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Immunomodulatory effects of preparations from Anthroposophical Medicine for parenteral use.

Gründemann C, Diegel C, Sauer B, Garcia-Käufer M, Huber R - BMC Complement Altern Med (2015)

Bottom Line: We wanted to investigate effects of a selection of AM medications for parenteral use in cell-based systems in vitro.Rosmarinus officinale 5%, but not the other preparations, dose-dependently inhibited the maturation of immature DCs, reduced secretion of IL-10 and IL-12p70 and slightly inhibited proliferation of allogeneic CD4(+) T-lymphocytes in coculture experiments with DCs.Rosmarinus officinale 5% has immunosuppressive properties on key functions of the immune system which propose further investigation.

View Article: PubMed Central - PubMed

Affiliation: Center for Complementary Medicine, Institute for Environmental Health Sciences, University Medical Center Freiburg, Breisacher Str. 115B, 79106, Freiburg, Germany. carsten.gruendemann@uniklinik-freiburg.de.

ABSTRACT

Background: Preparations from anthroposophical medicine (AM) are clinically used to treat inflammatory disorders. We wanted to investigate effects of a selection of AM medications for parenteral use in cell-based systems in vitro.

Methods: Colchicum officinale tuber D3, Mandragora D3, Rosmarinus officinale 5% and Bryophyllum 5% were selected for the experiments. Induction of apoptosis and necrosis (human lymphocytes and dendritic cells [DCs]) and proliferation of lymphocytes as well as maturation (expression of CD14, CD83 and CD86) and cytokine secretion (IL-10, IL12p70) of DCs were analyzed. Furthermore, proliferation of allogeneic human T lymphocytes was investigated in vitro in coculture experiments using mature DCs in comparison to controls.

Results: The respective preparations did not induce apoptosis or necrosis in lymphocytes or DCs. Lymphocyte proliferation was dose-dependently reduced by Colchicum officinale tuber D3 while the viability was unchanged. Rosmarinus officinale 5%, but not the other preparations, dose-dependently inhibited the maturation of immature DCs, reduced secretion of IL-10 and IL-12p70 and slightly inhibited proliferation of allogeneic CD4(+) T-lymphocytes in coculture experiments with DCs.

Conclusion: The selected preparations from AM for parenteral use are nontoxic to lymphocytes and DCs. Rosmarinus officinale 5% has immunosuppressive properties on key functions of the immune system which propose further investigation.

No MeSH data available.


Related in: MedlinePlus

Effects of Rosmarinus injectable preparation on cytokine release of DCs. Secretion of IL-10 (a) and IL-12p70 (b) of iDCs or mDCs alone or in the presence of different concentrations of Rosmarinus (0.25-1 mg/mL) were analyzed in the supernatants of the cells using ELISA-based flow cytometry. Results are summarized from four to six independent experiments, and data are presented as mean ± SD. The detection levels were 2 pg/mL (IL-10) and 4 pg/mL (IL-12) respectively. The asterisks represent significant differences from untreated mDCs alone (**P < 0.01; paired t-test)
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Fig4: Effects of Rosmarinus injectable preparation on cytokine release of DCs. Secretion of IL-10 (a) and IL-12p70 (b) of iDCs or mDCs alone or in the presence of different concentrations of Rosmarinus (0.25-1 mg/mL) were analyzed in the supernatants of the cells using ELISA-based flow cytometry. Results are summarized from four to six independent experiments, and data are presented as mean ± SD. The detection levels were 2 pg/mL (IL-10) and 4 pg/mL (IL-12) respectively. The asterisks represent significant differences from untreated mDCs alone (**P < 0.01; paired t-test)

Mentions: We cultured DCs in the presence of different concentrations of Rosmarinus (1:2000–1:500 corresponding to 0.25- 1 mg/mL) to see whether there is an influence on IL-10 and IL12p70 secretion of DCs (Fig. 4). iDCs per se did not secrete IL-10 (Fig. 4a; no detection) and IL-12 (Fig. 4b; 12 % ± 25) in comparison to mDCs (=100 %). The presence of the Rosmarinus preparation reduced the release of IL-10 (Fig. 4a) (0.25 mg/mL: 115 % ± 37; 0.5 mg/mL: 99 % ± 40; 1.0 mg/mL: 64 % ± 21) and IL12p70 (Fig. 4b) (0.25 mg/mL: 115 % ± 30; 0.5 mg/mL: 89 % ± 37; 1.0 mg/mL: 58 % ± 56) which shows that not only maturation but also the activation status of DCs is modulated by the Rosmarinus extract.Fig. 4


Immunomodulatory effects of preparations from Anthroposophical Medicine for parenteral use.

Gründemann C, Diegel C, Sauer B, Garcia-Käufer M, Huber R - BMC Complement Altern Med (2015)

Effects of Rosmarinus injectable preparation on cytokine release of DCs. Secretion of IL-10 (a) and IL-12p70 (b) of iDCs or mDCs alone or in the presence of different concentrations of Rosmarinus (0.25-1 mg/mL) were analyzed in the supernatants of the cells using ELISA-based flow cytometry. Results are summarized from four to six independent experiments, and data are presented as mean ± SD. The detection levels were 2 pg/mL (IL-10) and 4 pg/mL (IL-12) respectively. The asterisks represent significant differences from untreated mDCs alone (**P < 0.01; paired t-test)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4496844&req=5

Fig4: Effects of Rosmarinus injectable preparation on cytokine release of DCs. Secretion of IL-10 (a) and IL-12p70 (b) of iDCs or mDCs alone or in the presence of different concentrations of Rosmarinus (0.25-1 mg/mL) were analyzed in the supernatants of the cells using ELISA-based flow cytometry. Results are summarized from four to six independent experiments, and data are presented as mean ± SD. The detection levels were 2 pg/mL (IL-10) and 4 pg/mL (IL-12) respectively. The asterisks represent significant differences from untreated mDCs alone (**P < 0.01; paired t-test)
Mentions: We cultured DCs in the presence of different concentrations of Rosmarinus (1:2000–1:500 corresponding to 0.25- 1 mg/mL) to see whether there is an influence on IL-10 and IL12p70 secretion of DCs (Fig. 4). iDCs per se did not secrete IL-10 (Fig. 4a; no detection) and IL-12 (Fig. 4b; 12 % ± 25) in comparison to mDCs (=100 %). The presence of the Rosmarinus preparation reduced the release of IL-10 (Fig. 4a) (0.25 mg/mL: 115 % ± 37; 0.5 mg/mL: 99 % ± 40; 1.0 mg/mL: 64 % ± 21) and IL12p70 (Fig. 4b) (0.25 mg/mL: 115 % ± 30; 0.5 mg/mL: 89 % ± 37; 1.0 mg/mL: 58 % ± 56) which shows that not only maturation but also the activation status of DCs is modulated by the Rosmarinus extract.Fig. 4

Bottom Line: We wanted to investigate effects of a selection of AM medications for parenteral use in cell-based systems in vitro.Rosmarinus officinale 5%, but not the other preparations, dose-dependently inhibited the maturation of immature DCs, reduced secretion of IL-10 and IL-12p70 and slightly inhibited proliferation of allogeneic CD4(+) T-lymphocytes in coculture experiments with DCs.Rosmarinus officinale 5% has immunosuppressive properties on key functions of the immune system which propose further investigation.

View Article: PubMed Central - PubMed

Affiliation: Center for Complementary Medicine, Institute for Environmental Health Sciences, University Medical Center Freiburg, Breisacher Str. 115B, 79106, Freiburg, Germany. carsten.gruendemann@uniklinik-freiburg.de.

ABSTRACT

Background: Preparations from anthroposophical medicine (AM) are clinically used to treat inflammatory disorders. We wanted to investigate effects of a selection of AM medications for parenteral use in cell-based systems in vitro.

Methods: Colchicum officinale tuber D3, Mandragora D3, Rosmarinus officinale 5% and Bryophyllum 5% were selected for the experiments. Induction of apoptosis and necrosis (human lymphocytes and dendritic cells [DCs]) and proliferation of lymphocytes as well as maturation (expression of CD14, CD83 and CD86) and cytokine secretion (IL-10, IL12p70) of DCs were analyzed. Furthermore, proliferation of allogeneic human T lymphocytes was investigated in vitro in coculture experiments using mature DCs in comparison to controls.

Results: The respective preparations did not induce apoptosis or necrosis in lymphocytes or DCs. Lymphocyte proliferation was dose-dependently reduced by Colchicum officinale tuber D3 while the viability was unchanged. Rosmarinus officinale 5%, but not the other preparations, dose-dependently inhibited the maturation of immature DCs, reduced secretion of IL-10 and IL-12p70 and slightly inhibited proliferation of allogeneic CD4(+) T-lymphocytes in coculture experiments with DCs.

Conclusion: The selected preparations from AM for parenteral use are nontoxic to lymphocytes and DCs. Rosmarinus officinale 5% has immunosuppressive properties on key functions of the immune system which propose further investigation.

No MeSH data available.


Related in: MedlinePlus