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Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus

Confocal microscopic examination of skin equivalents. (a) The number of p63 positive cells increased significantly in response to treatment with ACQ compared to the control. In addition, the expression of α6 integrin was also significantly increased but nonspecific staining was observed at the upper epidermis (×200, scale bar is 50 μM).
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fig7: Confocal microscopic examination of skin equivalents. (a) The number of p63 positive cells increased significantly in response to treatment with ACQ compared to the control. In addition, the expression of α6 integrin was also significantly increased but nonspecific staining was observed at the upper epidermis (×200, scale bar is 50 μM).

Mentions: Results showed that epidermal thickness is dramatically increased by ACQ treatment by dose-dependent manner (0.1, 1 μg/mL) (Figure 6). Increased thickness was correlated with the increased number of keratinocytes but differentiation was not affected. In addition, confocal microscopic examination showed increased number of p63 positive cells in ACQ treated samples. Furthermore, integrin α6 staining was definitely increased by ACQ treatment (Figure 7).


Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Confocal microscopic examination of skin equivalents. (a) The number of p63 positive cells increased significantly in response to treatment with ACQ compared to the control. In addition, the expression of α6 integrin was also significantly increased but nonspecific staining was observed at the upper epidermis (×200, scale bar is 50 μM).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4496495&req=5

fig7: Confocal microscopic examination of skin equivalents. (a) The number of p63 positive cells increased significantly in response to treatment with ACQ compared to the control. In addition, the expression of α6 integrin was also significantly increased but nonspecific staining was observed at the upper epidermis (×200, scale bar is 50 μM).
Mentions: Results showed that epidermal thickness is dramatically increased by ACQ treatment by dose-dependent manner (0.1, 1 μg/mL) (Figure 6). Increased thickness was correlated with the increased number of keratinocytes but differentiation was not affected. In addition, confocal microscopic examination showed increased number of p63 positive cells in ACQ treated samples. Furthermore, integrin α6 staining was definitely increased by ACQ treatment (Figure 7).

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus