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The effects of acute hyperinsulinemia on bone metabolism.

Ivaska KK, Heliövaara MK, Ebeling P, Bucci M, Huovinen V, Väänänen HK, Nuutila P, Koistinen HA - Endocr Connect (2015)

Bottom Line: Thirty-three subjects from three separate cohorts (n=8, n=12 and n=13) participated in a euglycaemic hyperinsulinemic clamp study.In conclusion, insulin decreases bone resorption and circulating levels of total OC and ucOC.Insulin has direct effects on bone metabolism in humans and changes in the circulating levels of bone markers can be seen within a few hours after administration of insulin.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and AnatomyInstitute of Biomedicine, University of Turku, FI-20520 Turku, FinlandDepartment of MedicineUniversity of Helsinki and Helsinki University Central Hospital, Helsinki, FinlandTurku PET CentreUniversity of Turku, Turku, FinlandDepartment of RadiologyUniversity of Turku, Turku, FinlandMedical Imaging Centre of Southwest FinlandTurku University Hospital, Turku, FinlandDepartment of EndocrinologyTurku University Hospital, Turku, FinlandAbdominal Center: EndocrinologyUniversity of Helsinki and Helsinki University Central Hospital, Helsinki, FinlandMinerva Foundation Institute for Medical ResearchHelsinki, Finland kaisa.ivaska@utu.fi.

No MeSH data available.


Related in: MedlinePlus

Experimental design. Glucose was clamped at 5 mM and insulin infusion rates were 40 or 72 mU/m2 per min. Timepoints for sample collection are indicated with triangles. Samples were analysed for PINP, CTX, TRAcP5b, TotalOC and ucOC.
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fig1: Experimental design. Glucose was clamped at 5 mM and insulin infusion rates were 40 or 72 mU/m2 per min. Timepoints for sample collection are indicated with triangles. Samples were analysed for PINP, CTX, TRAcP5b, TotalOC and ucOC.

Mentions: The clamp study was initiated after an overnight fast at 0900 h and blood samples were collected at the beginning of the study before infusions (0 min) and at the end of infusions (120 or 240 min, depending on the experimental protocol, Fig. 1). Serum was stored in aliquots at –70 °C until analysis and all samples were analyzed in duplicate in a blinded fashion. Whole body insulin sensitivity was calculated as glucose disposal rate (GDR, or M value; μmol/kg per min) during the last hour of the clamp (22).


The effects of acute hyperinsulinemia on bone metabolism.

Ivaska KK, Heliövaara MK, Ebeling P, Bucci M, Huovinen V, Väänänen HK, Nuutila P, Koistinen HA - Endocr Connect (2015)

Experimental design. Glucose was clamped at 5 mM and insulin infusion rates were 40 or 72 mU/m2 per min. Timepoints for sample collection are indicated with triangles. Samples were analysed for PINP, CTX, TRAcP5b, TotalOC and ucOC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496528&req=5

fig1: Experimental design. Glucose was clamped at 5 mM and insulin infusion rates were 40 or 72 mU/m2 per min. Timepoints for sample collection are indicated with triangles. Samples were analysed for PINP, CTX, TRAcP5b, TotalOC and ucOC.
Mentions: The clamp study was initiated after an overnight fast at 0900 h and blood samples were collected at the beginning of the study before infusions (0 min) and at the end of infusions (120 or 240 min, depending on the experimental protocol, Fig. 1). Serum was stored in aliquots at –70 °C until analysis and all samples were analyzed in duplicate in a blinded fashion. Whole body insulin sensitivity was calculated as glucose disposal rate (GDR, or M value; μmol/kg per min) during the last hour of the clamp (22).

Bottom Line: Thirty-three subjects from three separate cohorts (n=8, n=12 and n=13) participated in a euglycaemic hyperinsulinemic clamp study.In conclusion, insulin decreases bone resorption and circulating levels of total OC and ucOC.Insulin has direct effects on bone metabolism in humans and changes in the circulating levels of bone markers can be seen within a few hours after administration of insulin.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and AnatomyInstitute of Biomedicine, University of Turku, FI-20520 Turku, FinlandDepartment of MedicineUniversity of Helsinki and Helsinki University Central Hospital, Helsinki, FinlandTurku PET CentreUniversity of Turku, Turku, FinlandDepartment of RadiologyUniversity of Turku, Turku, FinlandMedical Imaging Centre of Southwest FinlandTurku University Hospital, Turku, FinlandDepartment of EndocrinologyTurku University Hospital, Turku, FinlandAbdominal Center: EndocrinologyUniversity of Helsinki and Helsinki University Central Hospital, Helsinki, FinlandMinerva Foundation Institute for Medical ResearchHelsinki, Finland kaisa.ivaska@utu.fi.

No MeSH data available.


Related in: MedlinePlus