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Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection.

Curto M, Krajinski F, Schlereth A, Rubiales D - Front Plant Sci (2015)

Bottom Line: Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown.Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense.Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, Institute for Sustainable Agriculture, Spanish National Research Council Córdoba, Spain.

ABSTRACT
Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of TF genes. Percentages and numbers (into the brackets) of up-regulated (in red), down-regulated (in green), and no differential expressed (in gray) genes detected in Parabinga (PB) and SA1306 (SA) for each of the 10 clusters (groups GI–GX). Top on each cluster the total number of TF regulated genes is indicated. Additional information is available in Table 1.
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Figure 3: Expression profiles of TF genes. Percentages and numbers (into the brackets) of up-regulated (in red), down-regulated (in green), and no differential expressed (in gray) genes detected in Parabinga (PB) and SA1306 (SA) for each of the 10 clusters (groups GI–GX). Top on each cluster the total number of TF regulated genes is indicated. Additional information is available in Table 1.

Mentions: Heat map expression profiles clustered. Additional information is given in Figures 2, 3.


Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection.

Curto M, Krajinski F, Schlereth A, Rubiales D - Front Plant Sci (2015)

Expression profiles of TF genes. Percentages and numbers (into the brackets) of up-regulated (in red), down-regulated (in green), and no differential expressed (in gray) genes detected in Parabinga (PB) and SA1306 (SA) for each of the 10 clusters (groups GI–GX). Top on each cluster the total number of TF regulated genes is indicated. Additional information is available in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496563&req=5

Figure 3: Expression profiles of TF genes. Percentages and numbers (into the brackets) of up-regulated (in red), down-regulated (in green), and no differential expressed (in gray) genes detected in Parabinga (PB) and SA1306 (SA) for each of the 10 clusters (groups GI–GX). Top on each cluster the total number of TF regulated genes is indicated. Additional information is available in Table 1.
Mentions: Heat map expression profiles clustered. Additional information is given in Figures 2, 3.

Bottom Line: Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown.Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense.Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, Institute for Sustainable Agriculture, Spanish National Research Council Córdoba, Spain.

ABSTRACT
Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

No MeSH data available.


Related in: MedlinePlus