Limits...
MicroRNA Clusters in the Adult Mouse Heart: Age-Associated Changes.

Zhang X, Azhar G, Williams ED, Rogers SC, Wei JY - Biomed Res Int (2015)

Bottom Line: Since a microRNA cluster has from two to dozens of microRNAs, the expression of a microRNA cluster could have a substantial impact on its target genes.Multiple microRNAs derived from the three clusters potentially target various protein components of the cdc42-SRF signaling pathway, which regulates cytoskeleton dynamics associated with cardiac structure and function.The data indicate that aging impacted the expression of both guide and passenger strands of the microRNA clusters; nutrient stress also affected the expression of the three microRNA clusters.

View Article: PubMed Central - PubMed

Affiliation: Reynolds Institute on Aging and Department of Geriatrics, University of Arkansas for Medical Science, 4301 West Markham Street, No. 748, Little Rock, AR 72205, USA ; Geriatric Research Education and Clinical Center, Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA.

ABSTRACT
The microRNAs and microRNA clusters have been implicated in normal cardiac development and also disease, including cardiac hypertrophy, cardiomyopathy, heart failure, and arrhythmias. Since a microRNA cluster has from two to dozens of microRNAs, the expression of a microRNA cluster could have a substantial impact on its target genes. In the present study, the configuration and distribution of microRNA clusters in the mouse genome were examined at various inter-microRNA distances. Three important microRNA clusters that are significantly impacted during adult cardiac aging, the miR-17-92, miR-106a-363, and miR-106b-25, were also examined in terms of their genomic location, RNA transcript character, sequence homology, and their relationship with the corresponding microRNA families. Multiple microRNAs derived from the three clusters potentially target various protein components of the cdc42-SRF signaling pathway, which regulates cytoskeleton dynamics associated with cardiac structure and function. The data indicate that aging impacted the expression of both guide and passenger strands of the microRNA clusters; nutrient stress also affected the expression of the three microRNA clusters. The miR-17-92, miR-106a-363, and miR-106b-25 clusters are likely to impact the Cdc42-SRF signaling pathway and thereby affect cardiac morphology and function during pathological conditions and the aging process.

No MeSH data available.


Related in: MedlinePlus

Genomic loci of miR-17-92, miR-106a, and miR-106b-25 clusters. The miR-17-92 cluster spans approximately 825 bp on chromosome 14. The miR-106a-363 cluster spans approximately 736 bp on chromosome X. The miR-106b-25 cluster spans 333 bp on chromosome 5.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4499379&req=5

fig2: Genomic loci of miR-17-92, miR-106a, and miR-106b-25 clusters. The miR-17-92 cluster spans approximately 825 bp on chromosome 14. The miR-106a-363 cluster spans approximately 736 bp on chromosome X. The miR-106b-25 cluster spans 333 bp on chromosome 5.

Mentions: In the mouse genome, the miR-17-92 cluster is located on chromosome 14, the miR-106a-363 cluster is on chromosome X, and miR-106b-25 cluster is on chromosome 5 (Figure 2). The gene loci of all 3 clusters span less than 1 Kb, indicating that the miRNAs in each cluster are likely to be transcribed within one transcript.


MicroRNA Clusters in the Adult Mouse Heart: Age-Associated Changes.

Zhang X, Azhar G, Williams ED, Rogers SC, Wei JY - Biomed Res Int (2015)

Genomic loci of miR-17-92, miR-106a, and miR-106b-25 clusters. The miR-17-92 cluster spans approximately 825 bp on chromosome 14. The miR-106a-363 cluster spans approximately 736 bp on chromosome X. The miR-106b-25 cluster spans 333 bp on chromosome 5.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499379&req=5

fig2: Genomic loci of miR-17-92, miR-106a, and miR-106b-25 clusters. The miR-17-92 cluster spans approximately 825 bp on chromosome 14. The miR-106a-363 cluster spans approximately 736 bp on chromosome X. The miR-106b-25 cluster spans 333 bp on chromosome 5.
Mentions: In the mouse genome, the miR-17-92 cluster is located on chromosome 14, the miR-106a-363 cluster is on chromosome X, and miR-106b-25 cluster is on chromosome 5 (Figure 2). The gene loci of all 3 clusters span less than 1 Kb, indicating that the miRNAs in each cluster are likely to be transcribed within one transcript.

Bottom Line: Since a microRNA cluster has from two to dozens of microRNAs, the expression of a microRNA cluster could have a substantial impact on its target genes.Multiple microRNAs derived from the three clusters potentially target various protein components of the cdc42-SRF signaling pathway, which regulates cytoskeleton dynamics associated with cardiac structure and function.The data indicate that aging impacted the expression of both guide and passenger strands of the microRNA clusters; nutrient stress also affected the expression of the three microRNA clusters.

View Article: PubMed Central - PubMed

Affiliation: Reynolds Institute on Aging and Department of Geriatrics, University of Arkansas for Medical Science, 4301 West Markham Street, No. 748, Little Rock, AR 72205, USA ; Geriatric Research Education and Clinical Center, Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA.

ABSTRACT
The microRNAs and microRNA clusters have been implicated in normal cardiac development and also disease, including cardiac hypertrophy, cardiomyopathy, heart failure, and arrhythmias. Since a microRNA cluster has from two to dozens of microRNAs, the expression of a microRNA cluster could have a substantial impact on its target genes. In the present study, the configuration and distribution of microRNA clusters in the mouse genome were examined at various inter-microRNA distances. Three important microRNA clusters that are significantly impacted during adult cardiac aging, the miR-17-92, miR-106a-363, and miR-106b-25, were also examined in terms of their genomic location, RNA transcript character, sequence homology, and their relationship with the corresponding microRNA families. Multiple microRNAs derived from the three clusters potentially target various protein components of the cdc42-SRF signaling pathway, which regulates cytoskeleton dynamics associated with cardiac structure and function. The data indicate that aging impacted the expression of both guide and passenger strands of the microRNA clusters; nutrient stress also affected the expression of the three microRNA clusters. The miR-17-92, miR-106a-363, and miR-106b-25 clusters are likely to impact the Cdc42-SRF signaling pathway and thereby affect cardiac morphology and function during pathological conditions and the aging process.

No MeSH data available.


Related in: MedlinePlus