Limits...
A Methionine Deficient Diet Enhances Adipose Tissue Lipid Metabolism and Alters Anti-Oxidant Pathways in Young Growing Pigs.

Castellano R, Perruchot MH, Conde-Aguilera JA, van Milgen J, Collin A, Tesseraud S, Mercier Y, Gondret F - PLoS ONE (2015)

Bottom Line: Methionine is a rate-limiting amino-acid for protein synthesis but non-proteinogenic roles on lipid metabolism and oxidative stress have been demonstrated.These results show that a dietary methionine deficiency resulted in increased levels of lipogenesis and lipolytic indicators in porcine adipose tissues.These findings illustrate that a rapidly growing animal differently adapts tissue metabolisms when facing an insufficient methionine supply.

View Article: PubMed Central - PubMed

Affiliation: UMR1348 Physiologie, Environnement, et Génétique pour l'Animal et les Systèmes d'Elevage (UMR PEGASE), INRA, Saint-Gilles, France; UMR1348 Physiologie, Environnement, et Génétique pour l'Animal et les Systèmes d'Elevage (UMR PEGASE), Agrocampus-Ouest, Rennes, France.

ABSTRACT
Methionine is a rate-limiting amino-acid for protein synthesis but non-proteinogenic roles on lipid metabolism and oxidative stress have been demonstrated. Contrary to rodents where a dietary methionine deficiency led to a lower adiposity, an increased lipid accretion rate has been reported in growing pigs fed a methionine deficient diet. This study aimed to clarify the effects of a dietary methionine deficiency on different aspects of tissue lipid metabolism and anti-oxidant pathways in young pigs. Post-weaned pigs (9.8 kg initial body weight) were restrictively-fed diets providing either an adequate (CTRL) or a deficient methionine supply (MD) during 10 days (n=6 per group). At the end of the feeding trial, pigs fed the MD diet had higher lipid content in subcutaneous adipose tissue. Expression levels of genes involved in glucose uptake, lipogenesis but also lipolysis, and activities of NADPH enzyme suppliers were generally higher in subcutaneous and perirenal adipose tissues of MD pigs, suggesting an increased lipid turnover in those pigs. Activities of the anti-oxidant enzymes superoxide dismutase, catalase and glutathione reductase were increased in adipose tissues and muscle of MD pigs. Expression level and activity of the glutathione peroxidase were also higher in liver of MD pigs, but hepatic contents in the reduced and oxidized forms of glutathione and glutathione reductase activity were lower compared with control pigs. In plasma, superoxide dismutase activity was higher but total anti-oxidant power was lower in MD pigs. These results show that a dietary methionine deficiency resulted in increased levels of lipogenesis and lipolytic indicators in porcine adipose tissues. Decreased glutathione content in the liver and coordinated increase of enzymatic antioxidant activities in adipose tissues altered the cellular redox status of young pigs fed a methionine-deficient diet. These findings illustrate that a rapidly growing animal differently adapts tissue metabolisms when facing an insufficient methionine supply.

No MeSH data available.


Related in: MedlinePlus

Expression levels of genes involved in lipid metabolism in porcine adipose tissues. Pigs (42 d of age) were fed either a methionine-deficient (MD) or a CTRL diet providing an adequate level of methionine for 10 days (n = 6 per diet). Values are least square means together with residual standard deviation (RSD). Differences between groups were significant at ***P<0.001, **P≤0.01, and *P≤0.05; t denotes a tendency between treated groups (0.05<P<0.10). Abbreviations used: PRAT, perirenal fat; SCAT, dorsal subcutaneous adipose tissue. The mRNA levels (arbitrary units) of the target genes were measured by quantitative real time polymerase chain reaction (RT-qPCR).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4498751&req=5

pone.0130514.g001: Expression levels of genes involved in lipid metabolism in porcine adipose tissues. Pigs (42 d of age) were fed either a methionine-deficient (MD) or a CTRL diet providing an adequate level of methionine for 10 days (n = 6 per diet). Values are least square means together with residual standard deviation (RSD). Differences between groups were significant at ***P<0.001, **P≤0.01, and *P≤0.05; t denotes a tendency between treated groups (0.05<P<0.10). Abbreviations used: PRAT, perirenal fat; SCAT, dorsal subcutaneous adipose tissue. The mRNA levels (arbitrary units) of the target genes were measured by quantitative real time polymerase chain reaction (RT-qPCR).

Mentions: In SCAT as well as in PRAT, the MD pigs exhibited an up-regulation of different genes involved in glucose uptake (GLUT4; +55% on average; P<0.05), lipogenesis (ME1 and FASN: +121% and +30% on average, respectively; P<0.05) and fatty acid transport (CD36; +25% on average; P<0.05), but LPL expression did not significantly differ between the two groups (Fig 1). The expression levels of two genes participating to lipolysis (HSL and ATGL) were also higher (P<0.05) in the adipose tissues of MD pigs. Expression level of the fatty acid binding protein (FABP4) did not differ between treatments in SCAT, but it was increased in PRAT of MD pigs compared with CTRL pigs (+32%; P<0.01). In muscle, ME1 coding for malic enzyme was more expressed (+53%, P<0.05) in MD pigs than in CTRL pigs; however, mRNA levels of other lipid-related genes did not differ between groups (data not shown).


A Methionine Deficient Diet Enhances Adipose Tissue Lipid Metabolism and Alters Anti-Oxidant Pathways in Young Growing Pigs.

Castellano R, Perruchot MH, Conde-Aguilera JA, van Milgen J, Collin A, Tesseraud S, Mercier Y, Gondret F - PLoS ONE (2015)

Expression levels of genes involved in lipid metabolism in porcine adipose tissues. Pigs (42 d of age) were fed either a methionine-deficient (MD) or a CTRL diet providing an adequate level of methionine for 10 days (n = 6 per diet). Values are least square means together with residual standard deviation (RSD). Differences between groups were significant at ***P<0.001, **P≤0.01, and *P≤0.05; t denotes a tendency between treated groups (0.05<P<0.10). Abbreviations used: PRAT, perirenal fat; SCAT, dorsal subcutaneous adipose tissue. The mRNA levels (arbitrary units) of the target genes were measured by quantitative real time polymerase chain reaction (RT-qPCR).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4498751&req=5

pone.0130514.g001: Expression levels of genes involved in lipid metabolism in porcine adipose tissues. Pigs (42 d of age) were fed either a methionine-deficient (MD) or a CTRL diet providing an adequate level of methionine for 10 days (n = 6 per diet). Values are least square means together with residual standard deviation (RSD). Differences between groups were significant at ***P<0.001, **P≤0.01, and *P≤0.05; t denotes a tendency between treated groups (0.05<P<0.10). Abbreviations used: PRAT, perirenal fat; SCAT, dorsal subcutaneous adipose tissue. The mRNA levels (arbitrary units) of the target genes were measured by quantitative real time polymerase chain reaction (RT-qPCR).
Mentions: In SCAT as well as in PRAT, the MD pigs exhibited an up-regulation of different genes involved in glucose uptake (GLUT4; +55% on average; P<0.05), lipogenesis (ME1 and FASN: +121% and +30% on average, respectively; P<0.05) and fatty acid transport (CD36; +25% on average; P<0.05), but LPL expression did not significantly differ between the two groups (Fig 1). The expression levels of two genes participating to lipolysis (HSL and ATGL) were also higher (P<0.05) in the adipose tissues of MD pigs. Expression level of the fatty acid binding protein (FABP4) did not differ between treatments in SCAT, but it was increased in PRAT of MD pigs compared with CTRL pigs (+32%; P<0.01). In muscle, ME1 coding for malic enzyme was more expressed (+53%, P<0.05) in MD pigs than in CTRL pigs; however, mRNA levels of other lipid-related genes did not differ between groups (data not shown).

Bottom Line: Methionine is a rate-limiting amino-acid for protein synthesis but non-proteinogenic roles on lipid metabolism and oxidative stress have been demonstrated.These results show that a dietary methionine deficiency resulted in increased levels of lipogenesis and lipolytic indicators in porcine adipose tissues.These findings illustrate that a rapidly growing animal differently adapts tissue metabolisms when facing an insufficient methionine supply.

View Article: PubMed Central - PubMed

Affiliation: UMR1348 Physiologie, Environnement, et Génétique pour l'Animal et les Systèmes d'Elevage (UMR PEGASE), INRA, Saint-Gilles, France; UMR1348 Physiologie, Environnement, et Génétique pour l'Animal et les Systèmes d'Elevage (UMR PEGASE), Agrocampus-Ouest, Rennes, France.

ABSTRACT
Methionine is a rate-limiting amino-acid for protein synthesis but non-proteinogenic roles on lipid metabolism and oxidative stress have been demonstrated. Contrary to rodents where a dietary methionine deficiency led to a lower adiposity, an increased lipid accretion rate has been reported in growing pigs fed a methionine deficient diet. This study aimed to clarify the effects of a dietary methionine deficiency on different aspects of tissue lipid metabolism and anti-oxidant pathways in young pigs. Post-weaned pigs (9.8 kg initial body weight) were restrictively-fed diets providing either an adequate (CTRL) or a deficient methionine supply (MD) during 10 days (n=6 per group). At the end of the feeding trial, pigs fed the MD diet had higher lipid content in subcutaneous adipose tissue. Expression levels of genes involved in glucose uptake, lipogenesis but also lipolysis, and activities of NADPH enzyme suppliers were generally higher in subcutaneous and perirenal adipose tissues of MD pigs, suggesting an increased lipid turnover in those pigs. Activities of the anti-oxidant enzymes superoxide dismutase, catalase and glutathione reductase were increased in adipose tissues and muscle of MD pigs. Expression level and activity of the glutathione peroxidase were also higher in liver of MD pigs, but hepatic contents in the reduced and oxidized forms of glutathione and glutathione reductase activity were lower compared with control pigs. In plasma, superoxide dismutase activity was higher but total anti-oxidant power was lower in MD pigs. These results show that a dietary methionine deficiency resulted in increased levels of lipogenesis and lipolytic indicators in porcine adipose tissues. Decreased glutathione content in the liver and coordinated increase of enzymatic antioxidant activities in adipose tissues altered the cellular redox status of young pigs fed a methionine-deficient diet. These findings illustrate that a rapidly growing animal differently adapts tissue metabolisms when facing an insufficient methionine supply.

No MeSH data available.


Related in: MedlinePlus