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The Effects of an Extract of Atractylodes Japonica Rhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs.

Park JJ, Chon NR, Lee YJ, Park H - J Neurogastroenterol Motil (2015)

Bottom Line: The extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states.In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts.In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were sig-nificantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: There are limited therapeutic options available for irritable bowel syndrome with diarrhea (IBS-D). We tested the effects of Atractylodes japonica rhizome, a perennial plant native to North Asia, on both upper and lower gastrointestinal (GI) motility in guinea pigs.

Methods: The extract of A. japonica rhizome was administered orally at different doses to test its effects on upper GI motility as determined from charcoal transit in native guinea pigs and in guinea pigs pretreated with thyrotropin-releasing hormone or mustard oil. Regarding its effect on lower GI motility, the removed guinea pig colon was suspended in a chamber containing Krebs-Henseleit solution and the transit time of artificial feces was measured with various dilutions of the extract. As for in vivo assay, weight and number of fecal pellets expelled were determined under the same drug preparation used in upper GI motility experiment.

Results: The extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states. However, the extract increased colonic transit time in the in vitro model. In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts. In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were sig-nificantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract.

Conclusions: Our findings suggest that the extract of A. japonica rhizome can be a potential agent for IBS-D.

No MeSH data available.


Related in: MedlinePlus

Effect of SKI3246 on colonic transit time in isolated colonic segments from guinea pigs (n = 5). The colonic transit time was significantly increased at higher concentrations of SKI3246 compared with the control group (75 μg/mL and 100 μg/mL, both groups P = 0.043) and the effect was dose-dependent (Wilcoxon signed rank test).
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f1-jnm-21-352: Effect of SKI3246 on colonic transit time in isolated colonic segments from guinea pigs (n = 5). The colonic transit time was significantly increased at higher concentrations of SKI3246 compared with the control group (75 μg/mL and 100 μg/mL, both groups P = 0.043) and the effect was dose-dependent (Wilcoxon signed rank test).

Mentions: In comparison to control treatment, SKI3246 at the lowest concentration used (50 μg/mL), had no effect on colonic transit time. However, the mean colonic transit time was significantly increased at higher SKI3246 concentrations (2.8 times at 75 μg/mL and 11.4 times at 100 μg/mL, both groups P = 0.043), and the effect showed a dose-dependent tendency (Fig. 1).


The Effects of an Extract of Atractylodes Japonica Rhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs.

Park JJ, Chon NR, Lee YJ, Park H - J Neurogastroenterol Motil (2015)

Effect of SKI3246 on colonic transit time in isolated colonic segments from guinea pigs (n = 5). The colonic transit time was significantly increased at higher concentrations of SKI3246 compared with the control group (75 μg/mL and 100 μg/mL, both groups P = 0.043) and the effect was dose-dependent (Wilcoxon signed rank test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496911&req=5

f1-jnm-21-352: Effect of SKI3246 on colonic transit time in isolated colonic segments from guinea pigs (n = 5). The colonic transit time was significantly increased at higher concentrations of SKI3246 compared with the control group (75 μg/mL and 100 μg/mL, both groups P = 0.043) and the effect was dose-dependent (Wilcoxon signed rank test).
Mentions: In comparison to control treatment, SKI3246 at the lowest concentration used (50 μg/mL), had no effect on colonic transit time. However, the mean colonic transit time was significantly increased at higher SKI3246 concentrations (2.8 times at 75 μg/mL and 11.4 times at 100 μg/mL, both groups P = 0.043), and the effect showed a dose-dependent tendency (Fig. 1).

Bottom Line: The extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states.In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts.In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were sig-nificantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: There are limited therapeutic options available for irritable bowel syndrome with diarrhea (IBS-D). We tested the effects of Atractylodes japonica rhizome, a perennial plant native to North Asia, on both upper and lower gastrointestinal (GI) motility in guinea pigs.

Methods: The extract of A. japonica rhizome was administered orally at different doses to test its effects on upper GI motility as determined from charcoal transit in native guinea pigs and in guinea pigs pretreated with thyrotropin-releasing hormone or mustard oil. Regarding its effect on lower GI motility, the removed guinea pig colon was suspended in a chamber containing Krebs-Henseleit solution and the transit time of artificial feces was measured with various dilutions of the extract. As for in vivo assay, weight and number of fecal pellets expelled were determined under the same drug preparation used in upper GI motility experiment.

Results: The extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states. However, the extract increased colonic transit time in the in vitro model. In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts. In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were sig-nificantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract.

Conclusions: Our findings suggest that the extract of A. japonica rhizome can be a potential agent for IBS-D.

No MeSH data available.


Related in: MedlinePlus