. In conclusion, AE-SN can potentially be used in novel integrated chemotherapy with cisplatin or doxorubicin to treat HCC patients.

No MeSH data available.


Related in: MedlinePlus