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Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.

Kurnik K, Treder K, Skorupa-Kłaput M, Tretyn A, Tyburski J - Water Air Soil Pollut (2015)

Bottom Line: The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations.The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range.Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions.

View Article: PubMed Central - PubMed

Affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.

ABSTRACT

Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

No MeSH data available.


Related in: MedlinePlus

The effect of temperature on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were incubated at different temperatures. The percentage of phenol removal was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05
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Fig6: The effect of temperature on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were incubated at different temperatures. The percentage of phenol removal was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05

Mentions: Peroxidases usually have a wide temperature range within which their activity is maintained (González et al. 2006). Here, we studied the efficiency of phenol removal at temperatures from 10 to 60 °C. We observed that phenol removal displayed high efficiency within the tested temperature range. In the presence of 1 mM phenol, the removal efficiency did not drop below 95 %, even at the highest temperature tested (Fig. 6a, b). Similarly, the removal efficiency for 2 mM phenol was 97 % at 10 °C after 2 h of incubation and did not significantly change over the entire temperature range (Fig. 6c, d). The most distinct differences in phenol removal efficiencies at different temperatures were observed if the reaction was carried out in the presence of 3 mM phenol. In that case, at both 10 and 60 °C, we observed a slight inhibitory effect on the reaction; however, the removal efficiencies were still over 88 % and we detected no significant differences within the tested temperature range (Fig. 5e, f). For comparison, Kalaiarasan and Palvannan (2014) reported that native HRP lost its activity at 40 °C. However, the enzyme’s capacity for phenol removal was maintained at higher temperatures (65 °C) if dextran and sodium alginate were added to the reaction mixture. By contrast, González et al. (2006) observed the highest phenol removal efficiency at 40 and 60 °C, using tomato hairy roots.Fig. 6


Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.

Kurnik K, Treder K, Skorupa-Kłaput M, Tretyn A, Tyburski J - Water Air Soil Pollut (2015)

The effect of temperature on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were incubated at different temperatures. The percentage of phenol removal was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4499105&req=5

Fig6: The effect of temperature on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2. The assays were incubated at different temperatures. The percentage of phenol removal was determined after 2 (a, c, e) or 3 (b, d, f) hours of incubation. The assay solutions were initially supplemented with 1 (a, b), 2 (c, d), or 3 (e, f) mM phenol. Different letters denote significant differences at p ≤ 0.05
Mentions: Peroxidases usually have a wide temperature range within which their activity is maintained (González et al. 2006). Here, we studied the efficiency of phenol removal at temperatures from 10 to 60 °C. We observed that phenol removal displayed high efficiency within the tested temperature range. In the presence of 1 mM phenol, the removal efficiency did not drop below 95 %, even at the highest temperature tested (Fig. 6a, b). Similarly, the removal efficiency for 2 mM phenol was 97 % at 10 °C after 2 h of incubation and did not significantly change over the entire temperature range (Fig. 6c, d). The most distinct differences in phenol removal efficiencies at different temperatures were observed if the reaction was carried out in the presence of 3 mM phenol. In that case, at both 10 and 60 °C, we observed a slight inhibitory effect on the reaction; however, the removal efficiencies were still over 88 % and we detected no significant differences within the tested temperature range (Fig. 5e, f). For comparison, Kalaiarasan and Palvannan (2014) reported that native HRP lost its activity at 40 °C. However, the enzyme’s capacity for phenol removal was maintained at higher temperatures (65 °C) if dextran and sodium alginate were added to the reaction mixture. By contrast, González et al. (2006) observed the highest phenol removal efficiency at 40 and 60 °C, using tomato hairy roots.Fig. 6

Bottom Line: The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations.The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range.Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions.

View Article: PubMed Central - PubMed

Affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.

ABSTRACT

Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

No MeSH data available.


Related in: MedlinePlus