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Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice.

Lee YS, Park MJ, Park SH, Koo JS, Moon HS, Joo BS - Clin Exp Reprod Med (2015)

Bottom Line: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05).These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Cardiology, Dong-A University Hospital, Busan, Korea.

ABSTRACT

Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.

Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm.

Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.

Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

No MeSH data available.


Related in: MedlinePlus

In young mice (6-11 weeks), the representative photographs for the formation and early hatching of blastocysts 24 hours after laser-assisted zona thinning (LAZT) in the morula-stage embryos. (A) Control, (B) LAZT1, (C) LAZT4. ×100.
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Figure 2: In young mice (6-11 weeks), the representative photographs for the formation and early hatching of blastocysts 24 hours after laser-assisted zona thinning (LAZT) in the morula-stage embryos. (A) Control, (B) LAZT1, (C) LAZT4. ×100.

Mentions: LAZT was performed in morula-stage embryos of young mice as in the 8-cell-stage embryos. The blastocyst formation rates in the control, LAZT1, and LAZT4 groups were 67.3%, 77.8%, and 83.3%, respectively, with no significant differences among the three groups. However, the hatching rates 120 hours after zygote collection were significantly higher in the LAZT1 (61.1%) and LAZT4 (77.8%) groups than in the control group (17.3%) (p<0.001). The early hatching rate was also significantly higher in both LAZT groups than in the control group (p<0.001) (Table 2). Figure 2 shows the representative photographs for blastocyst formation and early hatching 24 hours after LAZT in the morula-stage embryos.


Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice.

Lee YS, Park MJ, Park SH, Koo JS, Moon HS, Joo BS - Clin Exp Reprod Med (2015)

In young mice (6-11 weeks), the representative photographs for the formation and early hatching of blastocysts 24 hours after laser-assisted zona thinning (LAZT) in the morula-stage embryos. (A) Control, (B) LAZT1, (C) LAZT4. ×100.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496431&req=5

Figure 2: In young mice (6-11 weeks), the representative photographs for the formation and early hatching of blastocysts 24 hours after laser-assisted zona thinning (LAZT) in the morula-stage embryos. (A) Control, (B) LAZT1, (C) LAZT4. ×100.
Mentions: LAZT was performed in morula-stage embryos of young mice as in the 8-cell-stage embryos. The blastocyst formation rates in the control, LAZT1, and LAZT4 groups were 67.3%, 77.8%, and 83.3%, respectively, with no significant differences among the three groups. However, the hatching rates 120 hours after zygote collection were significantly higher in the LAZT1 (61.1%) and LAZT4 (77.8%) groups than in the control group (17.3%) (p<0.001). The early hatching rate was also significantly higher in both LAZT groups than in the control group (p<0.001) (Table 2). Figure 2 shows the representative photographs for blastocyst formation and early hatching 24 hours after LAZT in the morula-stage embryos.

Bottom Line: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05).These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Cardiology, Dong-A University Hospital, Busan, Korea.

ABSTRACT

Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.

Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm.

Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.

Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

No MeSH data available.


Related in: MedlinePlus