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The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression.

He Y, Wang F, Chen S, Liu M, Pan W, Li X - J Diabetes Res (2015)

Bottom Line: Currently there has been no effective treatment of diabetic encephalopathy.Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases.Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Medical University, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, China.

ABSTRACT
Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

No MeSH data available.


Related in: MedlinePlus

Western blot showed the expression of Erk1/2 and pErk1/2 in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
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fig5: Western blot showed the expression of Erk1/2 and pErk1/2 in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.

Mentions: Compared with group A, the phosphorylation level of ERK in the rat hippocampus in group B was elevated (P < 0.05). After Radix Polygoni Multiflori treatment, compared with group B, the phosphorylation level of ERK in the rat hippocampus in group B was significantly reduced. Group C has more significant reduction in ERK phosphorylation in comparison to group D, which also suggests a possible dose-dependent relationship (P < 0.05). Meanwhile, the phosphorylation level of ERK in the rat hippocampal tissue in group C was slightly lower than that in group A (P < 0.01, Figure 5). The phosphorylation level of ERK showed a positive correlation with the expression of MLCK (r = 0.799, P < 0.01, Supplementary Figure 2).


The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression.

He Y, Wang F, Chen S, Liu M, Pan W, Li X - J Diabetes Res (2015)

Western blot showed the expression of Erk1/2 and pErk1/2 in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496489&req=5

fig5: Western blot showed the expression of Erk1/2 and pErk1/2 in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
Mentions: Compared with group A, the phosphorylation level of ERK in the rat hippocampus in group B was elevated (P < 0.05). After Radix Polygoni Multiflori treatment, compared with group B, the phosphorylation level of ERK in the rat hippocampus in group B was significantly reduced. Group C has more significant reduction in ERK phosphorylation in comparison to group D, which also suggests a possible dose-dependent relationship (P < 0.05). Meanwhile, the phosphorylation level of ERK in the rat hippocampal tissue in group C was slightly lower than that in group A (P < 0.01, Figure 5). The phosphorylation level of ERK showed a positive correlation with the expression of MLCK (r = 0.799, P < 0.01, Supplementary Figure 2).

Bottom Line: Currently there has been no effective treatment of diabetic encephalopathy.Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases.Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Medical University, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, China.

ABSTRACT
Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

No MeSH data available.


Related in: MedlinePlus