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Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus

Protective effects by ACQ against UV irradiation. Control mice and ACQ fed mice (2 and 4 weeks) were irradiated with 600 mJ/cm2 of UVB. In control mice, epidermis became thinner and apoptotic cells were observed. But in ACQ fed mice, epidermis became thicker and severe damage was not observed. Experiment was repeated 2 times and representative data is shown (×200, scale bar is 50 μM).
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fig3: Protective effects by ACQ against UV irradiation. Control mice and ACQ fed mice (2 and 4 weeks) were irradiated with 600 mJ/cm2 of UVB. In control mice, epidermis became thinner and apoptotic cells were observed. But in ACQ fed mice, epidermis became thicker and severe damage was not observed. Experiment was repeated 2 times and representative data is shown (×200, scale bar is 50 μM).

Mentions: BALB/c nude mice were fed for 2 or 4 weeks. Each group (control mice, UV irradiated mice) consists of 2 mice and experiment was repeated 2 times. One day after UV irradiation, biopsy was taken. In the control group, thinning of epidermis was observed after UVB irradiation. In addition, several apoptotic keratinocytes were observed. But in the ACQ group, epidermis became thicker even after UVB irradiation. In addition, apoptotic cells were barely observed and these findings suggested that ACQ may have protective effects against UV irradiation (Figure 3).


Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Protective effects by ACQ against UV irradiation. Control mice and ACQ fed mice (2 and 4 weeks) were irradiated with 600 mJ/cm2 of UVB. In control mice, epidermis became thinner and apoptotic cells were observed. But in ACQ fed mice, epidermis became thicker and severe damage was not observed. Experiment was repeated 2 times and representative data is shown (×200, scale bar is 50 μM).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496495&req=5

fig3: Protective effects by ACQ against UV irradiation. Control mice and ACQ fed mice (2 and 4 weeks) were irradiated with 600 mJ/cm2 of UVB. In control mice, epidermis became thinner and apoptotic cells were observed. But in ACQ fed mice, epidermis became thicker and severe damage was not observed. Experiment was repeated 2 times and representative data is shown (×200, scale bar is 50 μM).
Mentions: BALB/c nude mice were fed for 2 or 4 weeks. Each group (control mice, UV irradiated mice) consists of 2 mice and experiment was repeated 2 times. One day after UV irradiation, biopsy was taken. In the control group, thinning of epidermis was observed after UVB irradiation. In addition, several apoptotic keratinocytes were observed. But in the ACQ group, epidermis became thicker even after UVB irradiation. In addition, apoptotic cells were barely observed and these findings suggested that ACQ may have protective effects against UV irradiation (Figure 3).

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus