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Introduction to Oxidative Stress in Biomedical and Biological Research.

Breitenbach M, Eckl P - Biomolecules (2015)

Bottom Line: Oxidative stress is now a well-researched area with thousands of new articles appearing every year.We want to give the reader here an overview of the topics in biomedical and basic oxidative stress research which are covered by the authors of this thematic issue.We also want to give the newcomer a short introduction into some of the basic concepts, definitions and analytical procedures used in this field.

View Article: PubMed Central - PubMed

ABSTRACT
Oxidative stress is now a well-researched area with thousands of new articles appearing every year. We want to give the reader here an overview of the topics in biomedical and basic oxidative stress research which are covered by the authors of this thematic issue. We also want to give the newcomer a short introduction into some of the basic concepts, definitions and analytical procedures used in this field.

Show MeSH
Visualization of H2O2 in subcellular compartments. (A) Neonatal rat ventricular cardiomyocytes transfected with the genetically encoded mitochondria targeted H2O2 sensor HyPermito Mitochondrial localization is confirmed by colocalization with the mitochondrial dye Mitotracker Red. Cells were analyzed after monoamine oxidase ativation with dopamine; (B) Neonatal cardiomyocytes expressing cytosol-targeted HyPercytosol before and after addition of 100 µM H2O2. Source: Kaludercic et al., Frontiers in Physiology, 5, article 285 (2014) with permission.
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biomolecules-05-01169-f002: Visualization of H2O2 in subcellular compartments. (A) Neonatal rat ventricular cardiomyocytes transfected with the genetically encoded mitochondria targeted H2O2 sensor HyPermito Mitochondrial localization is confirmed by colocalization with the mitochondrial dye Mitotracker Red. Cells were analyzed after monoamine oxidase ativation with dopamine; (B) Neonatal cardiomyocytes expressing cytosol-targeted HyPercytosol before and after addition of 100 µM H2O2. Source: Kaludercic et al., Frontiers in Physiology, 5, article 285 (2014) with permission.

Mentions: Another and very important series of methodological advances in the last few years are redox sensitive fluorescent tags or dyes that enable to study the localization and quantification of redox active compounds, and in fact the redox potential in different subcellular compartments in vivo [21,26,27,28]. These studies make it very clear that redox-active micro-domains exist in the known subcellular compartments, such as mitochondria and the ER, and even in the cytoplasm of cells. A whole series of protein reporters based on the bacterial H2O2 sensitive HyPer protein domain has been developed (reviewed in [26]). An alternative method uses roGFP and related constructs to measure the redox potential in subcellular domains [29,30]. Figure 2 shows an example of the H2O2 detected by mitochondrial HyPer in heart muscle cells metabolically stimulated by dopamine.


Introduction to Oxidative Stress in Biomedical and Biological Research.

Breitenbach M, Eckl P - Biomolecules (2015)

Visualization of H2O2 in subcellular compartments. (A) Neonatal rat ventricular cardiomyocytes transfected with the genetically encoded mitochondria targeted H2O2 sensor HyPermito Mitochondrial localization is confirmed by colocalization with the mitochondrial dye Mitotracker Red. Cells were analyzed after monoamine oxidase ativation with dopamine; (B) Neonatal cardiomyocytes expressing cytosol-targeted HyPercytosol before and after addition of 100 µM H2O2. Source: Kaludercic et al., Frontiers in Physiology, 5, article 285 (2014) with permission.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496716&req=5

biomolecules-05-01169-f002: Visualization of H2O2 in subcellular compartments. (A) Neonatal rat ventricular cardiomyocytes transfected with the genetically encoded mitochondria targeted H2O2 sensor HyPermito Mitochondrial localization is confirmed by colocalization with the mitochondrial dye Mitotracker Red. Cells were analyzed after monoamine oxidase ativation with dopamine; (B) Neonatal cardiomyocytes expressing cytosol-targeted HyPercytosol before and after addition of 100 µM H2O2. Source: Kaludercic et al., Frontiers in Physiology, 5, article 285 (2014) with permission.
Mentions: Another and very important series of methodological advances in the last few years are redox sensitive fluorescent tags or dyes that enable to study the localization and quantification of redox active compounds, and in fact the redox potential in different subcellular compartments in vivo [21,26,27,28]. These studies make it very clear that redox-active micro-domains exist in the known subcellular compartments, such as mitochondria and the ER, and even in the cytoplasm of cells. A whole series of protein reporters based on the bacterial H2O2 sensitive HyPer protein domain has been developed (reviewed in [26]). An alternative method uses roGFP and related constructs to measure the redox potential in subcellular domains [29,30]. Figure 2 shows an example of the H2O2 detected by mitochondrial HyPer in heart muscle cells metabolically stimulated by dopamine.

Bottom Line: Oxidative stress is now a well-researched area with thousands of new articles appearing every year.We want to give the reader here an overview of the topics in biomedical and basic oxidative stress research which are covered by the authors of this thematic issue.We also want to give the newcomer a short introduction into some of the basic concepts, definitions and analytical procedures used in this field.

View Article: PubMed Central - PubMed

ABSTRACT
Oxidative stress is now a well-researched area with thousands of new articles appearing every year. We want to give the reader here an overview of the topics in biomedical and basic oxidative stress research which are covered by the authors of this thematic issue. We also want to give the newcomer a short introduction into some of the basic concepts, definitions and analytical procedures used in this field.

Show MeSH