Limits...
Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus

Decreased DCF staining and increased p63 positive cells in ACQ fed mice. (a) After UVB irradiation, control mice showed strong DCF staining but ACQ fed mice showed weak DCF staining (dashed line means basement membrane; scale bar is 50 μM). (b) Confocal microscopic examination showed that there is strong signal of p63 and integrin α6 in ACQ fed mice compared to control mice. There was nonspecific staining of integrin α6 at the upper dermis (green; p63 staining, red; integrin α6 staining, ×200, scale bar is 50 μM).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4496495&req=5

fig5: Decreased DCF staining and increased p63 positive cells in ACQ fed mice. (a) After UVB irradiation, control mice showed strong DCF staining but ACQ fed mice showed weak DCF staining (dashed line means basement membrane; scale bar is 50 μM). (b) Confocal microscopic examination showed that there is strong signal of p63 and integrin α6 in ACQ fed mice compared to control mice. There was nonspecific staining of integrin α6 at the upper dermis (green; p63 staining, red; integrin α6 staining, ×200, scale bar is 50 μM).

Mentions: To visualize the free radicals, sections were stained with H2DCFA (Sigma-Aldrich, D6883) and results showed strong staining in control mice but these findings were not observed in ACQ treated mice (Figure 5(a)). Furthermore, the number of p63 positive cells was also higher in the ACQ group (Figure 5(b)).


Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Decreased DCF staining and increased p63 positive cells in ACQ fed mice. (a) After UVB irradiation, control mice showed strong DCF staining but ACQ fed mice showed weak DCF staining (dashed line means basement membrane; scale bar is 50 μM). (b) Confocal microscopic examination showed that there is strong signal of p63 and integrin α6 in ACQ fed mice compared to control mice. There was nonspecific staining of integrin α6 at the upper dermis (green; p63 staining, red; integrin α6 staining, ×200, scale bar is 50 μM).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496495&req=5

fig5: Decreased DCF staining and increased p63 positive cells in ACQ fed mice. (a) After UVB irradiation, control mice showed strong DCF staining but ACQ fed mice showed weak DCF staining (dashed line means basement membrane; scale bar is 50 μM). (b) Confocal microscopic examination showed that there is strong signal of p63 and integrin α6 in ACQ fed mice compared to control mice. There was nonspecific staining of integrin α6 at the upper dermis (green; p63 staining, red; integrin α6 staining, ×200, scale bar is 50 μM).
Mentions: To visualize the free radicals, sections were stained with H2DCFA (Sigma-Aldrich, D6883) and results showed strong staining in control mice but these findings were not observed in ACQ treated mice (Figure 5(a)). Furthermore, the number of p63 positive cells was also higher in the ACQ group (Figure 5(b)).

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus