Limits...
Vasorin is a potential serum biomarker and drug target of hepatocarcinoma screened by subtractive-EMSA-SELEX to clinic patient serum.

Li S, Li H, Yang X, Wang W, Huang A, Li J, Qin X, Li F, Lu G, Ding H, Su X, Hou L, Xia W, Shi M, Zhang H, Zhao Q, Dong J, Ge X, Sun L, Bai C, Wang C, Shen X, Fang T, Wang F, Zhang H, Shao N - Oncotarget (2015)

Bottom Line: VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients.As a membrane protein and/or as free protein, VASN may be an effective target for biological treatment of liver cancer and is a potential biomarker for HCC diagnosis.Small molecular nucleotides targeting VASN are promising biological therapies to HCC.

View Article: PubMed Central - PubMed

Affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.

ABSTRACT

Unlabelled: We report a new biomarker of hepatocarcinoma, vasorin (VASN), screened by a subtractive EMSA-SELEX strategy from AFP negative serum of hepatocellular carcinoma (HCC) patients with extrahepatic metastases. VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients. Further validation by Q-PCR,IFA and Western blot showed higher expression of VASN at mRNA and protein levels in HCC cell lines and HCC tissues than in normal controls. RNA interference and forced overexpression assays verified that VASN promotes cell proliferation and migration and inhibits apoptosis. Down-regulation of microRNA miR145 and miR146a is an important mechanism leading to high expression of VASN.

Conclusion: As a membrane protein and/or as free protein, VASN may be an effective target for biological treatment of liver cancer and is a potential biomarker for HCC diagnosis. Small molecular nucleotides targeting VASN are promising biological therapies to HCC.

No MeSH data available.


Related in: MedlinePlus

VASN is identified as a candidate biomarker from AFP negative serum samples of HCC patients by subtractive-EMSA-SELEX(a) Schematic showing selection of specific aptamers by subtractive-EMSA-SELEX from liquid serum samples. The red box indicates in-gel ssDNAs which should be collected and amplified for the next round selection. (b) The fifth pool demonstrated an enrichment and discrimination for the serum sample used for selection. (c) The fifth pool demonstrated an enrichment and discrimination for different serum samples. The arrows indicate the complex band of aptamer and targets.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4496339&req=5

Figure 1: VASN is identified as a candidate biomarker from AFP negative serum samples of HCC patients by subtractive-EMSA-SELEX(a) Schematic showing selection of specific aptamers by subtractive-EMSA-SELEX from liquid serum samples. The red box indicates in-gel ssDNAs which should be collected and amplified for the next round selection. (b) The fifth pool demonstrated an enrichment and discrimination for the serum sample used for selection. (c) The fifth pool demonstrated an enrichment and discrimination for different serum samples. The arrows indicate the complex band of aptamer and targets.

Mentions: To identify HCC serum biomarker, we developed a subtractive - EMSA-SELEX strategy targeting AFP negative serum samples of HCC patients with extra hepatic metastases (Fig. 1a). After each round of selection, the enriched pool was labeled with FAM (carboxyfluorescein) by a modified 5′ primer through unequal length PCR. Enrichment of each round of selection was monitored by EMSA of the labeled pool and the targets or the counter targets complex. Selection rounds were repeated until there was an obvious binding of enriched pool with targets and until the enriched pool could discriminate targets from counter-targets. In this study, the fifth pool demonstrated an obvious enrichment and discrimination between counter target and target as well as between normal serum and AFP negative HCC serum (Figs. 1b, 1c).


Vasorin is a potential serum biomarker and drug target of hepatocarcinoma screened by subtractive-EMSA-SELEX to clinic patient serum.

Li S, Li H, Yang X, Wang W, Huang A, Li J, Qin X, Li F, Lu G, Ding H, Su X, Hou L, Xia W, Shi M, Zhang H, Zhao Q, Dong J, Ge X, Sun L, Bai C, Wang C, Shen X, Fang T, Wang F, Zhang H, Shao N - Oncotarget (2015)

VASN is identified as a candidate biomarker from AFP negative serum samples of HCC patients by subtractive-EMSA-SELEX(a) Schematic showing selection of specific aptamers by subtractive-EMSA-SELEX from liquid serum samples. The red box indicates in-gel ssDNAs which should be collected and amplified for the next round selection. (b) The fifth pool demonstrated an enrichment and discrimination for the serum sample used for selection. (c) The fifth pool demonstrated an enrichment and discrimination for different serum samples. The arrows indicate the complex band of aptamer and targets.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496339&req=5

Figure 1: VASN is identified as a candidate biomarker from AFP negative serum samples of HCC patients by subtractive-EMSA-SELEX(a) Schematic showing selection of specific aptamers by subtractive-EMSA-SELEX from liquid serum samples. The red box indicates in-gel ssDNAs which should be collected and amplified for the next round selection. (b) The fifth pool demonstrated an enrichment and discrimination for the serum sample used for selection. (c) The fifth pool demonstrated an enrichment and discrimination for different serum samples. The arrows indicate the complex band of aptamer and targets.
Mentions: To identify HCC serum biomarker, we developed a subtractive - EMSA-SELEX strategy targeting AFP negative serum samples of HCC patients with extra hepatic metastases (Fig. 1a). After each round of selection, the enriched pool was labeled with FAM (carboxyfluorescein) by a modified 5′ primer through unequal length PCR. Enrichment of each round of selection was monitored by EMSA of the labeled pool and the targets or the counter targets complex. Selection rounds were repeated until there was an obvious binding of enriched pool with targets and until the enriched pool could discriminate targets from counter-targets. In this study, the fifth pool demonstrated an obvious enrichment and discrimination between counter target and target as well as between normal serum and AFP negative HCC serum (Figs. 1b, 1c).

Bottom Line: VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients.As a membrane protein and/or as free protein, VASN may be an effective target for biological treatment of liver cancer and is a potential biomarker for HCC diagnosis.Small molecular nucleotides targeting VASN are promising biological therapies to HCC.

View Article: PubMed Central - PubMed

Affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.

ABSTRACT

Unlabelled: We report a new biomarker of hepatocarcinoma, vasorin (VASN), screened by a subtractive EMSA-SELEX strategy from AFP negative serum of hepatocellular carcinoma (HCC) patients with extrahepatic metastases. VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients. Further validation by Q-PCR,IFA and Western blot showed higher expression of VASN at mRNA and protein levels in HCC cell lines and HCC tissues than in normal controls. RNA interference and forced overexpression assays verified that VASN promotes cell proliferation and migration and inhibits apoptosis. Down-regulation of microRNA miR145 and miR146a is an important mechanism leading to high expression of VASN.

Conclusion: As a membrane protein and/or as free protein, VASN may be an effective target for biological treatment of liver cancer and is a potential biomarker for HCC diagnosis. Small molecular nucleotides targeting VASN are promising biological therapies to HCC.

No MeSH data available.


Related in: MedlinePlus