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Iron deficiency in barley plants: phytosiderophore release, iron translocation, and DNA methylation.

Bocchini M, Bartucca ML, Ciancaleoni S, Mimmo T, Cesco S, Pii Y, Albertini E, Del Buono D - Front Plant Sci (2015)

Bottom Line: Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found.Eleven DNA bands differently methylated were found in starved plants.A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural, Food and Environmental Sciences, University of Perugia Perugia, Italy.

ABSTRACT
All living organisms require iron (Fe) to carry out many crucial metabolic pathways. Despite its high concentrations in the geosphere, Fe bio-availability to plant roots can be very scarce. To cope with Fe shortage, plants can activate different strategies. For these reasons, we investigated Fe deficient Hordeum vulgare L. plants by monitoring growth, phytosiderophores (PS) release, iron content, and translocation, and DNA methylation, with respect to Fe sufficient ones. Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found. Experiments on DNA methylation highlighted significant differences between fully and hemy-methylated sequences in Fe deficient plants, with respect to Fe sufficient plants. Eleven DNA bands differently methylated were found in starved plants. Of these, five sequences showed significant alignment to barley genes encoding for a glucosyltransferase, a putative acyl carrier protein, a peroxidase, a β-glucosidase and a transcription factor containing a Homeodomin. A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition. In fact, Fe absorption and root to shoot translocation capacities were impaired. In addition, resupplied barley showed DNA methylation/demethylation patterns very similar to that of barley grown in Fe deprivation. This last finding is very encouraging because it indicates as these variations/modifications could be transmitted to progenies.

No MeSH data available.


Related in: MedlinePlus

Phytosiderophores release (μmol g−1 RFW) from barley plants grown under Fe-deficiency at 9, 12, 13, 15, and 19 DAS.
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Figure 2: Phytosiderophores release (μmol g−1 RFW) from barley plants grown under Fe-deficiency at 9, 12, 13, 15, and 19 DAS.

Mentions: Root exudates were collected from barley plants grown under Fe deficiency in hydroponic solutions. The quantification of exudates was started 8 DAS, but, at this time, the concentration of these organic compounds was scarce and far below the limit of detection of the method (30 μM). Starting from 9 DAS, Fe deficient plants began to exude higher concentrations of PSs, and this exudation pattern was found to be quite constant until 12 DAS (Figure 2). On the other hand, starting from 13 DAS the amount of extruded PSs by the plants strongly increased. In particular, at 13, 15, and 19 DAS Fe deficient barley exuded 1.03, 1.00, and 1.10 μmol g−1 RFW, respectively.


Iron deficiency in barley plants: phytosiderophore release, iron translocation, and DNA methylation.

Bocchini M, Bartucca ML, Ciancaleoni S, Mimmo T, Cesco S, Pii Y, Albertini E, Del Buono D - Front Plant Sci (2015)

Phytosiderophores release (μmol g−1 RFW) from barley plants grown under Fe-deficiency at 9, 12, 13, 15, and 19 DAS.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496560&req=5

Figure 2: Phytosiderophores release (μmol g−1 RFW) from barley plants grown under Fe-deficiency at 9, 12, 13, 15, and 19 DAS.
Mentions: Root exudates were collected from barley plants grown under Fe deficiency in hydroponic solutions. The quantification of exudates was started 8 DAS, but, at this time, the concentration of these organic compounds was scarce and far below the limit of detection of the method (30 μM). Starting from 9 DAS, Fe deficient plants began to exude higher concentrations of PSs, and this exudation pattern was found to be quite constant until 12 DAS (Figure 2). On the other hand, starting from 13 DAS the amount of extruded PSs by the plants strongly increased. In particular, at 13, 15, and 19 DAS Fe deficient barley exuded 1.03, 1.00, and 1.10 μmol g−1 RFW, respectively.

Bottom Line: Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found.Eleven DNA bands differently methylated were found in starved plants.A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural, Food and Environmental Sciences, University of Perugia Perugia, Italy.

ABSTRACT
All living organisms require iron (Fe) to carry out many crucial metabolic pathways. Despite its high concentrations in the geosphere, Fe bio-availability to plant roots can be very scarce. To cope with Fe shortage, plants can activate different strategies. For these reasons, we investigated Fe deficient Hordeum vulgare L. plants by monitoring growth, phytosiderophores (PS) release, iron content, and translocation, and DNA methylation, with respect to Fe sufficient ones. Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found. Experiments on DNA methylation highlighted significant differences between fully and hemy-methylated sequences in Fe deficient plants, with respect to Fe sufficient plants. Eleven DNA bands differently methylated were found in starved plants. Of these, five sequences showed significant alignment to barley genes encoding for a glucosyltransferase, a putative acyl carrier protein, a peroxidase, a β-glucosidase and a transcription factor containing a Homeodomin. A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition. In fact, Fe absorption and root to shoot translocation capacities were impaired. In addition, resupplied barley showed DNA methylation/demethylation patterns very similar to that of barley grown in Fe deprivation. This last finding is very encouraging because it indicates as these variations/modifications could be transmitted to progenies.

No MeSH data available.


Related in: MedlinePlus