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Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1α Pathway.

Zhang Y, He Y, Yu H, Ma F, Wu J, Zhang X - Evid Based Complement Alternat Med (2015)

Bottom Line: The lack of effective treatment for liver cirrhosis and hepatocellular carcinomas imposes serious challenges to the healthcare system.The results demonstrated that liquiritigenin is effective in protecting liver from injury or treating chronic liver diseases.The modulation of PGC-1α and its downstream genes might play a critical role in relieving CCl4-induced hepatic pathogenesis by liquiritigenin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China ; School of Basic Medical Science, Jiujiang University, Jiujiang, Jianxi 332000, China.

ABSTRACT
The lack of effective treatment for liver cirrhosis and hepatocellular carcinomas imposes serious challenges to the healthcare system. Here, we investigated the efficacy and mechanism of liquiritigenin involved in preventing or retarding the progression of liver diseases in a rat model with chronic carbon tetrachloride (CCl4) exposure. Sprague Dawley rats were given CCl4 and lliquiritigenin alone or simultaneously for 8 weeks before liver was harvested to check histological changes by Hematoxylin and Eosin (H&E) staining, apoptosis by TUNEL assay, ROS by dihydroethidium staining, antioxidant enzyme activities and malondialdehyde using specific kits, and gene expression by quantitative real-time PCR and western blot. Chronic CCl4 exposure caused profound changes in liver histology with extensive hepatocyte death (necrosis and apoptosis), fat accumulation, and infiltration of inflammatory cells, accompanied by depressed activities of antioxidant enzymes, increased oxidative stress, elevated expression of inflammation and fibrotic genes, and downregulation of PGC-1α, ND1, and Bcl-x in rat liver. All these changes were abolished or alleviated by lliquiritigenin. The results demonstrated that liquiritigenin is effective in protecting liver from injury or treating chronic liver diseases. The modulation of PGC-1α and its downstream genes might play a critical role in relieving CCl4-induced hepatic pathogenesis by liquiritigenin.

No MeSH data available.


Related in: MedlinePlus

Liquiritigenin overturned the abnormal gene expression caused by CCl4. The expression of examined genes was analyzed by quantitative real-time PCR ((a) and (b)) and western blot (c). ∗P < 0.05 compared to control rats; ∧P < 0.05 compared to CCl4 treated rats.
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fig5: Liquiritigenin overturned the abnormal gene expression caused by CCl4. The expression of examined genes was analyzed by quantitative real-time PCR ((a) and (b)) and western blot (c). ∗P < 0.05 compared to control rats; ∧P < 0.05 compared to CCl4 treated rats.

Mentions: The mRNA levels of RelA and MMP2 were increased by more than 5- and 7.5-fold, respectively, over control rats by CCl4 treatment, which was reduced by about 66% and 58% by liquiritigenin (Figure 5(a)). Consequently, the protein levels of RelA and MMP2 were significantly increased in livers of CCl4 treated rats and the increase was attenuated by liquiritigenin (Figure 5(c)).


Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1α Pathway.

Zhang Y, He Y, Yu H, Ma F, Wu J, Zhang X - Evid Based Complement Alternat Med (2015)

Liquiritigenin overturned the abnormal gene expression caused by CCl4. The expression of examined genes was analyzed by quantitative real-time PCR ((a) and (b)) and western blot (c). ∗P < 0.05 compared to control rats; ∧P < 0.05 compared to CCl4 treated rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496487&req=5

fig5: Liquiritigenin overturned the abnormal gene expression caused by CCl4. The expression of examined genes was analyzed by quantitative real-time PCR ((a) and (b)) and western blot (c). ∗P < 0.05 compared to control rats; ∧P < 0.05 compared to CCl4 treated rats.
Mentions: The mRNA levels of RelA and MMP2 were increased by more than 5- and 7.5-fold, respectively, over control rats by CCl4 treatment, which was reduced by about 66% and 58% by liquiritigenin (Figure 5(a)). Consequently, the protein levels of RelA and MMP2 were significantly increased in livers of CCl4 treated rats and the increase was attenuated by liquiritigenin (Figure 5(c)).

Bottom Line: The lack of effective treatment for liver cirrhosis and hepatocellular carcinomas imposes serious challenges to the healthcare system.The results demonstrated that liquiritigenin is effective in protecting liver from injury or treating chronic liver diseases.The modulation of PGC-1α and its downstream genes might play a critical role in relieving CCl4-induced hepatic pathogenesis by liquiritigenin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China ; School of Basic Medical Science, Jiujiang University, Jiujiang, Jianxi 332000, China.

ABSTRACT
The lack of effective treatment for liver cirrhosis and hepatocellular carcinomas imposes serious challenges to the healthcare system. Here, we investigated the efficacy and mechanism of liquiritigenin involved in preventing or retarding the progression of liver diseases in a rat model with chronic carbon tetrachloride (CCl4) exposure. Sprague Dawley rats were given CCl4 and lliquiritigenin alone or simultaneously for 8 weeks before liver was harvested to check histological changes by Hematoxylin and Eosin (H&E) staining, apoptosis by TUNEL assay, ROS by dihydroethidium staining, antioxidant enzyme activities and malondialdehyde using specific kits, and gene expression by quantitative real-time PCR and western blot. Chronic CCl4 exposure caused profound changes in liver histology with extensive hepatocyte death (necrosis and apoptosis), fat accumulation, and infiltration of inflammatory cells, accompanied by depressed activities of antioxidant enzymes, increased oxidative stress, elevated expression of inflammation and fibrotic genes, and downregulation of PGC-1α, ND1, and Bcl-x in rat liver. All these changes were abolished or alleviated by lliquiritigenin. The results demonstrated that liquiritigenin is effective in protecting liver from injury or treating chronic liver diseases. The modulation of PGC-1α and its downstream genes might play a critical role in relieving CCl4-induced hepatic pathogenesis by liquiritigenin.

No MeSH data available.


Related in: MedlinePlus