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Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.

Kurnik K, Treder K, Skorupa-Kłaput M, Tretyn A, Tyburski J - Water Air Soil Pollut (2015)

Bottom Line: The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations.The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range.Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions.

View Article: PubMed Central - PubMed

Affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.

ABSTRACT

Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

No MeSH data available.


Related in: MedlinePlus

The effect of shaking rate on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were conducted at different shaking rates. The percentage of phenol removed was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05
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Fig7: The effect of shaking rate on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were conducted at different shaking rates. The percentage of phenol removed was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05

Mentions: Most studies testing the ability of peroxidases to detoxify phenol solutions used shaking rates from 50 to 200 rpm to achieve optimal results (González et al. 2008, 2013; Paisio et al. 2010; Gholizadeh et al. 2013; Asadgol et al. 2014; Kalaiarasan and Palvannan 2014; Malani et al. 2013; Abdallah 2013; Angelini et al. 2014). However, to the best of our knowledge, assessing the effect of various shaking rates on the efficiency of phenol removal by peroxidase has not yet been tested as a distinct experiment. To assess the effect of shaking rate on phenol removal by potato pulp, the reaction mixtures subjected to shaking rates from 50 to 200 rpm were compared to the control, which was mixed after the reaction mixture was composed, and then left unshaken. The differences in the removal efficiency between the shaken samples compared to the control were the most obvious for the 1 mM phenol solutions. The variants that were not shaken displayed phenol removal efficiencies of 70 %, whereas shaking the samples enabled almost 100 % phenol removal within the entire range of shaking intensities (Fig. 7a, b). In the case of 2 mM phenol, the unshaken controls displayed removal efficiencies of 85 %, significantly lower than in the agitated variants. Agitating samples at 50 rpm increased phenol removal up to 98 %. The other variants also displayed removal efficiencies close to 100 % (Fig. 7c, d). After 2 and 3 h of reaction, there were no statistically significant differences in phenol removal efficiencies at shaking rates from 50 to 200 rpm. Slightly lower removal efficiencies were detected for reaction mixtures with 3 mM phenol. The unshaken controls displayed removal efficiencies of 87 %, and the efficiencies did not significantly change over the agitation rates applied, except the samples subjected to 150 rpm, after 2-h incubation, rising to over 90 % phenol removal (Fig. 7e, f).Fig. 7


Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.

Kurnik K, Treder K, Skorupa-Kłaput M, Tretyn A, Tyburski J - Water Air Soil Pollut (2015)

The effect of shaking rate on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were conducted at different shaking rates. The percentage of phenol removed was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499105&req=5

Fig7: The effect of shaking rate on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were conducted at different shaking rates. The percentage of phenol removed was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05
Mentions: Most studies testing the ability of peroxidases to detoxify phenol solutions used shaking rates from 50 to 200 rpm to achieve optimal results (González et al. 2008, 2013; Paisio et al. 2010; Gholizadeh et al. 2013; Asadgol et al. 2014; Kalaiarasan and Palvannan 2014; Malani et al. 2013; Abdallah 2013; Angelini et al. 2014). However, to the best of our knowledge, assessing the effect of various shaking rates on the efficiency of phenol removal by peroxidase has not yet been tested as a distinct experiment. To assess the effect of shaking rate on phenol removal by potato pulp, the reaction mixtures subjected to shaking rates from 50 to 200 rpm were compared to the control, which was mixed after the reaction mixture was composed, and then left unshaken. The differences in the removal efficiency between the shaken samples compared to the control were the most obvious for the 1 mM phenol solutions. The variants that were not shaken displayed phenol removal efficiencies of 70 %, whereas shaking the samples enabled almost 100 % phenol removal within the entire range of shaking intensities (Fig. 7a, b). In the case of 2 mM phenol, the unshaken controls displayed removal efficiencies of 85 %, significantly lower than in the agitated variants. Agitating samples at 50 rpm increased phenol removal up to 98 %. The other variants also displayed removal efficiencies close to 100 % (Fig. 7c, d). After 2 and 3 h of reaction, there were no statistically significant differences in phenol removal efficiencies at shaking rates from 50 to 200 rpm. Slightly lower removal efficiencies were detected for reaction mixtures with 3 mM phenol. The unshaken controls displayed removal efficiencies of 87 %, and the efficiencies did not significantly change over the agitation rates applied, except the samples subjected to 150 rpm, after 2-h incubation, rising to over 90 % phenol removal (Fig. 7e, f).Fig. 7

Bottom Line: The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations.The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range.Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions.

View Article: PubMed Central - PubMed

Affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.

ABSTRACT

Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

No MeSH data available.


Related in: MedlinePlus