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Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus

Protective effects of ACQ against hydrogen peroxide treatment. (a) Normal human fibroblasts or (b) normal human keratinocytes were incubated overnight with H2O2 after 24 hr treatment of ACQ. (c) Intensity of fluorescence (Lt: fibroblasts, Rt: keratinocytes). Experiment was repeated 2 times and representative data is shown (×200, scale bar is 100 μM).
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fig2: Protective effects of ACQ against hydrogen peroxide treatment. (a) Normal human fibroblasts or (b) normal human keratinocytes were incubated overnight with H2O2 after 24 hr treatment of ACQ. (c) Intensity of fluorescence (Lt: fibroblasts, Rt: keratinocytes). Experiment was repeated 2 times and representative data is shown (×200, scale bar is 100 μM).

Mentions: Cytotoxic or proliferative effects were tested between the concentration of 0~1 μg/mL. In these concentrations, ACQ was not cytotoxic or proliferative to both fibroblasts and keratinocytes (data not shown). To generate free radicals in the cells, H2O2 was treated. Based on cytotoxicity data of ACQ, 50 μM or 100 μM was chosen for fibroblasts and keratinocytes, respectively (data not shown). Nontreated fibroblasts rarely showed positive staining by H2DCFA (D6883, Sigma-Aldrich). In contrast, H2O2 treated cells showed bright positive staining. However, addition of ACQ (0.1, 1 μg/mL) effectively reduced the number of positively stained cells in fibroblasts (Figures 2(a) and 2(c)). Compared to fibroblasts, nontreated normal human keratinocytes showed relatively strong staining by H2DCFA. As expected, H2O2 (100 μM) treatment dramatically increased the staining intensity by and this was decreased by ACQ treatment (Figures 2(b) and 2(c)).


Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells.

Choi HR, Shin JW, Na JI, Nam KM, Lee HS, Park KC - Oxid Med Cell Longev (2015)

Protective effects of ACQ against hydrogen peroxide treatment. (a) Normal human fibroblasts or (b) normal human keratinocytes were incubated overnight with H2O2 after 24 hr treatment of ACQ. (c) Intensity of fluorescence (Lt: fibroblasts, Rt: keratinocytes). Experiment was repeated 2 times and representative data is shown (×200, scale bar is 100 μM).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496495&req=5

fig2: Protective effects of ACQ against hydrogen peroxide treatment. (a) Normal human fibroblasts or (b) normal human keratinocytes were incubated overnight with H2O2 after 24 hr treatment of ACQ. (c) Intensity of fluorescence (Lt: fibroblasts, Rt: keratinocytes). Experiment was repeated 2 times and representative data is shown (×200, scale bar is 100 μM).
Mentions: Cytotoxic or proliferative effects were tested between the concentration of 0~1 μg/mL. In these concentrations, ACQ was not cytotoxic or proliferative to both fibroblasts and keratinocytes (data not shown). To generate free radicals in the cells, H2O2 was treated. Based on cytotoxicity data of ACQ, 50 μM or 100 μM was chosen for fibroblasts and keratinocytes, respectively (data not shown). Nontreated fibroblasts rarely showed positive staining by H2DCFA (D6883, Sigma-Aldrich). In contrast, H2O2 treated cells showed bright positive staining. However, addition of ACQ (0.1, 1 μg/mL) effectively reduced the number of positively stained cells in fibroblasts (Figures 2(a) and 2(c)). Compared to fibroblasts, nontreated normal human keratinocytes showed relatively strong staining by H2DCFA. As expected, H2O2 (100 μM) treatment dramatically increased the staining intensity by and this was decreased by ACQ treatment (Figures 2(b) and 2(c)).

Bottom Line: We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione.But, ACQ showed good protective effects against hydrogen peroxide treatment.To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Republic of Korea.

ABSTRACT
We found that tripeptide "ACQ: alanine-cysteine-glutamine" has significant DPPH scavenging activity compared to that of glutathione. Antioxidant effects of ACQ were tested in in vitro and in vivo models. When treated with H2O2, mock treated fibroblasts and keratinocytes showed strong staining by H2DCFA. But, ACQ showed good protective effects against hydrogen peroxide treatment. When mice were fed for 2 or 4 weeks, similar protective effects were observed. In the control group, epidermis was severely damaged by UV irradiation and apoptotic keratinocytes were observed. There were also numerous TUNEL positive cells. But in the ACQ group, epidermis became thicker and there was no sign of severe damage. Interestingly, the number of p63 cells was also higher in ACQ fed mice. To confirm the stem cell rescuing effects of ACQ, three-dimensional skin samples were constructed. Results showed that ACQ increased the expression of integrin α6 and the number of p63 positive cells. These findings showed that ACQ has good antioxidant activity and may increase stem cell activities by the regulation of integrin α6.

No MeSH data available.


Related in: MedlinePlus