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Propionic acid and butyric acid inhibit lipolysis and de novo lipogenesis and increase insulin-stimulated glucose uptake in primary rat adipocytes.

Heimann E, Nyman M, Degerman E - Adipocyte (2014)

Bottom Line: In addition, we show that propionic acid and butyric acid inhibit basal and insulin-stimulated de novo lipogenesis, which is associated with increased phosphorylation and thus inhibition of acetyl CoA carboxylase, a rate-limiting enzyme in fatty acid synthesis.To conclude, our study shows that SCFAs have effects on fat storage and mobilization as well as glucose uptake in rat primary adipocytes.Thus, the SCFAs might contribute to healthier adipocytes and subsequently also to improved energy metabolism with for example less circulating free fatty acids, which is beneficial in the context of obesity and type 2 diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medical Science; Lund University ; Lund, Sweden.

ABSTRACT
Fermentation of dietary fibers by colonic microbiota generates short-chain fatty acids (SCFAs), e.g., propionic acid and butyric acid, which have been described to have "anti-obesity properties" by ameliorating fasting glycaemia, body weight and insulin tolerance in animal models. In the present study, we therefore investigate if propionic acid and butyric acid have effects on lipolysis, de novo lipogenesis and glucose uptake in primary rat adipocytes. We show that both propionic acid and butyric acid inhibit isoproterenol- and adenosine deaminase-stimulated lipolysis as well as isoproterenol-stimulated lipolysis in the presence of a phosphodiesterase (PDE3) inhibitor. In addition, we show that propionic acid and butyric acid inhibit basal and insulin-stimulated de novo lipogenesis, which is associated with increased phosphorylation and thus inhibition of acetyl CoA carboxylase, a rate-limiting enzyme in fatty acid synthesis. Furthermore, we show that propionic acid and butyric acid increase insulin-stimulated glucose uptake. To conclude, our study shows that SCFAs have effects on fat storage and mobilization as well as glucose uptake in rat primary adipocytes. Thus, the SCFAs might contribute to healthier adipocytes and subsequently also to improved energy metabolism with for example less circulating free fatty acids, which is beneficial in the context of obesity and type 2 diabetes.

No MeSH data available.


Related in: MedlinePlus

Short-chain fatty acids potentiate insulin-stimulated glucose uptake in primary adipocytes. Glucose uptake was measured after 30 min of stimulation with or without 1 nM insulin (INS) in the presence or absence of 1, 3 and 10 mM propionic acid (PA) (A and B) or butyric acid (BA) (C and D). In A and C, the values are related to BASAL CTRL, the condition without insulin and SCFA. In B and D, the values are based upon the ratio between BASAL and INS-stimulated conditions (shown in A and C). Mean ± SD (n = 4–5) were used and significance levels were accepted when *P < 0.05, **P < 0.01 and ***P < 0.001.
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f0004: Short-chain fatty acids potentiate insulin-stimulated glucose uptake in primary adipocytes. Glucose uptake was measured after 30 min of stimulation with or without 1 nM insulin (INS) in the presence or absence of 1, 3 and 10 mM propionic acid (PA) (A and B) or butyric acid (BA) (C and D). In A and C, the values are related to BASAL CTRL, the condition without insulin and SCFA. In B and D, the values are based upon the ratio between BASAL and INS-stimulated conditions (shown in A and C). Mean ± SD (n = 4–5) were used and significance levels were accepted when *P < 0.05, **P < 0.01 and ***P < 0.001.

Mentions: The effect of propionic acid and butyric acid on glucose uptake was studied in primary rat adipocytes. As shown in Figure 4A and C, basal glucose uptake was significantly decreased by both propionic acid and butyric acid. However, insulin-stimulated glucose uptake was significantly increased in the presence of propionic acid (Fig. 4A and B) due to decreased and increased glucose uptake in the absence or presence of insulin, respectively. Butyric acid did also have an effect on insulin-stimulated glucose uptake (Fig. 4C), however, the effect of butyric acid, as shown in Figure 4D, is solely due to a decreased basal glucose uptake in the presence of butyric acid (10 mM).Figure 4.


Propionic acid and butyric acid inhibit lipolysis and de novo lipogenesis and increase insulin-stimulated glucose uptake in primary rat adipocytes.

Heimann E, Nyman M, Degerman E - Adipocyte (2014)

Short-chain fatty acids potentiate insulin-stimulated glucose uptake in primary adipocytes. Glucose uptake was measured after 30 min of stimulation with or without 1 nM insulin (INS) in the presence or absence of 1, 3 and 10 mM propionic acid (PA) (A and B) or butyric acid (BA) (C and D). In A and C, the values are related to BASAL CTRL, the condition without insulin and SCFA. In B and D, the values are based upon the ratio between BASAL and INS-stimulated conditions (shown in A and C). Mean ± SD (n = 4–5) were used and significance levels were accepted when *P < 0.05, **P < 0.01 and ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496978&req=5

f0004: Short-chain fatty acids potentiate insulin-stimulated glucose uptake in primary adipocytes. Glucose uptake was measured after 30 min of stimulation with or without 1 nM insulin (INS) in the presence or absence of 1, 3 and 10 mM propionic acid (PA) (A and B) or butyric acid (BA) (C and D). In A and C, the values are related to BASAL CTRL, the condition without insulin and SCFA. In B and D, the values are based upon the ratio between BASAL and INS-stimulated conditions (shown in A and C). Mean ± SD (n = 4–5) were used and significance levels were accepted when *P < 0.05, **P < 0.01 and ***P < 0.001.
Mentions: The effect of propionic acid and butyric acid on glucose uptake was studied in primary rat adipocytes. As shown in Figure 4A and C, basal glucose uptake was significantly decreased by both propionic acid and butyric acid. However, insulin-stimulated glucose uptake was significantly increased in the presence of propionic acid (Fig. 4A and B) due to decreased and increased glucose uptake in the absence or presence of insulin, respectively. Butyric acid did also have an effect on insulin-stimulated glucose uptake (Fig. 4C), however, the effect of butyric acid, as shown in Figure 4D, is solely due to a decreased basal glucose uptake in the presence of butyric acid (10 mM).Figure 4.

Bottom Line: In addition, we show that propionic acid and butyric acid inhibit basal and insulin-stimulated de novo lipogenesis, which is associated with increased phosphorylation and thus inhibition of acetyl CoA carboxylase, a rate-limiting enzyme in fatty acid synthesis.To conclude, our study shows that SCFAs have effects on fat storage and mobilization as well as glucose uptake in rat primary adipocytes.Thus, the SCFAs might contribute to healthier adipocytes and subsequently also to improved energy metabolism with for example less circulating free fatty acids, which is beneficial in the context of obesity and type 2 diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medical Science; Lund University ; Lund, Sweden.

ABSTRACT
Fermentation of dietary fibers by colonic microbiota generates short-chain fatty acids (SCFAs), e.g., propionic acid and butyric acid, which have been described to have "anti-obesity properties" by ameliorating fasting glycaemia, body weight and insulin tolerance in animal models. In the present study, we therefore investigate if propionic acid and butyric acid have effects on lipolysis, de novo lipogenesis and glucose uptake in primary rat adipocytes. We show that both propionic acid and butyric acid inhibit isoproterenol- and adenosine deaminase-stimulated lipolysis as well as isoproterenol-stimulated lipolysis in the presence of a phosphodiesterase (PDE3) inhibitor. In addition, we show that propionic acid and butyric acid inhibit basal and insulin-stimulated de novo lipogenesis, which is associated with increased phosphorylation and thus inhibition of acetyl CoA carboxylase, a rate-limiting enzyme in fatty acid synthesis. Furthermore, we show that propionic acid and butyric acid increase insulin-stimulated glucose uptake. To conclude, our study shows that SCFAs have effects on fat storage and mobilization as well as glucose uptake in rat primary adipocytes. Thus, the SCFAs might contribute to healthier adipocytes and subsequently also to improved energy metabolism with for example less circulating free fatty acids, which is beneficial in the context of obesity and type 2 diabetes.

No MeSH data available.


Related in: MedlinePlus