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miR-211 suppresses hepatocellular carcinoma by downregulating SATB2.

Jiang G, Cui Y, Yu X, Wu Z, Ding G, Cao L - Oncotarget (2015)

Bottom Line: Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues.We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells.This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatopancreatobiliary Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

ABSTRACT
Dysregulation of microRNAs (miRs) is involved in carcinogenesis. Deregulation of miR-211 has recently been observed in many tumors, but its function in hepatocellular carcinoma (HCC) is still unknown. Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues. We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells. Luciferase reporter assays and western blot indicated that special AT-rich sequence-binding protein-2 (SATB2), is a direct target of miR-211. The expression of SATB2 was upregulated in HCC cancer tissues and cell lines and miR-211 levels inversely correlated with SATB2 levels in HCC. Importantly, SATB2 rescued the miR-211-mediated inhibition of cell invasion and proliferation. Finally, reintroduction of miR-211 repressed tumor formation of HCC in xenograft mice. This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

No MeSH data available.


Related in: MedlinePlus

miR-211 is downregulated in HCC cell lines and tissues(A) qRT-PCR analysis of miR-211 expression in 40 pairs HCC tissues and their corresponding no tumor tissues. The expression of miR-211 was normalized to U6 snRNA. (B) The expression of miR-211 in HCC tissues was significant lower than in adjacent tissues. (C) The expression of miR-211 in 10 pairs lymph node metastases, HCC tissues and their corresponding to no tumor tissues. (D) Expression levels of miR-211 in four cell lines (MHCC-97H, QGY-7703, SMMC7721 and HepG2) compared with one liver adenocarcinoma cell line, SK-Hep-1, and two adjacent nonneoplastic tissues were detected using qRT-PCR analysis. *p < 0.05 and ***p < 0.001.
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Figure 1: miR-211 is downregulated in HCC cell lines and tissues(A) qRT-PCR analysis of miR-211 expression in 40 pairs HCC tissues and their corresponding no tumor tissues. The expression of miR-211 was normalized to U6 snRNA. (B) The expression of miR-211 in HCC tissues was significant lower than in adjacent tissues. (C) The expression of miR-211 in 10 pairs lymph node metastases, HCC tissues and their corresponding to no tumor tissues. (D) Expression levels of miR-211 in four cell lines (MHCC-97H, QGY-7703, SMMC7721 and HepG2) compared with one liver adenocarcinoma cell line, SK-Hep-1, and two adjacent nonneoplastic tissues were detected using qRT-PCR analysis. *p < 0.05 and ***p < 0.001.

Mentions: The decrease of miR-211 was found in 33 of 40 HCC tissues compared with the corresponding non-tumor tissues (Figure 1A). As shown in Figure 1B, the expression of miR-211 in HCC tissues was lower than in adjacent tissues (Figure 1B, p < 0.001). Moreover, tissues from lymph node metastases also expressed lower levels of miR-211 compared with primary HCC tissues and the adjacent normal tissue (Figure 1C). As shown in Figure 1D, the expression of miR-211 was significantly down-regulated in four cell lines (MHCC-97H, QGY-7703, SMMC7721 and HepG2) compared with one liver adenocarcinoma cell line, SK-Hep-1, and two adjacent non-neoplastic tissues.


miR-211 suppresses hepatocellular carcinoma by downregulating SATB2.

Jiang G, Cui Y, Yu X, Wu Z, Ding G, Cao L - Oncotarget (2015)

miR-211 is downregulated in HCC cell lines and tissues(A) qRT-PCR analysis of miR-211 expression in 40 pairs HCC tissues and their corresponding no tumor tissues. The expression of miR-211 was normalized to U6 snRNA. (B) The expression of miR-211 in HCC tissues was significant lower than in adjacent tissues. (C) The expression of miR-211 in 10 pairs lymph node metastases, HCC tissues and their corresponding to no tumor tissues. (D) Expression levels of miR-211 in four cell lines (MHCC-97H, QGY-7703, SMMC7721 and HepG2) compared with one liver adenocarcinoma cell line, SK-Hep-1, and two adjacent nonneoplastic tissues were detected using qRT-PCR analysis. *p < 0.05 and ***p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496230&req=5

Figure 1: miR-211 is downregulated in HCC cell lines and tissues(A) qRT-PCR analysis of miR-211 expression in 40 pairs HCC tissues and their corresponding no tumor tissues. The expression of miR-211 was normalized to U6 snRNA. (B) The expression of miR-211 in HCC tissues was significant lower than in adjacent tissues. (C) The expression of miR-211 in 10 pairs lymph node metastases, HCC tissues and their corresponding to no tumor tissues. (D) Expression levels of miR-211 in four cell lines (MHCC-97H, QGY-7703, SMMC7721 and HepG2) compared with one liver adenocarcinoma cell line, SK-Hep-1, and two adjacent nonneoplastic tissues were detected using qRT-PCR analysis. *p < 0.05 and ***p < 0.001.
Mentions: The decrease of miR-211 was found in 33 of 40 HCC tissues compared with the corresponding non-tumor tissues (Figure 1A). As shown in Figure 1B, the expression of miR-211 in HCC tissues was lower than in adjacent tissues (Figure 1B, p < 0.001). Moreover, tissues from lymph node metastases also expressed lower levels of miR-211 compared with primary HCC tissues and the adjacent normal tissue (Figure 1C). As shown in Figure 1D, the expression of miR-211 was significantly down-regulated in four cell lines (MHCC-97H, QGY-7703, SMMC7721 and HepG2) compared with one liver adenocarcinoma cell line, SK-Hep-1, and two adjacent non-neoplastic tissues.

Bottom Line: Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues.We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells.This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatopancreatobiliary Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

ABSTRACT
Dysregulation of microRNAs (miRs) is involved in carcinogenesis. Deregulation of miR-211 has recently been observed in many tumors, but its function in hepatocellular carcinoma (HCC) is still unknown. Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues. We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells. Luciferase reporter assays and western blot indicated that special AT-rich sequence-binding protein-2 (SATB2), is a direct target of miR-211. The expression of SATB2 was upregulated in HCC cancer tissues and cell lines and miR-211 levels inversely correlated with SATB2 levels in HCC. Importantly, SATB2 rescued the miR-211-mediated inhibition of cell invasion and proliferation. Finally, reintroduction of miR-211 repressed tumor formation of HCC in xenograft mice. This study provides insights into molecular mechanisms that miR-211 contributed to HCC.

No MeSH data available.


Related in: MedlinePlus