Limits...
Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs.

Muñoz-González S, Perez-Simó M, Muñoz M, Bohorquez JA, Rosell R, Summerfield A, Domingo M, Ruggli N, Ganges L - Vet. Res. (2015)

Bottom Line: The humoral and interferon gamma responses as well as the CSFV RNA loads were monitored for 21 days post-vaccination.Furthermore, no E2-specific antibody response or neutralising antibody titres were shown in CSFV persistently infected vaccinated animals.Likewise, no of IFN-gamma producing cell response against CSFV or PHA was observed.

View Article: PubMed Central - PubMed

Affiliation: Centre de Recerca en Sanitat Animal (CReSA)-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. sara.munoz@cresa.uab.es.

ABSTRACT
Classical swine fever (CSF) causes major losses in pig farming, with various degrees of disease severity. Efficient live attenuated vaccines against classical swine fever virus (CSFV) are used routinely in endemic countries. However, despite intensive vaccination programs in these areas for more than 20 years, CSF has not been eradicated. Molecular epidemiology studies in these regions suggests that the virus circulating in the field has evolved under the positive selection pressure exerted by the immune response to the vaccine, leading to new attenuated viral variants. Recent work by our group demonstrated that a high proportion of persistently infected piglets can be generated by early postnatal infection with low and moderately virulent CSFV strains. Here, we studied the immune response to a hog cholera lapinised virus vaccine (HCLV), C-strain, in six-week-old persistently infected pigs following post-natal infection. CSFV-negative pigs were vaccinated as controls. The humoral and interferon gamma responses as well as the CSFV RNA loads were monitored for 21 days post-vaccination. No vaccine viral RNA was detected in the serum samples and tonsils from CSFV postnatally persistently infected pigs for 21 days post-vaccination. Furthermore, no E2-specific antibody response or neutralising antibody titres were shown in CSFV persistently infected vaccinated animals. Likewise, no of IFN-gamma producing cell response against CSFV or PHA was observed. To our knowledge, this is the first report demonstrating the absence of a response to vaccination in CSFV persistently infected pigs.

No MeSH data available.


Related in: MedlinePlus

E2-specific antibody detection and neutralising activity during 21 days post-vaccination. CSFV persistently infected-vaccinated pigs (Group 1: numbers 1 to 4) and Pestivirus-Free vaccinated pigs (Group 2: numbers 5 to 8). A) Antibody response against the E2 glycoprotein detected by ELISA (in blocking %) at 0, 4, 8, 13, 15 and 21 days post-vaccination. Values greater than 40% were considered positive (indicated by a black dotted bar). B) Neutralising antibodies titres at 15 and 21 dpv. * This animal was euthanized at 16 dpv.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4496848&req=5

Fig4: E2-specific antibody detection and neutralising activity during 21 days post-vaccination. CSFV persistently infected-vaccinated pigs (Group 1: numbers 1 to 4) and Pestivirus-Free vaccinated pigs (Group 2: numbers 5 to 8). A) Antibody response against the E2 glycoprotein detected by ELISA (in blocking %) at 0, 4, 8, 13, 15 and 21 days post-vaccination. Values greater than 40% were considered positive (indicated by a black dotted bar). B) Neutralising antibodies titres at 15 and 21 dpv. * This animal was euthanized at 16 dpv.

Mentions: To evaluate the induction of CSFV-specific antibodies, serum samples were analysed at different times after vaccination. All vaccinated pigs from Group 2 showed E2-specific antibodies response detected by ELISA from 15 to 21 dpv (Figure 4A). Likewise, neutralising antibody titres were detected at 15 and 21 dpv (Figure 4B). In contrast, an absence of antibody response, in terms of E2-specific antibodies and neutralising titres, was found in all CSFV persistently infected-vaccinated pigs (Group 1) during the entire experiment (Figures 4A and B).Figure 4


Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs.

Muñoz-González S, Perez-Simó M, Muñoz M, Bohorquez JA, Rosell R, Summerfield A, Domingo M, Ruggli N, Ganges L - Vet. Res. (2015)

E2-specific antibody detection and neutralising activity during 21 days post-vaccination. CSFV persistently infected-vaccinated pigs (Group 1: numbers 1 to 4) and Pestivirus-Free vaccinated pigs (Group 2: numbers 5 to 8). A) Antibody response against the E2 glycoprotein detected by ELISA (in blocking %) at 0, 4, 8, 13, 15 and 21 days post-vaccination. Values greater than 40% were considered positive (indicated by a black dotted bar). B) Neutralising antibodies titres at 15 and 21 dpv. * This animal was euthanized at 16 dpv.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4496848&req=5

Fig4: E2-specific antibody detection and neutralising activity during 21 days post-vaccination. CSFV persistently infected-vaccinated pigs (Group 1: numbers 1 to 4) and Pestivirus-Free vaccinated pigs (Group 2: numbers 5 to 8). A) Antibody response against the E2 glycoprotein detected by ELISA (in blocking %) at 0, 4, 8, 13, 15 and 21 days post-vaccination. Values greater than 40% were considered positive (indicated by a black dotted bar). B) Neutralising antibodies titres at 15 and 21 dpv. * This animal was euthanized at 16 dpv.
Mentions: To evaluate the induction of CSFV-specific antibodies, serum samples were analysed at different times after vaccination. All vaccinated pigs from Group 2 showed E2-specific antibodies response detected by ELISA from 15 to 21 dpv (Figure 4A). Likewise, neutralising antibody titres were detected at 15 and 21 dpv (Figure 4B). In contrast, an absence of antibody response, in terms of E2-specific antibodies and neutralising titres, was found in all CSFV persistently infected-vaccinated pigs (Group 1) during the entire experiment (Figures 4A and B).Figure 4

Bottom Line: The humoral and interferon gamma responses as well as the CSFV RNA loads were monitored for 21 days post-vaccination.Furthermore, no E2-specific antibody response or neutralising antibody titres were shown in CSFV persistently infected vaccinated animals.Likewise, no of IFN-gamma producing cell response against CSFV or PHA was observed.

View Article: PubMed Central - PubMed

Affiliation: Centre de Recerca en Sanitat Animal (CReSA)-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. sara.munoz@cresa.uab.es.

ABSTRACT
Classical swine fever (CSF) causes major losses in pig farming, with various degrees of disease severity. Efficient live attenuated vaccines against classical swine fever virus (CSFV) are used routinely in endemic countries. However, despite intensive vaccination programs in these areas for more than 20 years, CSF has not been eradicated. Molecular epidemiology studies in these regions suggests that the virus circulating in the field has evolved under the positive selection pressure exerted by the immune response to the vaccine, leading to new attenuated viral variants. Recent work by our group demonstrated that a high proportion of persistently infected piglets can be generated by early postnatal infection with low and moderately virulent CSFV strains. Here, we studied the immune response to a hog cholera lapinised virus vaccine (HCLV), C-strain, in six-week-old persistently infected pigs following post-natal infection. CSFV-negative pigs were vaccinated as controls. The humoral and interferon gamma responses as well as the CSFV RNA loads were monitored for 21 days post-vaccination. No vaccine viral RNA was detected in the serum samples and tonsils from CSFV postnatally persistently infected pigs for 21 days post-vaccination. Furthermore, no E2-specific antibody response or neutralising antibody titres were shown in CSFV persistently infected vaccinated animals. Likewise, no of IFN-gamma producing cell response against CSFV or PHA was observed. To our knowledge, this is the first report demonstrating the absence of a response to vaccination in CSFV persistently infected pigs.

No MeSH data available.


Related in: MedlinePlus