Limits...
Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.

Kurnik K, Treder K, Skorupa-Kłaput M, Tretyn A, Tyburski J - Water Air Soil Pollut (2015)

Bottom Line: The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations.The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range.Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions.

View Article: PubMed Central - PubMed

Affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.

ABSTRACT

Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

No MeSH data available.


Related in: MedlinePlus

The effect of pH on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2 and in corresponding control mixtures containing phenol and potato pulp but not H2O2. The assays were carried on at pH 2.0 (a), pH 4.0 (b), pH 6.0 (c), pH 7.3 (tap water) (d), pH 8.0 (e), or pH 10.0 (f). The reactions were incubated for 2 (black bars) or 3 (gray bars) hours. Different letters denote significant differences at p ≤ 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4499105&req=5

Fig5: The effect of pH on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2 and in corresponding control mixtures containing phenol and potato pulp but not H2O2. The assays were carried on at pH 2.0 (a), pH 4.0 (b), pH 6.0 (c), pH 7.3 (tap water) (d), pH 8.0 (e), or pH 10.0 (f). The reactions were incubated for 2 (black bars) or 3 (gray bars) hours. Different letters denote significant differences at p ≤ 0.05

Mentions: The pH range tested was from pH 2.0 to 10.0 for initial phenol concentrations from 1 to 4 mM (Fig. 5). The efficiency of phenol removal was measured after 2 or 3 h of incubation. Each time, H2O2-free controls were run along with the reactions to monitor peroxidase-independent changes in phenol concentration. At pH 2.0, the phenol was either not removed from the solution or the removal efficiency was low (Fig. 5a).Fig. 5


Removal of Phenol from Synthetic and Industrial Wastewater by Potato Pulp Peroxidases.

Kurnik K, Treder K, Skorupa-Kłaput M, Tretyn A, Tyburski J - Water Air Soil Pollut (2015)

The effect of pH on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2 and in corresponding control mixtures containing phenol and potato pulp but not H2O2. The assays were carried on at pH 2.0 (a), pH 4.0 (b), pH 6.0 (c), pH 7.3 (tap water) (d), pH 8.0 (e), or pH 10.0 (f). The reactions were incubated for 2 (black bars) or 3 (gray bars) hours. Different letters denote significant differences at p ≤ 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499105&req=5

Fig5: The effect of pH on the efficiency of phenol (phe) removal. Efficiency of phenol removal was assessed in assay mixtures composed of phenol solution, potato pulp inoculum, and H2O2 and in corresponding control mixtures containing phenol and potato pulp but not H2O2. The assays were carried on at pH 2.0 (a), pH 4.0 (b), pH 6.0 (c), pH 7.3 (tap water) (d), pH 8.0 (e), or pH 10.0 (f). The reactions were incubated for 2 (black bars) or 3 (gray bars) hours. Different letters denote significant differences at p ≤ 0.05
Mentions: The pH range tested was from pH 2.0 to 10.0 for initial phenol concentrations from 1 to 4 mM (Fig. 5). The efficiency of phenol removal was measured after 2 or 3 h of incubation. Each time, H2O2-free controls were run along with the reactions to monitor peroxidase-independent changes in phenol concentration. At pH 2.0, the phenol was either not removed from the solution or the removal efficiency was low (Fig. 5a).Fig. 5

Bottom Line: The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations.The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range.Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions.

View Article: PubMed Central - PubMed

Affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.

ABSTRACT

Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.

No MeSH data available.


Related in: MedlinePlus