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Deregulation of dicer and mir-155 expression in liposarcoma.

Vincenzi B, Iuliani M, Zoccoli A, Pantano F, Fioramonti M, De Lisi D, Frezza AM, Rabitti C, Perrone G, Onetti Muda A, Russo A, Giordano A, Santini D, Dei Tos AP, Tonini G - Oncotarget (2015)

Bottom Line: Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors.The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ.Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.

ABSTRACT
Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis.

No MeSH data available.


Related in: MedlinePlus

In silico analyses(A) Dicer expression in three different Case Sets: MLPS/RLPS (21 samples), Dicer altered in 9 (43%); DDLPS (50 samples), Dicer altered in 19 (38%); PLPS (24 samples), Dicer altered in 15 (63%). (B) Dicer mRNA levels in different LPS histologies compared to normal fat. (p value > 0.05).
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Figure 1: In silico analyses(A) Dicer expression in three different Case Sets: MLPS/RLPS (21 samples), Dicer altered in 9 (43%); DDLPS (50 samples), Dicer altered in 19 (38%); PLPS (24 samples), Dicer altered in 15 (63%). (B) Dicer mRNA levels in different LPS histologies compared to normal fat. (p value > 0.05).

Mentions: In order to get preliminary information about Dicer expression in LPS, we analyzed the transcriptomic profiles of 21 MLPS/RLPS, 50 DDLPS, 24 PLPS and 9 normal fat (GSE 21124) searching for Dicer expression level and we found no significant changes in mRNA levels across the different histologies (Figure 1A and Figure 1B). Starting from these results we decided to perform a second analysis in our series using a more reliable and sensitive method such as qRT-PCR.


Deregulation of dicer and mir-155 expression in liposarcoma.

Vincenzi B, Iuliani M, Zoccoli A, Pantano F, Fioramonti M, De Lisi D, Frezza AM, Rabitti C, Perrone G, Onetti Muda A, Russo A, Giordano A, Santini D, Dei Tos AP, Tonini G - Oncotarget (2015)

In silico analyses(A) Dicer expression in three different Case Sets: MLPS/RLPS (21 samples), Dicer altered in 9 (43%); DDLPS (50 samples), Dicer altered in 19 (38%); PLPS (24 samples), Dicer altered in 15 (63%). (B) Dicer mRNA levels in different LPS histologies compared to normal fat. (p value > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496377&req=5

Figure 1: In silico analyses(A) Dicer expression in three different Case Sets: MLPS/RLPS (21 samples), Dicer altered in 9 (43%); DDLPS (50 samples), Dicer altered in 19 (38%); PLPS (24 samples), Dicer altered in 15 (63%). (B) Dicer mRNA levels in different LPS histologies compared to normal fat. (p value > 0.05).
Mentions: In order to get preliminary information about Dicer expression in LPS, we analyzed the transcriptomic profiles of 21 MLPS/RLPS, 50 DDLPS, 24 PLPS and 9 normal fat (GSE 21124) searching for Dicer expression level and we found no significant changes in mRNA levels across the different histologies (Figure 1A and Figure 1B). Starting from these results we decided to perform a second analysis in our series using a more reliable and sensitive method such as qRT-PCR.

Bottom Line: Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors.The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ.Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy.

ABSTRACT
Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis.

No MeSH data available.


Related in: MedlinePlus