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N-Propionylmannosamine stimulates axonal elongation in a murine model of sciatic nerve injury.

Witzel C, Reutter W, Stark GB, Koulaxouzidis G - Neural Regen Res (2015)

Bottom Line: ManNProp significantly increased the mean distance of axonal regeneration (2.49 mm vs. 1.53 mm; P < 0.005) and the number of arborizing axons (21% vs. 16%; P = 0.008) 5 days after sciatic nerve grafting.ManNProp did not affect the number of regenerating axons or the number of branches per arborizing axon.The biochemical glycoengineering of the N-acyl side chain of sialic acid might be a promising approach for improving peripheral nerve regeneration.

View Article: PubMed Central - PubMed

Affiliation: Plastic and Reconstructive Surgery, Interdisciplinary Breast Center, Charité - Universitätsmedizin Berlin, Germany.

ABSTRACT
Increasing evidence indicates that sialic acid plays an important role during nerve regeneration. Sialic acids can be modified in vitro as well as in vivo using metabolic oligosaccharide engineering of the N-acyl side chain. N-Propionylmannosamine (ManNProp) increases neurite outgrowth and accelerates the reestablishment of functional synapses in vitro. We investigated the influence of systemic ManNProp application using a specific in vivo mouse model. Using mice expressing axonal fluorescent proteins, we quantified the extension of regenerating axons, the number of regenerating axons, the number of arborising axons and the number of branches per axon 5 days after injury. Sciatic nerves from non-expressing mice were grafted into those expressing yellow fluorescent protein. We began a twice-daily intraperitoneal application of either peracetylated ManNProp (200 mg/kg) or saline solution 5 days before injury, and continued it until nerve harvest (5 days after transection). ManNProp significantly increased the mean distance of axonal regeneration (2.49 mm vs. 1.53 mm; P < 0.005) and the number of arborizing axons (21% vs. 16%; P = 0.008) 5 days after sciatic nerve grafting. ManNProp did not affect the number of regenerating axons or the number of branches per arborizing axon. The biochemical glycoengineering of the N-acyl side chain of sialic acid might be a promising approach for improving peripheral nerve regeneration.

No MeSH data available.


Related in: MedlinePlus

Treatment with N-propionylmannosamine (ManNProp) did not affect the number of branches per regenerating axon.The mean value of branches per regenerating axon in the ManNProp group was 0.80 ± 0.07 (saline group: 0.82 ± 0.18; P > 0.05; mean ± SD; Student's t-test; n = 6 for each group). The number was calculated as the ratio of the number of arborizing axons to the total number of regenerating axons.
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Figure 6: Treatment with N-propionylmannosamine (ManNProp) did not affect the number of branches per regenerating axon.The mean value of branches per regenerating axon in the ManNProp group was 0.80 ± 0.07 (saline group: 0.82 ± 0.18; P > 0.05; mean ± SD; Student's t-test; n = 6 for each group). The number was calculated as the ratio of the number of arborizing axons to the total number of regenerating axons.

Mentions: After 5 days, the regeneration distance in the ManNProp group increased significantly compared to the saline group (P < 0.005; Figure 2). The mean regeneration distance achieved was 2.49 ± 0.05 mm for the ManNProp group and 1.53 ± 0.04 mm for the saline group. Regeneration distances of the 25th, 50th and 75th percentiles were 3, 3, and 2 mm for the ManNProp group, and 2, 1, and 1 mm for the saline group (Figure 3, Table 1). The mean percentage of arborizing regenerating axons after 5 days was higher between the ManNProp group compared with the saline group (P < 0.05; Figure 4). The difference of the number of regenerating axons between the ManNProp group and saline group was not found (P > 0.05; Figure 5). The mean number of branches per regenerating axon in the ManNProp group was similar to the saline group (P > 0.05; Figure 6). There were no differences found in the number of branches per arborizing axon between the ManNProp group and the saline group (P > 0.05; Figure 7).


N-Propionylmannosamine stimulates axonal elongation in a murine model of sciatic nerve injury.

Witzel C, Reutter W, Stark GB, Koulaxouzidis G - Neural Regen Res (2015)

Treatment with N-propionylmannosamine (ManNProp) did not affect the number of branches per regenerating axon.The mean value of branches per regenerating axon in the ManNProp group was 0.80 ± 0.07 (saline group: 0.82 ± 0.18; P > 0.05; mean ± SD; Student's t-test; n = 6 for each group). The number was calculated as the ratio of the number of arborizing axons to the total number of regenerating axons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4498362&req=5

Figure 6: Treatment with N-propionylmannosamine (ManNProp) did not affect the number of branches per regenerating axon.The mean value of branches per regenerating axon in the ManNProp group was 0.80 ± 0.07 (saline group: 0.82 ± 0.18; P > 0.05; mean ± SD; Student's t-test; n = 6 for each group). The number was calculated as the ratio of the number of arborizing axons to the total number of regenerating axons.
Mentions: After 5 days, the regeneration distance in the ManNProp group increased significantly compared to the saline group (P < 0.005; Figure 2). The mean regeneration distance achieved was 2.49 ± 0.05 mm for the ManNProp group and 1.53 ± 0.04 mm for the saline group. Regeneration distances of the 25th, 50th and 75th percentiles were 3, 3, and 2 mm for the ManNProp group, and 2, 1, and 1 mm for the saline group (Figure 3, Table 1). The mean percentage of arborizing regenerating axons after 5 days was higher between the ManNProp group compared with the saline group (P < 0.05; Figure 4). The difference of the number of regenerating axons between the ManNProp group and saline group was not found (P > 0.05; Figure 5). The mean number of branches per regenerating axon in the ManNProp group was similar to the saline group (P > 0.05; Figure 6). There were no differences found in the number of branches per arborizing axon between the ManNProp group and the saline group (P > 0.05; Figure 7).

Bottom Line: ManNProp significantly increased the mean distance of axonal regeneration (2.49 mm vs. 1.53 mm; P < 0.005) and the number of arborizing axons (21% vs. 16%; P = 0.008) 5 days after sciatic nerve grafting.ManNProp did not affect the number of regenerating axons or the number of branches per arborizing axon.The biochemical glycoengineering of the N-acyl side chain of sialic acid might be a promising approach for improving peripheral nerve regeneration.

View Article: PubMed Central - PubMed

Affiliation: Plastic and Reconstructive Surgery, Interdisciplinary Breast Center, Charité - Universitätsmedizin Berlin, Germany.

ABSTRACT
Increasing evidence indicates that sialic acid plays an important role during nerve regeneration. Sialic acids can be modified in vitro as well as in vivo using metabolic oligosaccharide engineering of the N-acyl side chain. N-Propionylmannosamine (ManNProp) increases neurite outgrowth and accelerates the reestablishment of functional synapses in vitro. We investigated the influence of systemic ManNProp application using a specific in vivo mouse model. Using mice expressing axonal fluorescent proteins, we quantified the extension of regenerating axons, the number of regenerating axons, the number of arborising axons and the number of branches per axon 5 days after injury. Sciatic nerves from non-expressing mice were grafted into those expressing yellow fluorescent protein. We began a twice-daily intraperitoneal application of either peracetylated ManNProp (200 mg/kg) or saline solution 5 days before injury, and continued it until nerve harvest (5 days after transection). ManNProp significantly increased the mean distance of axonal regeneration (2.49 mm vs. 1.53 mm; P < 0.005) and the number of arborizing axons (21% vs. 16%; P = 0.008) 5 days after sciatic nerve grafting. ManNProp did not affect the number of regenerating axons or the number of branches per arborizing axon. The biochemical glycoengineering of the N-acyl side chain of sialic acid might be a promising approach for improving peripheral nerve regeneration.

No MeSH data available.


Related in: MedlinePlus