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Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection.

Curto M, Krajinski F, Schlereth A, Rubiales D - Front Plant Sci (2015)

Bottom Line: Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown.Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense.Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, Institute for Sustainable Agriculture, Spanish National Research Council Córdoba, Spain.

ABSTRACT
Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

No MeSH data available.


Related in: MedlinePlus

Most differentially expressed TF families in SA1306 compared to Parabinga (−0.7 >F> 0.7) in response toErysiphe pisiinfection. The TF families are represented by circles whose size is proportional to the number of genes in the family. Up-regulation (m ≥ 0.7) is indicated by red arrows; down-regulation (m ≤ −0.7) by green arrows; no differential expression (−0.7 ≤ m ≤ 0.7) are indicate by double red/green arrows; solid lines represent Parabinga (PB), and dotted lines SA1306 (SA). TF families highly up-regulated (F ≥ 1.8) and down-regulated (F ≤ −1.8) are indicated by red and green boxes, respectively. For expanded information, see Table S3.
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Figure 5: Most differentially expressed TF families in SA1306 compared to Parabinga (−0.7 >F> 0.7) in response toErysiphe pisiinfection. The TF families are represented by circles whose size is proportional to the number of genes in the family. Up-regulation (m ≥ 0.7) is indicated by red arrows; down-regulation (m ≤ −0.7) by green arrows; no differential expression (−0.7 ≤ m ≤ 0.7) are indicate by double red/green arrows; solid lines represent Parabinga (PB), and dotted lines SA1306 (SA). TF families highly up-regulated (F ≥ 1.8) and down-regulated (F ≤ −1.8) are indicated by red and green boxes, respectively. For expanded information, see Table S3.

Mentions: Interestingly, 48 genes were differentially expressed in SA1306 compared to Parabinga (P < 0.05; −0.7 > F > 0.7) in response to E. pisi infection, and they belong to 25 TF families (Figure 5, Table S3). Among them, the most represented TF families are HD-like, C2H2 (Zn), AP2/EREBP, MYB/HD-like, and HD/HD, which comprise approximately half of the differentially expressed genes. The bHLH, C2C2/DOF (CYS2-CYS2/DNA-BINDING ONE ZINC FINGER) (Zn), HD-ZIP (HD-ZINC-REGULATED TRANSPORTER), MYB, NAC, and SBP TF families were also well represented (≈24%) and the rest of TF families were poorly represented (Figure 5). Eleven of 25 families include genes with greater transcript accumulation (F > 0.7) in SA1306 compared to Parabinga (Figure 5). Among them, the genes belonging to the ARID, WRKY family/WRKY, C2C2 (Zn)/DOF, SBP, HD-like, MADS, and CCHC (Zn) families were up-regulated around two fold in SA1306 compared to Parabinga. Meanwhile only three families, TTF-type (Zn), bHLH, and HMG, include genes that were down-regulated (F ≤ −2) in SA1306 compared to Parabinga (Figure 5). Generally, families HD-ZIP and NAC include genes that were induced in both genotypes. However, the AP2/ERBP family was lightly up-regulated in SA1306 and almost not regulated in Parabinga (Table S3).


Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection.

Curto M, Krajinski F, Schlereth A, Rubiales D - Front Plant Sci (2015)

Most differentially expressed TF families in SA1306 compared to Parabinga (−0.7 >F> 0.7) in response toErysiphe pisiinfection. The TF families are represented by circles whose size is proportional to the number of genes in the family. Up-regulation (m ≥ 0.7) is indicated by red arrows; down-regulation (m ≤ −0.7) by green arrows; no differential expression (−0.7 ≤ m ≤ 0.7) are indicate by double red/green arrows; solid lines represent Parabinga (PB), and dotted lines SA1306 (SA). TF families highly up-regulated (F ≥ 1.8) and down-regulated (F ≤ −1.8) are indicated by red and green boxes, respectively. For expanded information, see Table S3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496563&req=5

Figure 5: Most differentially expressed TF families in SA1306 compared to Parabinga (−0.7 >F> 0.7) in response toErysiphe pisiinfection. The TF families are represented by circles whose size is proportional to the number of genes in the family. Up-regulation (m ≥ 0.7) is indicated by red arrows; down-regulation (m ≤ −0.7) by green arrows; no differential expression (−0.7 ≤ m ≤ 0.7) are indicate by double red/green arrows; solid lines represent Parabinga (PB), and dotted lines SA1306 (SA). TF families highly up-regulated (F ≥ 1.8) and down-regulated (F ≤ −1.8) are indicated by red and green boxes, respectively. For expanded information, see Table S3.
Mentions: Interestingly, 48 genes were differentially expressed in SA1306 compared to Parabinga (P < 0.05; −0.7 > F > 0.7) in response to E. pisi infection, and they belong to 25 TF families (Figure 5, Table S3). Among them, the most represented TF families are HD-like, C2H2 (Zn), AP2/EREBP, MYB/HD-like, and HD/HD, which comprise approximately half of the differentially expressed genes. The bHLH, C2C2/DOF (CYS2-CYS2/DNA-BINDING ONE ZINC FINGER) (Zn), HD-ZIP (HD-ZINC-REGULATED TRANSPORTER), MYB, NAC, and SBP TF families were also well represented (≈24%) and the rest of TF families were poorly represented (Figure 5). Eleven of 25 families include genes with greater transcript accumulation (F > 0.7) in SA1306 compared to Parabinga (Figure 5). Among them, the genes belonging to the ARID, WRKY family/WRKY, C2C2 (Zn)/DOF, SBP, HD-like, MADS, and CCHC (Zn) families were up-regulated around two fold in SA1306 compared to Parabinga. Meanwhile only three families, TTF-type (Zn), bHLH, and HMG, include genes that were down-regulated (F ≤ −2) in SA1306 compared to Parabinga (Figure 5). Generally, families HD-ZIP and NAC include genes that were induced in both genotypes. However, the AP2/ERBP family was lightly up-regulated in SA1306 and almost not regulated in Parabinga (Table S3).

Bottom Line: Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown.Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense.Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, Institute for Sustainable Agriculture, Spanish National Research Council Córdoba, Spain.

ABSTRACT
Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

No MeSH data available.


Related in: MedlinePlus