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miR-211 suppresses hepatocellular carcinoma by downregulating SATB2.

Jiang G, Cui Y, Yu X, Wu Z, Ding G, Cao L - Oncotarget (2015)

Bottom Line: Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues.We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells.This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatopancreatobiliary Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

ABSTRACT
Dysregulation of microRNAs (miRs) is involved in carcinogenesis. Deregulation of miR-211 has recently been observed in many tumors, but its function in hepatocellular carcinoma (HCC) is still unknown. Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues. We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells. Luciferase reporter assays and western blot indicated that special AT-rich sequence-binding protein-2 (SATB2), is a direct target of miR-211. The expression of SATB2 was upregulated in HCC cancer tissues and cell lines and miR-211 levels inversely correlated with SATB2 levels in HCC. Importantly, SATB2 rescued the miR-211-mediated inhibition of cell invasion and proliferation. Finally, reintroduction of miR-211 repressed tumor formation of HCC in xenograft mice. This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

No MeSH data available.


Related in: MedlinePlus

Upregulation of miR-211 inhibits cell invasion(A) Upexpression of miR-211 can significantly promoted the HepG2 cells invasion whereas miR-211 inhibitor inhibitedHepG2cell invasion. The relative invasive cells of each group have been shown in the right. (B) Upexpression of miR-211 can significantly promoted the SMMC7721 cells invasion whereas miR-211 inhibitor inhibited SMMC7721 cell invasion. The relative invasive cells of each group have been shown in the right. ***p < 0.001.
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Figure 3: Upregulation of miR-211 inhibits cell invasion(A) Upexpression of miR-211 can significantly promoted the HepG2 cells invasion whereas miR-211 inhibitor inhibitedHepG2cell invasion. The relative invasive cells of each group have been shown in the right. (B) Upexpression of miR-211 can significantly promoted the SMMC7721 cells invasion whereas miR-211 inhibitor inhibited SMMC7721 cell invasion. The relative invasive cells of each group have been shown in the right. ***p < 0.001.

Mentions: Overexpression of miR-211 can promote the invasion of HepG2 cells and SMMC7721 compared with the control whereas miR-211 inhibitor inhibited cell invasion (Figure 3A and 3B). The relative invasive cells of each group were shown in the right.


miR-211 suppresses hepatocellular carcinoma by downregulating SATB2.

Jiang G, Cui Y, Yu X, Wu Z, Ding G, Cao L - Oncotarget (2015)

Upregulation of miR-211 inhibits cell invasion(A) Upexpression of miR-211 can significantly promoted the HepG2 cells invasion whereas miR-211 inhibitor inhibitedHepG2cell invasion. The relative invasive cells of each group have been shown in the right. (B) Upexpression of miR-211 can significantly promoted the SMMC7721 cells invasion whereas miR-211 inhibitor inhibited SMMC7721 cell invasion. The relative invasive cells of each group have been shown in the right. ***p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496230&req=5

Figure 3: Upregulation of miR-211 inhibits cell invasion(A) Upexpression of miR-211 can significantly promoted the HepG2 cells invasion whereas miR-211 inhibitor inhibitedHepG2cell invasion. The relative invasive cells of each group have been shown in the right. (B) Upexpression of miR-211 can significantly promoted the SMMC7721 cells invasion whereas miR-211 inhibitor inhibited SMMC7721 cell invasion. The relative invasive cells of each group have been shown in the right. ***p < 0.001.
Mentions: Overexpression of miR-211 can promote the invasion of HepG2 cells and SMMC7721 compared with the control whereas miR-211 inhibitor inhibited cell invasion (Figure 3A and 3B). The relative invasive cells of each group were shown in the right.

Bottom Line: Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues.We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells.This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatopancreatobiliary Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

ABSTRACT
Dysregulation of microRNAs (miRs) is involved in carcinogenesis. Deregulation of miR-211 has recently been observed in many tumors, but its function in hepatocellular carcinoma (HCC) is still unknown. Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues. We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells. Luciferase reporter assays and western blot indicated that special AT-rich sequence-binding protein-2 (SATB2), is a direct target of miR-211. The expression of SATB2 was upregulated in HCC cancer tissues and cell lines and miR-211 levels inversely correlated with SATB2 levels in HCC. Importantly, SATB2 rescued the miR-211-mediated inhibition of cell invasion and proliferation. Finally, reintroduction of miR-211 repressed tumor formation of HCC in xenograft mice. This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

No MeSH data available.


Related in: MedlinePlus