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Asymmetric lipid membranes: towards more realistic model systems.

Marquardt D, Geier B, Pabst G - Membranes (Basel) (2015)

Bottom Line: Despite the ubiquity of transbilayer asymmetry in natural cell membranes, the vast majority of existing research has utilized chemically well-defined symmetric liposomes, where the inner and outer bilayer leaflets have the same composition.Here, we review various aspects of asymmetry in nature and in model systems in anticipation for the next phase of model membrane studies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biosciences, Biophysics Division, University of Graz, NAWI Graz, Humboldtstr 50/III, Graz, 8010, Austria. drew.marquardt@uni-graz.at.

ABSTRACT
Despite the ubiquity of transbilayer asymmetry in natural cell membranes, the vast majority of existing research has utilized chemically well-defined symmetric liposomes, where the inner and outer bilayer leaflets have the same composition. Here, we review various aspects of asymmetry in nature and in model systems in anticipation for the next phase of model membrane studies.

No MeSH data available.


Related in: MedlinePlus

Schematic of asymmetric vesicle construction methods. The left is a schematic of cyclodextrin-mediated lipid exchange of the outer membrane leaflet [10]. The right-hand scheme is the engineering of asymmetric vesicles generated by two independently prepared monolayers [54].
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membranes-05-00180-f004: Schematic of asymmetric vesicle construction methods. The left is a schematic of cyclodextrin-mediated lipid exchange of the outer membrane leaflet [10]. The right-hand scheme is the engineering of asymmetric vesicles generated by two independently prepared monolayers [54].

Mentions: Most promising in this respect appears to be important progress made in the laboratory of Erwin London (Stony Brook, NY, USA) using cyclodextrin-mediated lipid exchange, as depicted in Figure 4 (left) [10,28,57,58,59]. Recently, the protocol has been adapted for the construction of asymmetric supported bilayers [60]. The technique can be applied to construct free-floating asymmetric lipid vesicles with various headgroup and acyl chain compositions containing Chol without the removal of Chol from the model membrane by cyclodextrin [58,59,61]. This has opened a new window to several biophysical studies, including the reconstitution of membrane proteins, as discussed further below.


Asymmetric lipid membranes: towards more realistic model systems.

Marquardt D, Geier B, Pabst G - Membranes (Basel) (2015)

Schematic of asymmetric vesicle construction methods. The left is a schematic of cyclodextrin-mediated lipid exchange of the outer membrane leaflet [10]. The right-hand scheme is the engineering of asymmetric vesicles generated by two independently prepared monolayers [54].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496639&req=5

membranes-05-00180-f004: Schematic of asymmetric vesicle construction methods. The left is a schematic of cyclodextrin-mediated lipid exchange of the outer membrane leaflet [10]. The right-hand scheme is the engineering of asymmetric vesicles generated by two independently prepared monolayers [54].
Mentions: Most promising in this respect appears to be important progress made in the laboratory of Erwin London (Stony Brook, NY, USA) using cyclodextrin-mediated lipid exchange, as depicted in Figure 4 (left) [10,28,57,58,59]. Recently, the protocol has been adapted for the construction of asymmetric supported bilayers [60]. The technique can be applied to construct free-floating asymmetric lipid vesicles with various headgroup and acyl chain compositions containing Chol without the removal of Chol from the model membrane by cyclodextrin [58,59,61]. This has opened a new window to several biophysical studies, including the reconstitution of membrane proteins, as discussed further below.

Bottom Line: Despite the ubiquity of transbilayer asymmetry in natural cell membranes, the vast majority of existing research has utilized chemically well-defined symmetric liposomes, where the inner and outer bilayer leaflets have the same composition.Here, we review various aspects of asymmetry in nature and in model systems in anticipation for the next phase of model membrane studies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biosciences, Biophysics Division, University of Graz, NAWI Graz, Humboldtstr 50/III, Graz, 8010, Austria. drew.marquardt@uni-graz.at.

ABSTRACT
Despite the ubiquity of transbilayer asymmetry in natural cell membranes, the vast majority of existing research has utilized chemically well-defined symmetric liposomes, where the inner and outer bilayer leaflets have the same composition. Here, we review various aspects of asymmetry in nature and in model systems in anticipation for the next phase of model membrane studies.

No MeSH data available.


Related in: MedlinePlus