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The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression.

He Y, Wang F, Chen S, Liu M, Pan W, Li X - J Diabetes Res (2015)

Bottom Line: Currently there has been no effective treatment of diabetic encephalopathy.The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner.Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Medical University, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, China.

ABSTRACT
Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

No MeSH data available.


Related in: MedlinePlus

Western blot showed the expression of MLCK in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
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fig4: Western blot showed the expression of MLCK in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.

Mentions: Western blot showed that, compared with group A, the expression of MLCK in the hippocampal tissue of rats in group B was significantly elevated (P < 0.01). After Radix Polygoni Multiflori treatment, compared with group B, the expression of MLCK in rat hippocampus was significantly reduced. The decrease in MLCK expression in group C was more significant (P < 0.01) than that in group D, suggesting a dose-dependent relationship. Meanwhile, the expression of MLCK in the rat hippocampus in group C was slightly lower than that in group A (P < 0.05, Figure 4).


The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression.

He Y, Wang F, Chen S, Liu M, Pan W, Li X - J Diabetes Res (2015)

Western blot showed the expression of MLCK in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496489&req=5

fig4: Western blot showed the expression of MLCK in hippocampal tissue in rats. (a) The representative photos of western blot. (b) The statistical analysis of western blot. Data shown are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
Mentions: Western blot showed that, compared with group A, the expression of MLCK in the hippocampal tissue of rats in group B was significantly elevated (P < 0.01). After Radix Polygoni Multiflori treatment, compared with group B, the expression of MLCK in rat hippocampus was significantly reduced. The decrease in MLCK expression in group C was more significant (P < 0.01) than that in group D, suggesting a dose-dependent relationship. Meanwhile, the expression of MLCK in the rat hippocampus in group C was slightly lower than that in group A (P < 0.05, Figure 4).

Bottom Line: Currently there has been no effective treatment of diabetic encephalopathy.The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner.Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Medical University, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, China.

ABSTRACT
Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

No MeSH data available.


Related in: MedlinePlus