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The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression.

He Y, Wang F, Chen S, Liu M, Pan W, Li X - J Diabetes Res (2015)

Bottom Line: Currently there has been no effective treatment of diabetic encephalopathy.Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases.Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Medical University, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, China.

ABSTRACT
Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

No MeSH data available.


Related in: MedlinePlus

The ultrastructure of neuronal cells (A1, B1, C1, and D1) and synapses (A2, B2, C2, and D2) of hippocampal tissue in rats of each group. A: control rat; B: diabetic rat; C: diabetic plus high dose RPM; D: diabetic plus low dose RPM. Control rats showed regular nuclear morphology (Figure 3(A1)), large number of synapses in complete form, and rich synaptic vesicles (Figure 3(A2)). Nuclear membrane shrinkage was observed in diabetic rats and cell membrane structure was also unclear (Figure 3(B1)). Figure 3(B2) showed significantly reduced number of synapses, abnormal synaptic structure, and blur or missing synaptic cleft. Rat neuronal cell structure in diabetic plus RPM had been greatly improved compared with diabetic rats, in which diabetic plus high dose RPM was significantly higher than of in low dose RPM (Figures 3(C1), 3(D1)3(C2), and 3(D2)).
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fig3: The ultrastructure of neuronal cells (A1, B1, C1, and D1) and synapses (A2, B2, C2, and D2) of hippocampal tissue in rats of each group. A: control rat; B: diabetic rat; C: diabetic plus high dose RPM; D: diabetic plus low dose RPM. Control rats showed regular nuclear morphology (Figure 3(A1)), large number of synapses in complete form, and rich synaptic vesicles (Figure 3(A2)). Nuclear membrane shrinkage was observed in diabetic rats and cell membrane structure was also unclear (Figure 3(B1)). Figure 3(B2) showed significantly reduced number of synapses, abnormal synaptic structure, and blur or missing synaptic cleft. Rat neuronal cell structure in diabetic plus RPM had been greatly improved compared with diabetic rats, in which diabetic plus high dose RPM was significantly higher than of in low dose RPM (Figures 3(C1), 3(D1)3(C2), and 3(D2)).

Mentions: Transmission electronic microscopy found that the hippocampal neurons of rats in group A showed regular nuclear morphology, where nuclear chromatin was rich and evenly distributed with prominent nucleoli and organelles were abundant in the cytoplasm including evenly distributed endoplasmic reticulum and tightly packed mitochondria (Figure 3(A1)). Large number of synapses in complete form and abundant synaptic vesicles were observed (Figure 3(A2)). The hippocampal neurons of rats in group B showed nuclear membrane shrinkage, with unevenly distributed nuclear chromatin, unclear cell membrane, irregularly distributed endoplasmic reticulum, and swollen mitochondria in irregular shape (Figure 3(B1)). In addition, the numbers of synapses and synaptic vesicles were significantly reduced, synaptic structure was interrupted, postsynaptic membrane was swelling, and synaptic cleft was blurred or even disappeared (Figure 3(B2)).


The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression.

He Y, Wang F, Chen S, Liu M, Pan W, Li X - J Diabetes Res (2015)

The ultrastructure of neuronal cells (A1, B1, C1, and D1) and synapses (A2, B2, C2, and D2) of hippocampal tissue in rats of each group. A: control rat; B: diabetic rat; C: diabetic plus high dose RPM; D: diabetic plus low dose RPM. Control rats showed regular nuclear morphology (Figure 3(A1)), large number of synapses in complete form, and rich synaptic vesicles (Figure 3(A2)). Nuclear membrane shrinkage was observed in diabetic rats and cell membrane structure was also unclear (Figure 3(B1)). Figure 3(B2) showed significantly reduced number of synapses, abnormal synaptic structure, and blur or missing synaptic cleft. Rat neuronal cell structure in diabetic plus RPM had been greatly improved compared with diabetic rats, in which diabetic plus high dose RPM was significantly higher than of in low dose RPM (Figures 3(C1), 3(D1)3(C2), and 3(D2)).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4496489&req=5

fig3: The ultrastructure of neuronal cells (A1, B1, C1, and D1) and synapses (A2, B2, C2, and D2) of hippocampal tissue in rats of each group. A: control rat; B: diabetic rat; C: diabetic plus high dose RPM; D: diabetic plus low dose RPM. Control rats showed regular nuclear morphology (Figure 3(A1)), large number of synapses in complete form, and rich synaptic vesicles (Figure 3(A2)). Nuclear membrane shrinkage was observed in diabetic rats and cell membrane structure was also unclear (Figure 3(B1)). Figure 3(B2) showed significantly reduced number of synapses, abnormal synaptic structure, and blur or missing synaptic cleft. Rat neuronal cell structure in diabetic plus RPM had been greatly improved compared with diabetic rats, in which diabetic plus high dose RPM was significantly higher than of in low dose RPM (Figures 3(C1), 3(D1)3(C2), and 3(D2)).
Mentions: Transmission electronic microscopy found that the hippocampal neurons of rats in group A showed regular nuclear morphology, where nuclear chromatin was rich and evenly distributed with prominent nucleoli and organelles were abundant in the cytoplasm including evenly distributed endoplasmic reticulum and tightly packed mitochondria (Figure 3(A1)). Large number of synapses in complete form and abundant synaptic vesicles were observed (Figure 3(A2)). The hippocampal neurons of rats in group B showed nuclear membrane shrinkage, with unevenly distributed nuclear chromatin, unclear cell membrane, irregularly distributed endoplasmic reticulum, and swollen mitochondria in irregular shape (Figure 3(B1)). In addition, the numbers of synapses and synaptic vesicles were significantly reduced, synaptic structure was interrupted, postsynaptic membrane was swelling, and synaptic cleft was blurred or even disappeared (Figure 3(B2)).

Bottom Line: Currently there has been no effective treatment of diabetic encephalopathy.Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases.Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

View Article: PubMed Central - PubMed

Affiliation: Guizhou Medical University, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550025, China.

ABSTRACT
Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer's diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P < 0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

No MeSH data available.


Related in: MedlinePlus