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Circadian genes Per1 and Per2 increase radiosensitivity of glioma in vivo.

Zhanfeng N, Yanhui L, Zhou F, Shaocai H, Guangxing L, Hechun X - Oncotarget (2015)

Bottom Line: Per1 and Per2 play a key role in regulating the circadian rhythm in mammals.No such sensitizing effect was observed in normal tissue.Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, The General Hospital of Ningxia Medical University, Yinchuan, China.

ABSTRACT
Per1 and Per2 play a key role in regulating the circadian rhythm in mammals. We report here that although both genes were expressed with a circadian rhythm in glioma and normal brain tissue in rats, their expression profiles differed in the two types of tissue. In addition, high expression of Per1 and Per2 in glioma tissue was associated with increased sensitivity to x-irradiation. No such sensitizing effect was observed in normal tissue. Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

No MeSH data available.


Related in: MedlinePlus

TUNEL assay to detect apoptosis in glioma and normal tissue at times of high and low expression of Per1 following a single dose of x-radiation (15 Gy)Bar, 50 μm.
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Figure 3: TUNEL assay to detect apoptosis in glioma and normal tissue at times of high and low expression of Per1 following a single dose of x-radiation (15 Gy)Bar, 50 μm.

Mentions: We detected apoptosis in glioma and normal tissue after irradiating animals at times when Per1 and Per2 mRNA levels were high and low. In glioma tissue, Per1 expression showed a period of approximately 12 h (Figure 3A, 3B). The proportion of apoptotic cells at ZT4 (28.1%, p > 0.05), when Per1 expression was at its peak, was not significantly different than at ZT12 (31.7%, p > 0.05), when Per1 expression was at a nadir. Similarly, in normal tissue (Figure 3C, 3D), the proportion of apoptotic cells was not significantly different between ZT4 (7.0%, p > 0.05) and ZT12 (6.1%, p > 0.05).


Circadian genes Per1 and Per2 increase radiosensitivity of glioma in vivo.

Zhanfeng N, Yanhui L, Zhou F, Shaocai H, Guangxing L, Hechun X - Oncotarget (2015)

TUNEL assay to detect apoptosis in glioma and normal tissue at times of high and low expression of Per1 following a single dose of x-radiation (15 Gy)Bar, 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496409&req=5

Figure 3: TUNEL assay to detect apoptosis in glioma and normal tissue at times of high and low expression of Per1 following a single dose of x-radiation (15 Gy)Bar, 50 μm.
Mentions: We detected apoptosis in glioma and normal tissue after irradiating animals at times when Per1 and Per2 mRNA levels were high and low. In glioma tissue, Per1 expression showed a period of approximately 12 h (Figure 3A, 3B). The proportion of apoptotic cells at ZT4 (28.1%, p > 0.05), when Per1 expression was at its peak, was not significantly different than at ZT12 (31.7%, p > 0.05), when Per1 expression was at a nadir. Similarly, in normal tissue (Figure 3C, 3D), the proportion of apoptotic cells was not significantly different between ZT4 (7.0%, p > 0.05) and ZT12 (6.1%, p > 0.05).

Bottom Line: Per1 and Per2 play a key role in regulating the circadian rhythm in mammals.No such sensitizing effect was observed in normal tissue.Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, The General Hospital of Ningxia Medical University, Yinchuan, China.

ABSTRACT
Per1 and Per2 play a key role in regulating the circadian rhythm in mammals. We report here that although both genes were expressed with a circadian rhythm in glioma and normal brain tissue in rats, their expression profiles differed in the two types of tissue. In addition, high expression of Per1 and Per2 in glioma tissue was associated with increased sensitivity to x-irradiation. No such sensitizing effect was observed in normal tissue. Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

No MeSH data available.


Related in: MedlinePlus