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Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice.

Lee YS, Park MJ, Park SH, Koo JS, Moon HS, Joo BS - Clin Exp Reprod Med (2015)

Bottom Line: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05).These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Cardiology, Dong-A University Hospital, Busan, Korea.

ABSTRACT

Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.

Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm.

Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.

Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

No MeSH data available.


Related in: MedlinePlus

Laser-assisted zona thinning (LAZT) in the 8-cell-stage (A, C) and the morula-stage embryos (B, D). (A, B) One-point zona thinning (LAZT1). (C, D) Four-point zona thinning (LAZT4). ×400.
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Figure 1: Laser-assisted zona thinning (LAZT) in the 8-cell-stage (A, C) and the morula-stage embryos (B, D). (A, B) One-point zona thinning (LAZT1). (C, D) Four-point zona thinning (LAZT4). ×400.

Mentions: LAH was performed by ZT in 8-cell-stage or morula-stage embryos by using a noncontact 1.5-µm Zilos-tk Laser system (Hamilton Thorne, Beverly, MA, USA). The original culture dish containing the embryos (30-µL drops of the medium) was removed from the incubator and placed on an inverted microscope equipped with a laser system. The point of treatment on the ZP was carefully focused upon and treated with the laser. At this time, the embryos were not stabilized with the holding pipette. One point of treatment on the ZP was randomly chosen, and the sites of four points were determined at 90° intervals from the randomly chosen point. The ZP was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm on the ZP at one point (LAZT1, Figure 1A, B) or four points (LAZT4, Figure 1C, D). All ZT was performed in the empty region without contact to the blastomere in order to minimize the blastomere damage. The laser power was 100%, and the pulse duration was 500 µs. In the control group, embryos were replaced without LAH.


Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice.

Lee YS, Park MJ, Park SH, Koo JS, Moon HS, Joo BS - Clin Exp Reprod Med (2015)

Laser-assisted zona thinning (LAZT) in the 8-cell-stage (A, C) and the morula-stage embryos (B, D). (A, B) One-point zona thinning (LAZT1). (C, D) Four-point zona thinning (LAZT4). ×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496431&req=5

Figure 1: Laser-assisted zona thinning (LAZT) in the 8-cell-stage (A, C) and the morula-stage embryos (B, D). (A, B) One-point zona thinning (LAZT1). (C, D) Four-point zona thinning (LAZT4). ×400.
Mentions: LAH was performed by ZT in 8-cell-stage or morula-stage embryos by using a noncontact 1.5-µm Zilos-tk Laser system (Hamilton Thorne, Beverly, MA, USA). The original culture dish containing the embryos (30-µL drops of the medium) was removed from the incubator and placed on an inverted microscope equipped with a laser system. The point of treatment on the ZP was carefully focused upon and treated with the laser. At this time, the embryos were not stabilized with the holding pipette. One point of treatment on the ZP was randomly chosen, and the sites of four points were determined at 90° intervals from the randomly chosen point. The ZP was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm on the ZP at one point (LAZT1, Figure 1A, B) or four points (LAZT4, Figure 1C, D). All ZT was performed in the empty region without contact to the blastomere in order to minimize the blastomere damage. The laser power was 100%, and the pulse duration was 500 µs. In the control group, embryos were replaced without LAH.

Bottom Line: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05).These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Cardiology, Dong-A University Hospital, Busan, Korea.

ABSTRACT

Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.

Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm.

Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.

Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

No MeSH data available.


Related in: MedlinePlus