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Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model.

Ozeki N, Muneta T, Matsuta S, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Saito T, Sekiya I - Stem Cells (2015)

Bottom Line: The meniscus and surrounding cartilage were evaluated at 2, 4, and 8 weeks (n = 5).Flow cytometric analysis showed that transplanted synovial MSCs retained their MSC properties at 7 days and host synovial tissue also contained cells with MSC characteristics.Synovial MSCs promoted meniscus regeneration augmented by autologous Achilles tendon grafts and prevented cartilage degeneration in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Joint Surgery and Sports medicine.

No MeSH data available.


Related in: MedlinePlus

Analyses of mesenchymal stem cell (MSC) properties after engraftment and contribution of host knee tissue. (A): Schematic representation of the flow cytomeric assay of green fluorescence protein (GFP+) MSCs around the regenerating meniscus (n = 2). (B): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. (C): Expression of the indicated markers in CD90 and GFP double positive MSCs. Shown is the percentage of cells that express the antigen (red line) versus a matched isotype control (blue line). (D): Schematic representation of the analysis of the contribution of host knee tissue (n = 2). (E): Macroscopic analysis for GFP detection of grafted WT tendon into GFP+ rat knee. White dotted lines indicate the native meniscus. (F): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. Abbreviations: GFP, green fluorescence protein; MSC, mesenchymal stem cell; WT, wild type.
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fig05: Analyses of mesenchymal stem cell (MSC) properties after engraftment and contribution of host knee tissue. (A): Schematic representation of the flow cytomeric assay of green fluorescence protein (GFP+) MSCs around the regenerating meniscus (n = 2). (B): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. (C): Expression of the indicated markers in CD90 and GFP double positive MSCs. Shown is the percentage of cells that express the antigen (red line) versus a matched isotype control (blue line). (D): Schematic representation of the analysis of the contribution of host knee tissue (n = 2). (E): Macroscopic analysis for GFP detection of grafted WT tendon into GFP+ rat knee. White dotted lines indicate the native meniscus. (F): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. Abbreviations: GFP, green fluorescence protein; MSC, mesenchymal stem cell; WT, wild type.

Mentions: Next, GFP+ MSCs were transplanted, and GFP+ MSCs that would contribute to meniscus regeneration were analyzed using flow cytometry (Fig. 5A). At Day 1, GFP+ cells comprised 1.1% of the regenerating meniscus, and nearly all of these GFP+ cells were positive for CD90 (Fig. 5B). The cells were also positive for CD29, while negative for CD31 and CD45 (Fig. 5C) and this expression patterns was the same at Day 7 (Fig. 5B, 5C).


Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model.

Ozeki N, Muneta T, Matsuta S, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Saito T, Sekiya I - Stem Cells (2015)

Analyses of mesenchymal stem cell (MSC) properties after engraftment and contribution of host knee tissue. (A): Schematic representation of the flow cytomeric assay of green fluorescence protein (GFP+) MSCs around the regenerating meniscus (n = 2). (B): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. (C): Expression of the indicated markers in CD90 and GFP double positive MSCs. Shown is the percentage of cells that express the antigen (red line) versus a matched isotype control (blue line). (D): Schematic representation of the analysis of the contribution of host knee tissue (n = 2). (E): Macroscopic analysis for GFP detection of grafted WT tendon into GFP+ rat knee. White dotted lines indicate the native meniscus. (F): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. Abbreviations: GFP, green fluorescence protein; MSC, mesenchymal stem cell; WT, wild type.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4497612&req=5

fig05: Analyses of mesenchymal stem cell (MSC) properties after engraftment and contribution of host knee tissue. (A): Schematic representation of the flow cytomeric assay of green fluorescence protein (GFP+) MSCs around the regenerating meniscus (n = 2). (B): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. (C): Expression of the indicated markers in CD90 and GFP double positive MSCs. Shown is the percentage of cells that express the antigen (red line) versus a matched isotype control (blue line). (D): Schematic representation of the analysis of the contribution of host knee tissue (n = 2). (E): Macroscopic analysis for GFP detection of grafted WT tendon into GFP+ rat knee. White dotted lines indicate the native meniscus. (F): Representative flow cytometric profiles of regenerating meniscus for GFP+ and CD90+ cells. Abbreviations: GFP, green fluorescence protein; MSC, mesenchymal stem cell; WT, wild type.
Mentions: Next, GFP+ MSCs were transplanted, and GFP+ MSCs that would contribute to meniscus regeneration were analyzed using flow cytometry (Fig. 5A). At Day 1, GFP+ cells comprised 1.1% of the regenerating meniscus, and nearly all of these GFP+ cells were positive for CD90 (Fig. 5B). The cells were also positive for CD29, while negative for CD31 and CD45 (Fig. 5C) and this expression patterns was the same at Day 7 (Fig. 5B, 5C).

Bottom Line: The meniscus and surrounding cartilage were evaluated at 2, 4, and 8 weeks (n = 5).Flow cytometric analysis showed that transplanted synovial MSCs retained their MSC properties at 7 days and host synovial tissue also contained cells with MSC characteristics.Synovial MSCs promoted meniscus regeneration augmented by autologous Achilles tendon grafts and prevented cartilage degeneration in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Joint Surgery and Sports medicine.

No MeSH data available.


Related in: MedlinePlus