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Increased expression of ARF GTPases in prostate cancer tissue.

Morgan C, Lewis PD, Hopkins L, Burnell S, Kynaston H, Doak SH - Springerplus (2015)

Bottom Line: ARFs are a family of Ras-related GTP binding proteins, ARF6, in particular, is implicated in cancer invasion and metastasis.However, the role of ARF proteins in prostate cancer have yet to be investigated.Antibody staining was significantly over-expressed in prostate cancer patient samples compared to normal patient tissue and a trend towards increased staining intensity in cancer samples with Gleason scores of 8 and above (metastatic disease).

View Article: PubMed Central - PubMed

Affiliation: Institute of Life Science, Swansea University, Swansea, UK.

ABSTRACT

Purpose: ARFs are a family of Ras-related GTP binding proteins, ARF6, in particular, is implicated in cancer invasion and metastasis. However, the role of ARF proteins in prostate cancer have yet to be investigated.

Methods: Immunohistochemical staining for ARF6 was performed on a prostate cancer tissue microarray with patient matched normal specimens.

Results: Antibody staining was significantly over-expressed in prostate cancer patient samples compared to normal patient tissue and a trend towards increased staining intensity in cancer samples with Gleason scores of 8 and above (metastatic disease).

Conclusion: Due to high homology between members of the ARF family we could not determine if ARF 6 was the only ARF over-expressed in the prostate cancer samples. However, we are the first to show that ARF-GTPases are over expressed in prostate cancer which provides further insight into the molecular biology of prostate cancer.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical detection of ARF protein expression in a normal prostate tissue and b prostate cancer tissue scored as Gleason grade 9.
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Fig1: Immunohistochemical detection of ARF protein expression in a normal prostate tissue and b prostate cancer tissue scored as Gleason grade 9.

Mentions: Immunohistochemical analysis using a commercial antibody to ARF6 showed a significant increase in the observed sum of staining intensity (protein expression) and distribution between cancer and matched normal samples (P = 0.047, Figure 1). In normal tissue 73% (N = 22) of the samples showed a higher proportion of weak staining intensity relative to moderate staining (27%, N = 8) but there were equal numbers of cases showing both weak (N = 15) and moderate staining intensity (N = 15) in tumour tissue (Figure 2). Thus, there was an observed higher level of moderate staining intensity in tumours relative to normal tissue. Based on the hypothesis of increased staining/protein expression in tumour samples, the difference was statistically significant (P = 0.044). However, there was no significant difference in staining distribution in tumour (score = 2, N = 7; score = 3, N = 23) relative to normal tissue (score = 2, N = 8; score = 3, N = 22) (P = 0.360). Thus, 77 and 73% of tumour and normal samples respectively showed ARF6 staining in >60% of the tissue sample.Figure 1


Increased expression of ARF GTPases in prostate cancer tissue.

Morgan C, Lewis PD, Hopkins L, Burnell S, Kynaston H, Doak SH - Springerplus (2015)

Immunohistochemical detection of ARF protein expression in a normal prostate tissue and b prostate cancer tissue scored as Gleason grade 9.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499334&req=5

Fig1: Immunohistochemical detection of ARF protein expression in a normal prostate tissue and b prostate cancer tissue scored as Gleason grade 9.
Mentions: Immunohistochemical analysis using a commercial antibody to ARF6 showed a significant increase in the observed sum of staining intensity (protein expression) and distribution between cancer and matched normal samples (P = 0.047, Figure 1). In normal tissue 73% (N = 22) of the samples showed a higher proportion of weak staining intensity relative to moderate staining (27%, N = 8) but there were equal numbers of cases showing both weak (N = 15) and moderate staining intensity (N = 15) in tumour tissue (Figure 2). Thus, there was an observed higher level of moderate staining intensity in tumours relative to normal tissue. Based on the hypothesis of increased staining/protein expression in tumour samples, the difference was statistically significant (P = 0.044). However, there was no significant difference in staining distribution in tumour (score = 2, N = 7; score = 3, N = 23) relative to normal tissue (score = 2, N = 8; score = 3, N = 22) (P = 0.360). Thus, 77 and 73% of tumour and normal samples respectively showed ARF6 staining in >60% of the tissue sample.Figure 1

Bottom Line: ARFs are a family of Ras-related GTP binding proteins, ARF6, in particular, is implicated in cancer invasion and metastasis.However, the role of ARF proteins in prostate cancer have yet to be investigated.Antibody staining was significantly over-expressed in prostate cancer patient samples compared to normal patient tissue and a trend towards increased staining intensity in cancer samples with Gleason scores of 8 and above (metastatic disease).

View Article: PubMed Central - PubMed

Affiliation: Institute of Life Science, Swansea University, Swansea, UK.

ABSTRACT

Purpose: ARFs are a family of Ras-related GTP binding proteins, ARF6, in particular, is implicated in cancer invasion and metastasis. However, the role of ARF proteins in prostate cancer have yet to be investigated.

Methods: Immunohistochemical staining for ARF6 was performed on a prostate cancer tissue microarray with patient matched normal specimens.

Results: Antibody staining was significantly over-expressed in prostate cancer patient samples compared to normal patient tissue and a trend towards increased staining intensity in cancer samples with Gleason scores of 8 and above (metastatic disease).

Conclusion: Due to high homology between members of the ARF family we could not determine if ARF 6 was the only ARF over-expressed in the prostate cancer samples. However, we are the first to show that ARF-GTPases are over expressed in prostate cancer which provides further insight into the molecular biology of prostate cancer.

No MeSH data available.


Related in: MedlinePlus