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Hyaluronic Acid (800 kDa) Supplementation of University of Wisconsin Solution Improves Viability of Osteochondral Grafts and Reduces Matrix Metalloproteinase Expression during Cold Preservation.

Yamada T, Uchida K, Onuma K, Inoue G, Aikawa J, Takano S, Sekiguchi H, Fujimaki H, Miyagi M, Takaso M - ScientificWorldJournal (2015)

Bottom Line: The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution.In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT.Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.

ABSTRACT
Osteochondral allografting is a promising option for the treatment of large cartilage defects. However, because the cell viability of osteochondral tissues (OCTs) gradually reduces during storage at 4°C, methods for maintaining the cell viability of fresh OCTs are needed to improve transplantation outcomes. Here, we evaluated whether the supplementation of preservation solution with one of three different molecular weight forms of hyaluronic acid (HA) improved the viability of rat OCTs during long-term cold storage. The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution. In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT. Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA. Although further studies in human OCT are warranted, these findings demonstrate that the use of 800 kDa HA in place of serum may be a suitable approach for the long-term preservation of osteochondral allografts designated for the repair of large cartilage defects in the clinical setting.

No MeSH data available.


Related in: MedlinePlus

Effect of hyaluronic acid on cell viability in OCT after cold preservation for two weeks in UW solution. Data are presented as the mean ± SE (n = 10). aSignificant difference between the UW and UW with HA groups (P < 0.05). bSignificant difference between the nonpreserved OCT and UW and UW with HA groups (P < 0.05). Fresh: nonpreserved OCT samples.
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fig3: Effect of hyaluronic acid on cell viability in OCT after cold preservation for two weeks in UW solution. Data are presented as the mean ± SE (n = 10). aSignificant difference between the UW and UW with HA groups (P < 0.05). bSignificant difference between the nonpreserved OCT and UW and UW with HA groups (P < 0.05). Fresh: nonpreserved OCT samples.

Mentions: As determined using the WST assay, the supplementation of UW solution with HA800 significantly improved the cell viability of OCTs after 14 days at 4°C compared to nonsupplemented medium (Figure 3). In contrast, UW solution supplemented with either HA1900 or HA6000 did not markedly improve the cell viability of the OCT. These findings indicated that UW solution supplemented with HA800 increased the cell viability of OCTs and reduced the degeneration of chondrocytes during long-term cold storage.


Hyaluronic Acid (800 kDa) Supplementation of University of Wisconsin Solution Improves Viability of Osteochondral Grafts and Reduces Matrix Metalloproteinase Expression during Cold Preservation.

Yamada T, Uchida K, Onuma K, Inoue G, Aikawa J, Takano S, Sekiguchi H, Fujimaki H, Miyagi M, Takaso M - ScientificWorldJournal (2015)

Effect of hyaluronic acid on cell viability in OCT after cold preservation for two weeks in UW solution. Data are presented as the mean ± SE (n = 10). aSignificant difference between the UW and UW with HA groups (P < 0.05). bSignificant difference between the nonpreserved OCT and UW and UW with HA groups (P < 0.05). Fresh: nonpreserved OCT samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496492&req=5

fig3: Effect of hyaluronic acid on cell viability in OCT after cold preservation for two weeks in UW solution. Data are presented as the mean ± SE (n = 10). aSignificant difference between the UW and UW with HA groups (P < 0.05). bSignificant difference between the nonpreserved OCT and UW and UW with HA groups (P < 0.05). Fresh: nonpreserved OCT samples.
Mentions: As determined using the WST assay, the supplementation of UW solution with HA800 significantly improved the cell viability of OCTs after 14 days at 4°C compared to nonsupplemented medium (Figure 3). In contrast, UW solution supplemented with either HA1900 or HA6000 did not markedly improve the cell viability of the OCT. These findings indicated that UW solution supplemented with HA800 increased the cell viability of OCTs and reduced the degeneration of chondrocytes during long-term cold storage.

Bottom Line: The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution.In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT.Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.

ABSTRACT
Osteochondral allografting is a promising option for the treatment of large cartilage defects. However, because the cell viability of osteochondral tissues (OCTs) gradually reduces during storage at 4°C, methods for maintaining the cell viability of fresh OCTs are needed to improve transplantation outcomes. Here, we evaluated whether the supplementation of preservation solution with one of three different molecular weight forms of hyaluronic acid (HA) improved the viability of rat OCTs during long-term cold storage. The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution. In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT. Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA. Although further studies in human OCT are warranted, these findings demonstrate that the use of 800 kDa HA in place of serum may be a suitable approach for the long-term preservation of osteochondral allografts designated for the repair of large cartilage defects in the clinical setting.

No MeSH data available.


Related in: MedlinePlus