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Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap.

Rashvand Z, Heidari M, Raoofian R, Modarresi MH, Shirkoohi R - Iran. J. Public Health (2013)

Bottom Line: TGIFLX gene expression was confirmed by RT-PCR.Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P<0.01).The TGIFLX expression was confirmed by RT-PCR and fluorescent microscopy.

View Article: PubMed Central - PubMed

Affiliation: 1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.

ABSTRACT

Background: TGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains unknown. The purpose of this study was to evaluate the consequences of TGIFLX expression on prostate cancer cell lines (LNCaP).

Method: Inducible Tet-On gene expression system was used with a regulatory capability by doxycycline induction. In this system, stable LNCaP cells with TGIFLX tet-on plasmid were able to induce TGIFLX expression by doxycycline treatment. TGIFLX gene expression was confirmed by RT-PCR.

Results: Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P<0.01). Also, by using PEGFPN1 plasmid protein in this study localization was shown in nucleus. The gene was cloned in the plasmid and transfected to LNcap cells with plasmid PEGFPN1 TGIFLX and the plasmid was PEGFPN1. The TGIFLX expression was confirmed by RT-PCR and fluorescent microscopy.

Conclusion: TGIFLX expression demonstrated a tumor suppressor characterization in a prostatic cancer cell line with low grade of tumorigenicity (LNCaP). More cell lines with different level of tumorogenicity need to be investigated for further clarification of the TGIFLX gene function.

No MeSH data available.


Related in: MedlinePlus

MTT assay has shown a dramatic effect of TGIFLX expression on cell viability in TGIFLX expressing (LNX-1) cells compared with wild type (Lncap) and empty vector (LNN-1) stable cell lines (P<0.05)
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Figure 3: MTT assay has shown a dramatic effect of TGIFLX expression on cell viability in TGIFLX expressing (LNX-1) cells compared with wild type (Lncap) and empty vector (LNN-1) stable cell lines (P<0.05)

Mentions: MTT assay was investigated in 550nm absorbance. As it is shown in Fig. 3, it was 0.37, 0.41 and 0.34 for LNCaP, LNN-1 and untreated LNX-1, while this range was reduced to 0.25 after LNX-1 TGIFLX induction by doxycyclin (P<0.005). Data has shown lower metabolic activity in LNX-1 treated with doxycyclin compared with untreated cells LNN-1.


Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap.

Rashvand Z, Heidari M, Raoofian R, Modarresi MH, Shirkoohi R - Iran. J. Public Health (2013)

MTT assay has shown a dramatic effect of TGIFLX expression on cell viability in TGIFLX expressing (LNX-1) cells compared with wild type (Lncap) and empty vector (LNN-1) stable cell lines (P<0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499065&req=5

Figure 3: MTT assay has shown a dramatic effect of TGIFLX expression on cell viability in TGIFLX expressing (LNX-1) cells compared with wild type (Lncap) and empty vector (LNN-1) stable cell lines (P<0.05)
Mentions: MTT assay was investigated in 550nm absorbance. As it is shown in Fig. 3, it was 0.37, 0.41 and 0.34 for LNCaP, LNN-1 and untreated LNX-1, while this range was reduced to 0.25 after LNX-1 TGIFLX induction by doxycyclin (P<0.005). Data has shown lower metabolic activity in LNX-1 treated with doxycyclin compared with untreated cells LNN-1.

Bottom Line: TGIFLX gene expression was confirmed by RT-PCR.Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P<0.01).The TGIFLX expression was confirmed by RT-PCR and fluorescent microscopy.

View Article: PubMed Central - PubMed

Affiliation: 1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.

ABSTRACT

Background: TGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains unknown. The purpose of this study was to evaluate the consequences of TGIFLX expression on prostate cancer cell lines (LNCaP).

Method: Inducible Tet-On gene expression system was used with a regulatory capability by doxycycline induction. In this system, stable LNCaP cells with TGIFLX tet-on plasmid were able to induce TGIFLX expression by doxycycline treatment. TGIFLX gene expression was confirmed by RT-PCR.

Results: Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P<0.01). Also, by using PEGFPN1 plasmid protein in this study localization was shown in nucleus. The gene was cloned in the plasmid and transfected to LNcap cells with plasmid PEGFPN1 TGIFLX and the plasmid was PEGFPN1. The TGIFLX expression was confirmed by RT-PCR and fluorescent microscopy.

Conclusion: TGIFLX expression demonstrated a tumor suppressor characterization in a prostatic cancer cell line with low grade of tumorigenicity (LNCaP). More cell lines with different level of tumorogenicity need to be investigated for further clarification of the TGIFLX gene function.

No MeSH data available.


Related in: MedlinePlus