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Iron deficiency in barley plants: phytosiderophore release, iron translocation, and DNA methylation.

Bocchini M, Bartucca ML, Ciancaleoni S, Mimmo T, Cesco S, Pii Y, Albertini E, Del Buono D - Front Plant Sci (2015)

Bottom Line: Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found.Eleven DNA bands differently methylated were found in starved plants.A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural, Food and Environmental Sciences, University of Perugia Perugia, Italy.

ABSTRACT
All living organisms require iron (Fe) to carry out many crucial metabolic pathways. Despite its high concentrations in the geosphere, Fe bio-availability to plant roots can be very scarce. To cope with Fe shortage, plants can activate different strategies. For these reasons, we investigated Fe deficient Hordeum vulgare L. plants by monitoring growth, phytosiderophores (PS) release, iron content, and translocation, and DNA methylation, with respect to Fe sufficient ones. Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found. Experiments on DNA methylation highlighted significant differences between fully and hemy-methylated sequences in Fe deficient plants, with respect to Fe sufficient plants. Eleven DNA bands differently methylated were found in starved plants. Of these, five sequences showed significant alignment to barley genes encoding for a glucosyltransferase, a putative acyl carrier protein, a peroxidase, a β-glucosidase and a transcription factor containing a Homeodomin. A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition. In fact, Fe absorption and root to shoot translocation capacities were impaired. In addition, resupplied barley showed DNA methylation/demethylation patterns very similar to that of barley grown in Fe deprivation. This last finding is very encouraging because it indicates as these variations/modifications could be transmitted to progenies.

No MeSH data available.


Related in: MedlinePlus

Chlorophyll concentration in barley shoots grown under Fe sufficiency (+Fe) and Fe-deficiency (−Fe) conditions, at 9, 12, 13, 15, and 19 DAS. At 9 DAS the SPAD was measured at the first leaves, thereafter, it was recordered on the second leaves.
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Figure 1: Chlorophyll concentration in barley shoots grown under Fe sufficiency (+Fe) and Fe-deficiency (−Fe) conditions, at 9, 12, 13, 15, and 19 DAS. At 9 DAS the SPAD was measured at the first leaves, thereafter, it was recordered on the second leaves.

Mentions: Changes in chlorophyll contents in response to the Fe starvation were assessed in Fe deficient leaves of barley by a SPAD meter (Figure 1). The leaf chlorophyll level was significantly reduced in the Fe deficient plants at 13, 15, and 19 days after this nutrient deprivation and the reductions were of 16.3, 27.4, and 34.5%, respectively.


Iron deficiency in barley plants: phytosiderophore release, iron translocation, and DNA methylation.

Bocchini M, Bartucca ML, Ciancaleoni S, Mimmo T, Cesco S, Pii Y, Albertini E, Del Buono D - Front Plant Sci (2015)

Chlorophyll concentration in barley shoots grown under Fe sufficiency (+Fe) and Fe-deficiency (−Fe) conditions, at 9, 12, 13, 15, and 19 DAS. At 9 DAS the SPAD was measured at the first leaves, thereafter, it was recordered on the second leaves.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496560&req=5

Figure 1: Chlorophyll concentration in barley shoots grown under Fe sufficiency (+Fe) and Fe-deficiency (−Fe) conditions, at 9, 12, 13, 15, and 19 DAS. At 9 DAS the SPAD was measured at the first leaves, thereafter, it was recordered on the second leaves.
Mentions: Changes in chlorophyll contents in response to the Fe starvation were assessed in Fe deficient leaves of barley by a SPAD meter (Figure 1). The leaf chlorophyll level was significantly reduced in the Fe deficient plants at 13, 15, and 19 days after this nutrient deprivation and the reductions were of 16.3, 27.4, and 34.5%, respectively.

Bottom Line: Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found.Eleven DNA bands differently methylated were found in starved plants.A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural, Food and Environmental Sciences, University of Perugia Perugia, Italy.

ABSTRACT
All living organisms require iron (Fe) to carry out many crucial metabolic pathways. Despite its high concentrations in the geosphere, Fe bio-availability to plant roots can be very scarce. To cope with Fe shortage, plants can activate different strategies. For these reasons, we investigated Fe deficient Hordeum vulgare L. plants by monitoring growth, phytosiderophores (PS) release, iron content, and translocation, and DNA methylation, with respect to Fe sufficient ones. Reductions of plant growth, roots to shoots Fe translocation, and increases in PS release were found. Experiments on DNA methylation highlighted significant differences between fully and hemy-methylated sequences in Fe deficient plants, with respect to Fe sufficient plants. Eleven DNA bands differently methylated were found in starved plants. Of these, five sequences showed significant alignment to barley genes encoding for a glucosyltransferase, a putative acyl carrier protein, a peroxidase, a β-glucosidase and a transcription factor containing a Homeodomin. A resupply experiment was carried out on starved barley re-fed at 13 days after sowing (DAS), and it showed that plants did not recover after Fe addition. In fact, Fe absorption and root to shoot translocation capacities were impaired. In addition, resupplied barley showed DNA methylation/demethylation patterns very similar to that of barley grown in Fe deprivation. This last finding is very encouraging because it indicates as these variations/modifications could be transmitted to progenies.

No MeSH data available.


Related in: MedlinePlus