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Introgression of the SbASR-1 gene cloned from a halophyte Salicornia brachiate enhances salinity and drought endurance in transgenic groundnut (arachis hypogaea)and acts as a transcription factor [corrected].

Tiwari V, Chaturvedi AK, Mishra A, Jha B - PLoS ONE (2015)

Bottom Line: Bipartite Nuclear Localization Signal (NLS) was found on the C-terminal end of protein and localization study confirmed that SbASR-1 is a nuclear protein.Also, lower accumulation of H2O2 and O2.- radicals was detected in transgenic lines compared to Wt plants under stress conditions.Based on results of the present study, it may be concluded that SbASR-1 enhances the salinity and drought stress tolerance in transgenic groundnut by functioning as a LEA (late embryogenesis abundant) protein and a transcription factor.

View Article: PubMed Central - PubMed

Affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.

ABSTRACT
The SbASR-1 gene, cloned from a halophyte Salicornia brachiata, encodes a plant-specific hydrophilic and stress responsive protein. The genome of S. brachiata has two paralogs of the SbASR-1 gene (2549 bp), which is comprised of a single intron of 1611 bp, the largest intron of the  abscisic acid stress ripening [ASR] gene family yet reported. In silico analysis of the 843-bp putative promoter revealed the presence of ABA, biotic stress, dehydration, phytohormone, salinity, and sugar responsive cis-regulatory motifs. The SbASR-1 protein belongs to Group 7 LEA protein family with different amino acid composition compared to their glycophytic homologs. Bipartite Nuclear Localization Signal (NLS) was found on the C-terminal end of protein and localization study confirmed that SbASR-1 is a nuclear protein. Furthermore, transgenic groundnut (Arachis hypogaea) plants over-expressing the SbASR-1 gene constitutively showed enhanced salinity and drought stress tolerance in the T1 generation. Leaves of transgenic lines exhibited higher chlorophyll and relative water contents and lower electrolyte leakage, malondialdehyde content, proline, sugars, and starch accumulation under stress treatments than wild-type (Wt) plants. Also, lower accumulation of H2O2 and O2.- radicals was detected in transgenic lines compared to Wt plants under stress conditions. Transcript expression of APX (ascorbate peroxidase) and CAT (catalase) genes were higher in Wt plants, whereas the SOD (superoxide dismutase) transcripts were higher in transgenic lines under stress. Electrophoretic mobility shift assay (EMSA) confirmed that the SbASR-1 protein binds at the consensus sequence (C/G/A)(G/T)CC(C/G)(C/G/A)(A/T). Based on results of the present study, it may be concluded that SbASR-1 enhances the salinity and drought stress tolerance in transgenic groundnut by functioning as a LEA (late embryogenesis abundant) protein and a transcription factor.

No MeSH data available.


Related in: MedlinePlus

In-vivo localisation of peroxide and superoxide free radicals.DAB (a) and NBT (b) staining of Wt plants leaves and transgenic lines leaves. Scale bar represents 5 mm of length.
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pone.0131567.g010: In-vivo localisation of peroxide and superoxide free radicals.DAB (a) and NBT (b) staining of Wt plants leaves and transgenic lines leaves. Scale bar represents 5 mm of length.

Mentions: There were no differences in peroxide localization and free radicals between leaves of Wt plants and transgenic lines under control conditions after staining with DAB and NBT. In contrast, Wt plants leaves exhibited higher levels of brown and blue-colored insoluble precipitate formation than that of transgenic lines under salinity and drought stress (Fig 10). This result demonstrates that Wt plants leaves accumulated more O2− and H2O2 than that of transgenic lines under stress, confirming that SbASR-1 helps to minimize stress-induced oxidative stress in situ.


Introgression of the SbASR-1 gene cloned from a halophyte Salicornia brachiate enhances salinity and drought endurance in transgenic groundnut (arachis hypogaea)and acts as a transcription factor [corrected].

Tiwari V, Chaturvedi AK, Mishra A, Jha B - PLoS ONE (2015)

In-vivo localisation of peroxide and superoxide free radicals.DAB (a) and NBT (b) staining of Wt plants leaves and transgenic lines leaves. Scale bar represents 5 mm of length.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4497679&req=5

pone.0131567.g010: In-vivo localisation of peroxide and superoxide free radicals.DAB (a) and NBT (b) staining of Wt plants leaves and transgenic lines leaves. Scale bar represents 5 mm of length.
Mentions: There were no differences in peroxide localization and free radicals between leaves of Wt plants and transgenic lines under control conditions after staining with DAB and NBT. In contrast, Wt plants leaves exhibited higher levels of brown and blue-colored insoluble precipitate formation than that of transgenic lines under salinity and drought stress (Fig 10). This result demonstrates that Wt plants leaves accumulated more O2− and H2O2 than that of transgenic lines under stress, confirming that SbASR-1 helps to minimize stress-induced oxidative stress in situ.

Bottom Line: Bipartite Nuclear Localization Signal (NLS) was found on the C-terminal end of protein and localization study confirmed that SbASR-1 is a nuclear protein.Also, lower accumulation of H2O2 and O2.- radicals was detected in transgenic lines compared to Wt plants under stress conditions.Based on results of the present study, it may be concluded that SbASR-1 enhances the salinity and drought stress tolerance in transgenic groundnut by functioning as a LEA (late embryogenesis abundant) protein and a transcription factor.

View Article: PubMed Central - PubMed

Affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.

ABSTRACT
The SbASR-1 gene, cloned from a halophyte Salicornia brachiata, encodes a plant-specific hydrophilic and stress responsive protein. The genome of S. brachiata has two paralogs of the SbASR-1 gene (2549 bp), which is comprised of a single intron of 1611 bp, the largest intron of the  abscisic acid stress ripening [ASR] gene family yet reported. In silico analysis of the 843-bp putative promoter revealed the presence of ABA, biotic stress, dehydration, phytohormone, salinity, and sugar responsive cis-regulatory motifs. The SbASR-1 protein belongs to Group 7 LEA protein family with different amino acid composition compared to their glycophytic homologs. Bipartite Nuclear Localization Signal (NLS) was found on the C-terminal end of protein and localization study confirmed that SbASR-1 is a nuclear protein. Furthermore, transgenic groundnut (Arachis hypogaea) plants over-expressing the SbASR-1 gene constitutively showed enhanced salinity and drought stress tolerance in the T1 generation. Leaves of transgenic lines exhibited higher chlorophyll and relative water contents and lower electrolyte leakage, malondialdehyde content, proline, sugars, and starch accumulation under stress treatments than wild-type (Wt) plants. Also, lower accumulation of H2O2 and O2.- radicals was detected in transgenic lines compared to Wt plants under stress conditions. Transcript expression of APX (ascorbate peroxidase) and CAT (catalase) genes were higher in Wt plants, whereas the SOD (superoxide dismutase) transcripts were higher in transgenic lines under stress. Electrophoretic mobility shift assay (EMSA) confirmed that the SbASR-1 protein binds at the consensus sequence (C/G/A)(G/T)CC(C/G)(C/G/A)(A/T). Based on results of the present study, it may be concluded that SbASR-1 enhances the salinity and drought stress tolerance in transgenic groundnut by functioning as a LEA (late embryogenesis abundant) protein and a transcription factor.

No MeSH data available.


Related in: MedlinePlus