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Circadian genes Per1 and Per2 increase radiosensitivity of glioma in vivo.

Zhanfeng N, Yanhui L, Zhou F, Shaocai H, Guangxing L, Hechun X - Oncotarget (2015)

Bottom Line: Per1 and Per2 play a key role in regulating the circadian rhythm in mammals.No such sensitizing effect was observed in normal tissue.Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, The General Hospital of Ningxia Medical University, Yinchuan, China.

ABSTRACT
Per1 and Per2 play a key role in regulating the circadian rhythm in mammals. We report here that although both genes were expressed with a circadian rhythm in glioma and normal brain tissue in rats, their expression profiles differed in the two types of tissue. In addition, high expression of Per1 and Per2 in glioma tissue was associated with increased sensitivity to x-irradiation. No such sensitizing effect was observed in normal tissue. Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

No MeSH data available.


Related in: MedlinePlus

Changes in Per1 and Per2 mRNA expression in glioma and normal tissues after ionizing irradiationGlioma and normal tissues were treated with ionizing radiation (IR) or not (untreated, UT), then total RNA was extracted and used as template in real-time RT-PCR to measure Per1 and Per2 mRNA expression. The relative level of each mRNA was normalized to the corresponding level of β-actin mRNA. Data are the mean and standard deviation of three independent experiments.
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Figure 1: Changes in Per1 and Per2 mRNA expression in glioma and normal tissues after ionizing irradiationGlioma and normal tissues were treated with ionizing radiation (IR) or not (untreated, UT), then total RNA was extracted and used as template in real-time RT-PCR to measure Per1 and Per2 mRNA expression. The relative level of each mRNA was normalized to the corresponding level of β-actin mRNA. Data are the mean and standard deviation of three independent experiments.

Mentions: We used real-time RT-PCR to measure Per1 and Per2 mRNA levels in glioma and normal tissues after treatment with ionizing radiation (15 Gy). In normal tissue, we compared Per1 mRNA levels at ZT4, when Per1 expression was high in glioma tissue. The levels did not differ significantly between the irradiated and untreated (control) groups at this time (t = –1.405, p > 0.05). However, when we compared the levels at ZT12, when Per1 expression was low in glioma, the level in the irradiated group was significantly higher than in the control group (t = –5.135, p < 0.001, Figure 1). Per2 mRNA levels did not differ significantly between the irradiated and untreated (control) groups at either ZT0 (t = –1.742, p > 0.1) or ZT8 (t = –0.642, p > 0.5) (Figure 1), when Per2 expression in glioma tissue was high or low, respectively. In glioma tissue, the levels of Per1 and Per2 mRNA were significantly higher in the irradiated group than in the control group when Per1 and Per2 expression was elevated, i.e. at ZT4 for Per1 (t = –4.464, p < 0.001) and ZT0 for Per2 (t = –2.407, p < 0.05). Similar results were observed when Per1 and Per2 expression was low, i.e. at ZT12 for Per1 (t = –5.276, p < 0.001) and ZT8 for Per2 (t = –6.599, p < 0.001) (Figure 1).


Circadian genes Per1 and Per2 increase radiosensitivity of glioma in vivo.

Zhanfeng N, Yanhui L, Zhou F, Shaocai H, Guangxing L, Hechun X - Oncotarget (2015)

Changes in Per1 and Per2 mRNA expression in glioma and normal tissues after ionizing irradiationGlioma and normal tissues were treated with ionizing radiation (IR) or not (untreated, UT), then total RNA was extracted and used as template in real-time RT-PCR to measure Per1 and Per2 mRNA expression. The relative level of each mRNA was normalized to the corresponding level of β-actin mRNA. Data are the mean and standard deviation of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496409&req=5

Figure 1: Changes in Per1 and Per2 mRNA expression in glioma and normal tissues after ionizing irradiationGlioma and normal tissues were treated with ionizing radiation (IR) or not (untreated, UT), then total RNA was extracted and used as template in real-time RT-PCR to measure Per1 and Per2 mRNA expression. The relative level of each mRNA was normalized to the corresponding level of β-actin mRNA. Data are the mean and standard deviation of three independent experiments.
Mentions: We used real-time RT-PCR to measure Per1 and Per2 mRNA levels in glioma and normal tissues after treatment with ionizing radiation (15 Gy). In normal tissue, we compared Per1 mRNA levels at ZT4, when Per1 expression was high in glioma tissue. The levels did not differ significantly between the irradiated and untreated (control) groups at this time (t = –1.405, p > 0.05). However, when we compared the levels at ZT12, when Per1 expression was low in glioma, the level in the irradiated group was significantly higher than in the control group (t = –5.135, p < 0.001, Figure 1). Per2 mRNA levels did not differ significantly between the irradiated and untreated (control) groups at either ZT0 (t = –1.742, p > 0.1) or ZT8 (t = –0.642, p > 0.5) (Figure 1), when Per2 expression in glioma tissue was high or low, respectively. In glioma tissue, the levels of Per1 and Per2 mRNA were significantly higher in the irradiated group than in the control group when Per1 and Per2 expression was elevated, i.e. at ZT4 for Per1 (t = –4.464, p < 0.001) and ZT0 for Per2 (t = –2.407, p < 0.05). Similar results were observed when Per1 and Per2 expression was low, i.e. at ZT12 for Per1 (t = –5.276, p < 0.001) and ZT8 for Per2 (t = –6.599, p < 0.001) (Figure 1).

Bottom Line: Per1 and Per2 play a key role in regulating the circadian rhythm in mammals.No such sensitizing effect was observed in normal tissue.Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, The General Hospital of Ningxia Medical University, Yinchuan, China.

ABSTRACT
Per1 and Per2 play a key role in regulating the circadian rhythm in mammals. We report here that although both genes were expressed with a circadian rhythm in glioma and normal brain tissue in rats, their expression profiles differed in the two types of tissue. In addition, high expression of Per1 and Per2 in glioma tissue was associated with increased sensitivity to x-irradiation. No such sensitizing effect was observed in normal tissue. Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.

No MeSH data available.


Related in: MedlinePlus